Mgr. Miloslav Macháček, Ph.D.

Date and Place of Birth: 7 December 1987, Prague, Czech Republic

Present Position:

Contact Information:

  • Work Address: Akademika Heyrovského 1203/8, 500 05 Hradec Králové, Czech Republic
  • E-mail: machamil@faf.cuni.cz
  • Tel.: +420 495 067 588

Previous Education and Professional Experience

Research Interests

  • photodynamic therapy of cancer
  • mode of cellular death
  • oxidative stress on cellular and molecular level
  • evaluation of toxicological and pharmacological properties of novel phthalocyanine and azaphthalocyanine (tetrapyrazinoporphyrazine and tetrapyridoporphyrazine) photosensitizers
  • fluorescence and confocal microscopy
     
  • list of publications , see below

 

Grant projects

  • principal investigator, Grant Agency of Charles University, GAUK 1916214, “Study of the novel phthalocyanine and azaphthalocyanine photosensitizers for photodynamic therapy of tumors on cellular and molecular level.” (2014-2016)
  • co-investigator in other research grants and projects (1× GA CR, 4× GA UK, 1× UNCE)

Professional membership

Educational activities

  • supervisor of 8 B.Sc. and 9 M.Sc. (Pharmacy and Medical Bioanalytics) diploma theses
  • Pathological Biochemistry - lectures, seminaries
  • Molecular Biology – practice
  • General Biochemistry - practice
  • Biophysics - practice (2012-2013)

Hobbies / Personal Interests

  • natural sciences
  • wild-life and macrophotography, astrophotography
  • nature protection, conservation & preservation
  • growing carnivorous plants
PTX6128_PS.jpg PTX_0080_PS-(Monte-Pelmo).JPG
Adult click beatle Actenicerus siaelandicus. Czech Republic Adult male jumping spider Evarcha arctuata. Czech Republic Last sunrays of the day struggle through clouds and touching Monte Pelmo, Dolomites, Italy.
PTX_8871_PS_-_PFS.JPG PTX_4010_Drosera_graomogolensis_v0.jpg

Critically endangered European ground squirrel alias European souslik (Spermophilus citellus). Czech Republic

Mediterranean male dragonfly Violet Dropwing (Trithermis annulata) displays interesting mauve colouration. Turkey.

South-american sundew Drosera graomogolensis.
Ori_102B302B60_PS2BSharp_Lumi_PS3_PFS.jpg
Bright emmision nebulae in the constellation Cygnus (Swan) - North America (NGC7000) and Pelican (IC5067) nebulae. Powerful positive cloud-to-ground lightning strike behind Bezděz castle, Czech Republic. Toronto during a nightfall from Toronto Islands, Ontario, Canada.

Publications

  • author and co-author of 17 publications in international peer-reviewed journals
  • cumulative IF > 70; more than 101/84 citations (WoS/Scopus; excluding self-citations); H-index: 5/6 (WoS/Scopus)
  • green marked publications - photodynamic activity of phthalocyanines and their aza-analogues
     

2017

  • Kopečná M, Macháček M, Prchalová E, Štěpánek P, Drašar P, Kotora M, Vávrová K.
    Galactosyl Pentadecene Reversibly Enhances Transdermal and Topical Drug Delivery.
    Pharmaceutical Research. 2017; accepted
    [Impact factor 2016: 3.002]

  • Ghazal B, Machacek M, Shalaby M, Novakova V, Zimcik P, Makhseed.
    Phthalocyanines and tetrapyrazinoporphyrazines with two cationic donuts: high photodynamic activity as a result of rigid spatial arrangement of peripheral substituents.
    Journal of Medicinal Chemistry. 2017; DOI: 10.1021/acs.jmedchem.7b00272

    [Impact factor 2016: 6.259]

  • Němeček J, Sychra P, Macháček M, Benková M, Karabanovich G, Konečná K, Kavková V, Stolaříkov J, Hrabálek A, Vávrová K, Soukup O, Roh J, Klimešová V.
    Structure-activity relationship studies on 3,5-dinitrophenyl tetrazoles as antitubercular agents.

    European Journal of Medicinal Chemistry. 2017; 130(21): 419–432
    [Impact factor 2016: 4.519]

  • Kopečná M, Macháček M, Prchalová E, Štěpánek P, Drašar P, Kotora M, Vávrová K.
    Dodecyl amino glucoside enhances transdermal and topical drug delivery via reversible interaction with skin barrier lipids.
    Pharmaceutical Research. 2017; DOI: 10.1007/s11095-016-2093-z
    [Impact factor 2016: 3.002]

2016

  • Machacek M, Demuth J, Cermak P, Vavreckova M, Hruba L, Jedlickova A, Kubat P, Simunek T, Novakova V, Zimcik P.
    Tetra(3,4-pyrido)porphyrazines caught in the cationic cage: toward nanomolar active photosensitizers.

    Journal of Medicinal Chemistry. 2016; 59(20): 9443–9456
    [Impact factor 2016: 6.259]

  • Haškova P, Jansova H, Bureš J, Macháček M, Jirkovská A, Franz KJ, Kovaříkova P, Šimůnek T.
    Cardioprotective effects of iron chelator HAPI and ROS-activated boronate prochelator BHAPI against catecholamine-induced oxidative cellular injury.
    Toxicology. 2016; 371: 17-28. DOI: 10.1016/j.tox.2016.10.004
    [Impact Factor 2016 = 3.582]

  • Jansová H, Bureš J, Macháček M, Hašková P, Jirkovská A, Roh J, Wang Q, Franz KJ, Kovaříková P, Šimůnek T.
    Characterization of cytoprotective and toxic properties of iron chelator SIH, prochelator BSIH and their degradation products.

