RNDr. Jakub Hofman, Ph.D.

Education and training

2014 - 2015 a six-month research stay in Institut für Toxikologie und Pharmakologie für Naturwissenschaftler, Kiel, Germany (group of Prof. Dr. Edmund Maser)
2008 - 2012

doctoral study (Ph.D. in pharmacology and toxicology; RNDr. in biochemistry)
Charles University in Prague, Faculty of Pharmacy in Hradec Kralove
doctoral thesis: Interactions of cyclin-dependent kinase inhibitors with ABC drug transporters in vitro and in situ, experimental part performed at Department of Pharmacology and Toxicology
supervisor: Prof. PharmDr. Frantisek Staud, Ph.D.

2005-2007

master study (M.Sc. in biochemistry)
Palacky University in Olomouc, Faculty of Science
master thesis: The role of nitric oxide in the defence of plants under biotic and abiotic stress, experimental part performed at Department of Biochemistry, Department of Cell Biology and Genetics and Department of Botany – Phytopathology and microbiology research group
supervisor: Doc. RNDr. Lenka Luhova, Ph.D.

2002 - 2005

bachelor study (B.Sc. in biochemistry)
Palacky University in Olomouc, Faculty of Science
bachelor thesis: Mechanisms of plant defensive reaction under salinity and osmotic stress, experimental part performed at Department of Biochemistry
supervisor: Doc. RNDr. Lenka Luhova, Ph.D.

Positions

2007 - 2008

EMPLA s r.o.
position: environmental chemist
work in analytical laboratories with the focus on GC/MS instrumentation

2008 - 2012

GENERI BIOTECH s r.o.
position: researcher in the field of molecular biology and genetics
research work on various company projects, collaboration with Dr. Petr Cigler and Dr. Miroslav Ledvina from the Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic

2012 - 2015

Charles University in Prague, Faculty of Pharmacy in Hradec Kralove, Department of Biochemical Sciences
position: post-doc research and teaching fellow

2015 - present Charles University in Prague, Faculty of Pharmacy in Hradec Kralove, Department of Pharmacology and Toxicology
position: academic research and teaching fellow

Teaching activities

2017 - present lecturer of Pharmacokinetics
2016 - present lecturer of seminars of Pharmacology
2013 - 2015 lecturer of Practical courses in general biochemistry
2013 - present supervision of 8 M.Sc. students during their master thesis
2009 - present co-supervision of several M.Sc. and Ph.D. students

Research interests

  • pharmacokinetic interactions of novel drug entities with ABC drug efflux transporters and drug metabolizing enzymes
  • pharmacokinetic multidrug resistance
  • anticancer pharmacotherapy
  • identification of new inhibitors of cytosolic carbonyl reducing enzymes
  • transplacental pharmacokinetics of drugs – role of ABC transporters
  • gene delivery using nano-formulated cationic polymers and liposomes

Grants received

Principal investigator 

  • GAUK 114909/C – Study of interactions of CDK inhibitors with ABCG2 (BCRP) drug transporter, Grant Agency of Charles University

Co-investigator

  • KAN200100801 – Bioactive biocompatible surfaces and novel nanostructured composites for applications for medicine and drug delivery, Grant Agency of the Academy of Sciences of the Czech Republic
  • GAUK 137010/C – Localization and function of organic cation transporter 3 (OCT3) in the rat placenta, Grant Agency of Charles University
  • GAUK 700912/C – Study of interactions of cyclin-dependent kinase inhibitors with ABCB1 drug efflux transporter, Grant Agency of Charles University
  • GAČR 16-26849S - Interactions of protein kinase inhibitors with drug transporters and biotransformation enzymes; role in overcoming resistance in anticancer therapy

Memberships

  • International Society for the Study of Xenobiotics (ISSX)
  • Czech Society for Experimental and Clinical Pharmacology and Toxicology

Awards

  • 2010 - finalist for the postdoctoral poster awards competition, 9th International ISSX Meeting, Istanbul, Turkey
  • 2014 - trainee award, 17th International Workshop on the Enzymology and Molecular Biology of Carbonyl Metabolism, Skytop, PA, USA

Publications

  1. Hahnova Cygalova L., Hofman J., Ceckova M., Staud F.: Transplacental pharmacokinetics of glyburide, rhodamine 123, and BODIPY FL Prazosin: Effect of drug efflux transporters and lipid solubility. J Pharmacol Exp Ther 2009; 331 (3): 1118 – 25 (IF for 2009 = 4.309)
  2. Piterkova J., Hofman J., Mieslerova B., Sedlarova M., Luhova L., Lebeda A., Petřivalský M.: Dual role of nitric oxide in Solanum spp.-Oidium neolycopersici interactions. Environ Exp Bot 2011; 74: 37 – 44 (IF for 2011 = 2.985)
  3. Hofman J., Ahmadimoghaddam D., Hahnova L., Pavek P., Ceckova M., Staud F.: Olomoucine II and purvalanol A inhibit ABCG2 transporter in vitro and in situ and synergistically potentiate cytostatic effect of mitoxantrone. Pharmacol Res 2012; 65 (3): 312 – 9 (IF for 2012 = 4.346)
  4. Ahmadimoghaddam D., Hofman J., Zemankova L., Nachtigal P., Dolezelova E., Cerveny L., Ceckova M., Micuda S., Staud F.: Synchronized activity of organic cation transporter 3 (Oct3/SLC22A3) and multidrug and toxin extrusion 1 (Mate 1/SLC47A1) transporter in transplacental passage of MPP+ in rat. Toxicol Sci 2012; 128 (2): 471 – 81 (IF for 2012 = 4.328)
  5. Piterkova J., Luhova L., Hofman J., Tureckova V., Novak O., Petrivalsky M., Fellner M.: Nitric oxide is involved in light-specific responses of tomato during germination under normal and osmotic stress conditions. Ann Bot 2012; 110 (4): 767 - 76 (IF for 2012 = 3.449)
  6. Hofman J., Buncek M., Haluza R., Streinz L., Ledvina M., Cigler P.: In vitro transfection mediated by dendrigraft poly(L-lysines): effect of structure and molecule size. Macromol Biosci 2013; 13 (2): 167 – 76 (IF for 2013 = 3.650)
  7. Hofman J., Kučera R., Cihalova D., Klimes J., Ceckova M., Staud F.: Olomoucine II, but Not Purvalanol A, Is Transported by Breast Cancer Resistance Protein (ABCG2) and P-glycoprotein (ABCB1). PLoS One 2013; 8 (10): e75520 (IF for 2013 = 3.534)
  8. Cihalova D., Hofman J., Ceckova M., Staud F.: Purvalanol A, olomoucine II and roscovitine inhibit ABCB1 transporter and synergistically potentiate cytotoxic effects of daunorubicin in vitro. PLoS One 2013; 8 (12): e83467 (IF for 2013 = 3.534)
  9. Hofman J., Malcekova B., Skarka A., Novotna E., Wsol V.: Anthracycline resistance mediated by reductive metabolism in cancer cells: the role of aldo-keto reductase 1C3. Toxicol Appl Pharmacol 2014; 278 (3): 238 - 48 (IF for 2014 = 3.705)
  10. Skarydova L., Hofman J., Chlebek J., Havrankova J., Kosanova K., Skarka A., Hostalkova A., Plucha T., Cahlikova L., Wsol V.: Isoquinoline alkaloids as a novel type of AKR1C3 inhibitors. J Steroid Biochem Mol Biol 2014; 143: 250 – 8 (IF for 2014 = 3.628)
  11. Hofman J., Skarka A., Havrankova J., Wsol V.: Pharmacokinetic interactions of breast cancer chemotherapeutics with human doxorubicin reductases. Biochem Pharmacol 2015; 96 (3): 168 - 78 (IF for 2015 = 5.091)

  12. Hofman J., Kučera R., Neumanova Z., Klimes J., Ceckova M., Staud F.: Placental passage of olomoucine II, but not purvalanol A, is affected by p-glycoprotein (ABCB1), breast cancer resistance protein (ABCG2) and multidrug resistance-associated proteins (ABCCs). Xenobiotica 2016; 46(5): 416-23 (IF for 2015 = 1.723).

  13. Zemanova L., Hofman J., Novotna E., Musilek K., Lundova T., Havrankova J., Hostalkova A., Chlebek J., Cahlikova L., Wsol V.: Flavones Inhibit the Activity of AKR1B10, a Promising Therapeutic Target for Cancer Treatment. J Nat Prod 2015; 78 (11): 2666 - 74 (IF for 2015 = 3.662)

  14. Hintzpeter J., Seliger J.M., Hofman J., Martin H.J., Wsol V., Maser E.: Inhibition of human anthracycline reductases by emodin - a possible remedy for anthracycline resistance. Toxicol Appl Pharmacol 2016; 293: 21-9 (IF for 2015 = 3.847)

 

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