    Toxicology. 2015; 250: 15-24
    [Impact Factor 2016 = 3.582]

  • Machacek M, Kollár J, Miletin M, Kučera R, Kubát P, Simunek T, Novakova V, Zimcik P.
    Anionic hexadeca-carboxylate tetrapyrazinoporphyrazine: synthesis and in vitro photodynamic studies of water-soluble non-aggregating photosensitizer.
    RSC Advances. 2016, DOI: 10.1039/C5RA25881B

    [Impact factor 2016: 3.289]

2015

  • Potůčková E, Roh J, Macháček M, Stariat J, Šesták V, Jansová H, Hašková P, Jirkovská A, Vávrová A, Kovaříková P, Richardson DR, Šimůnek T.
    In vitro characterization of pharmacological properties of the anticancer iron chelator Bp4eT and its phase I metabolites.
    PLoS One. 2015; 10(10): e0139929. DOI:10.1371/journal.pone.0139929
    [Impact factor 2015: 3.234]

  • Jirkovská-Vávrová A, Roh J, Lenčová-Popelová O, Jirkovský E, Hrušková K, Potůčková-Macková E, Jansová H, Hašková P, Martinková P, Eisner T, Kratochvíl M, Šůs J, Macháček M, Vostatková-Tichotová L, Geršl V, Muller MT, Richardson DR, Vávrová K, Štěrba M, Šimůnek T.
    Synthesis and analysis of novel analogues of dexrazoxane and its open-ring hydrolysis product for protection against anthracycline cardiotoxicity in vitro and in vivo.
    Toxicology Research. 2015; 4: 1098-1114

    [Impact factor 2016: 2.161]

  • Vachova L, Machacek M, Kucera R, Demuth J, Cermak P, Kopecky K, Miletin M,  Jedlickova A, Simunek T, Novakova V and Zimcik P.
    Heteroatom-substituted tetra(3,4-pyrido)-porphyrazines: a stride toward near-infrared-absorbing macrocycles.
    Organic & Biomolecular Chemistry. 2015; DOI: 10.1039/c5ob00651a
    [Impact factor 2016: 3.559]

  • Machacek M, Cidlina A, Novakova V, Svec J, Rudolf E,Miletin M, Kučera R, Simunek T, Zimcik P.
    Far-red absorbing cationic phthalocyanine photosensitizers: Synthesis and evaluation of the photodynamic anti-cancer activity and the mode of cell death induction.
    Journal of Medicinal Chemistry. 2015; 58(4): 1736–1749

    [Impact factor 2016: 6.259]

2014

  • Potůčková E, Hrušková K, Bureš J, Kovaříková P, Špirková IA, Pravdíková K, Kolbabová L, Hergeselová T, Hašková P, Jansová H, Macháček M, Jirkovská A, Richardson V, Lane DJR, Kalinowski DS, Richardson DR, Vávrová K, Šimůnek T. 2014.
    Structure-activity relationships of novel salicylaldehyde isonicotinoyl hydrazone (SIH) analogs: iron chelation, anti-oxidant and cytotoxic properties.
    PLoS One. 2014; 9(11): e112059.

    [Impact factor 2015: 3.234]

  • Jansová H, Macháček M, Wang Q, Hašková P, Vávrová A, Potůčková E, Kielar F, Franz KJ, Šimůnek T. 2014.
    Comparison of various iron chelators and prochelators as protecting agents against cardiomyocyte oxidative injury.
    Free Radical Biology & Medicine. 2014. doi: 10.1016/j.freeradbiomed.2014.06.019.

    [Impact factor 2015: 5.606]

  • Potuckova E, Jansova H, Machacek M, Vavrova A, Haskova P, Tichotova L, Richardson V, Kalinowski DS, Richardson DR, Simunek T..
    Quantitative analysis of the anti-proliferative activity of combinations of selected iron-chelating agents and clinically used anti-neoplastic drugs. 
    PLoS One. 2014. 9(2). e88754. doi:10.1371/journal.pone.0088754.

    [Impact factor 2015: 3.234]

2013

  • Makhseed S, Machacek M, Alfadly W, Tuhl A, Vinodh V, Simunek T, Novakova V, Kubat P, Rudolf E a Zimcik P.
    Water-soluble non-aggregating zinc phthalocyanine and in vitro studies for photodynamic therapy.
    Chemical Communications. 2013. 49(95). Pages 11149-51

    [Impact factor 2016: 6,567]

  • Vávrová A, Jansová H, Macková E, Macháček M, Hašková P, Tichotová L, Štěrba M, Šimůnek T.
    Catalytic inhibitors of topoisomerase II differentially modulate the toxicity of anthracyclines towards cardiac and cancer cells.
    PLoS ONE. 2013. 8(10). Pages 1-13

    [Impact factor 2015: 3.234]

© 2017 Charles University, Faculty of Pharmacy in Hradec Králové, Akademika Heyrovského 1203, 500 05 Hradec Králové, Czech Republic
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