Prof. Ing. Barbora Szotáková, Ph.D.

Doc. Ing. Barbora Szotáková, Ph.D.

Present position:

Contact information:

  • Work Address: Charles University, Faculty of Pharmacy, Heyrovského 1203, 500 05 Hradec Králové, Czech Republic
  • E-mail: barbora.szotakova@faf.cuni.cz
  • Tel.: +420 495 067 324

 

Previous Education and Training

  • Since 2016: Professor at the Department of Biochemical Sciences, Faculty of Pharmacy in Hradec Králové, Charles University in Prague 
  • 2007-2016: Associate Professor at the Department of Biochemical Sciences, Faculty of Pharmacy in Hradec Králové, Charles University in Prague 
  • 2006: Habilitation in Biochemistry
  • 2001: Ph.D. degree in Pathobiochemistry and Xenobiochemistry
  • 2000 - 2003: Short internships in Institute of Pharmacology and Toxicology, Philipps-University of Marburg,  Germany
  • 1988 - 2006: Assistant professor at the Department of Biochemical Sciences, Faculty of Pharmacy in Hradec Králové, Charles University in Prague
  • 1982 - 1988: Institute of Chemical Technology, Prague, Faculty of Food and Biochemical Technology

Research Interest

  • Study of drug metabolizing enzymes (cytochromes P450 - CYP1A, 2B, 2C, 2D6, 2E1, 3A; flavine monooxygenases; carbonyl reducing enzymes; glutathione S-transferases; UDP-glucuronosyl transferases…)
  • Study of enzymes of oxidative stress (peroxidase, glutathione peroxidase, catalase, superoxide dismutase, thioredoxin reductase…)
  • In
    • human
    • farm animals and free living animals
    • laboratory animals
    • tumor cell lines
    • parasiting helminths
    • plants
  • Activities and properties of these enzymes
  • Their modulation (induction, inhibition) by drugs, dietary supplements and environmental contaminants
  • Identification of metabolites of xenobiotics
  • Pharmacokinetics of drugs in target animal species

Teaching

Professional Memberships

  • International Society for the Study of Xenobiotics (ISSX)
  • Czech Society for Biochemistry and Molecular Biology (CSBMB)
  • Czech Science Foundation (GAČR), P503 – Food technology, ecotoxicology and environmental chemistry
  • Head of the Ethical Committee of the Charles University in Prague, Faculty of Pharmacy in Hradec Králové

Grants Received

  • 2015-2017: GAČR P503/15-05325S, Anthelmintics in plants – uptake, biotransformation and transcriptional response
  • 2007-2009: GAČR 524/07/0611, Dicrocoeliosis in farm animals - defence mechanisms of lancet fluke against anthelmintics
  • 2005-2006: GAUK 108/2005/C, Interakce veterinárních anthelmintik a reduktas karbonylové skupiny
  • 2001: GAUK 108/2005/C, Interakce veterinárních anthelmintik a reduktas karbonylové skupiny
  • 2000-2002: GAUK 96/2000/C, Studium biotransformace potenciálních cytostatik ve střevě potkana. Význam intestinální mikroflóry
  • 1997-1999: GAUK 113/97/C, Studie vzájemných vztahů mikrosomálních monooxygenas a potenciálních cytostatik

   Member of further grants GAČR, GAUK, FRVŠ, MŠMT

Publications

 Number of publications: 93
 Number of citations:   781
 H-index: 15

 

Selected publications in past five years:

  1. Nobilis M., Vybíralová Z., Szotáková B., Sládková K., Kuneš M., Svoboda Z. High-performance liquid chromatographic determination of tiapride and its phase I metabolite in blood plasma using UV photodiode-array and fluorescence detection in tandem. Application to pharmacokinetic studies. J. Chromatogr. B 879 (2011) 3845-3852 (doi:10.1016/j.jchromb.2011.10.032) [Impact factor 2011: 2,888]
     
  2. Vokřál, I., Jirásko, R., Jedličková, V., Bártíková, H., Skálová, L., Lamka, J., Holčapek, M., Szotáková, B. The inability of tapewormHymenolepis diminuta and fluke Dicrocoelium dendriticum to metabolize praziquantel. Vet. Parasitol. 185 (2012) 168-174. (doi:10.1016/j.vetpar.2011.09.026) [Impact factor 2011: 2,579]
     
  3. Bártíková, H., Vokřál, I., Skálová, L., Kubíček, V., Firbasová, J., Briestenský, D., Lamka, J., Szotáková, B. Activity of drug metabolizing enzymes and biotransformation of selected anthelmintics in model tapeworm Hymenolepis diminuta. Parasitology 139 (2012) 809-818. (doi:10.1017/S0031182011002265) [Impact factor 2011: 2,961]
     
  4. Bártíková, H., Vokřál, I., Kubíček, V., Szotáková, B., Prchal, L., Lamka, J., Várady, M., Skálová, L. Import and efflux of flubendazole in Haemonchus contortus strains susceptible and resistant to anthelmintics. Vet. Parasitol. 187 (2012) 473-479 (doi:10.1016/j.vetpar.2012.02.008). [Impact factor 2012: 2.579]
     
  5. Vokřál, I., Bártíková, H., Prchal, L., Stuchlíková, L., Skálová, L., Szotáková, B., Lamka, J., Várady, M., Kubíček, V. The metabolism of flubendazole and the activities of selected biotransformation enzymes in Haemonchus contortus strains susceptible and resistant to anthelmintics. Parasitology 139 (2012) 1309-1316 (doi:10.1017/S0031182012000595). [Impact factor 2011: 2.961]
     
  6. Vokřál, I., Jedličková, V., Jirásko, R., Stuchlíková, L., Bártíková, H., Skálová, L., Lamka, J., Holčapek, M., Szotáková, B. The metabolic fate of ivermectin in host (Ovis aries) and parasite (Haemonchus contortus). Parasitology 140 (2013) 361–367 (doi:10.1017/S0031182012001680). [Impact factor 2011: 2.961]
     
  7. Stuchlíková L., Jirásko R., Vokřál I., Lamka J., Spulák M., Holčapek M., Szotáková B., Bártíková H., Pour M., Skálová L. Investigation of the metabolism of monepantel in ovine hepatocytes by UHPLC/MS/MS. Anal Bioanal Chem. 405 (2013) 1705–1712 (doi:10.1007/s00216-012-6584-4). [Impact factor 2012: 3.778]
     
  8. Szotáková, B., Bártíková, H., Hlaváčová, J., Boušová, I., Skálová, L. Inhibitory effect of anthocyanidins on hepatic glutathione S-transferase, UDP-glucuronosyl transferase and carbonyl reductase activities in rat and human. Xenobiotica 43 (2013) 679-685 (doi:10.3109/00498254.2012.756557). [Impact factor 2012: 1.791]
     
  9. Vokřál, I., Jirásko, R., Stuchlíková, L., Bártíková, H., Szotáková, B., Lamka, J., Várady, M., Skálová, L. Biotransformation of albendazole and activities of selected detoxification enzymes in Haemonchus contortus strains susceptible and resistant to anthelmintics. Vet. Parasitol. 196 (2013) 373–381 (doi: 10.1016/j.vetpar.2013.03.018). [Impact factor 2012: 2.381]
     
  10. Podlipná, R., Skálová, L., Seidlová, H., Szotáková, B., Kubíček, V., Jirásko, R., Vaněk, T., Vokřál, I. Biotransformation of benzimidazole anthelmintics in reed (Phragmites australis) as a potential tool for their detoxification in environment. Bioresource Technology 144 (2013) 216-224 (doi: 10.1016/j.biortech.2013.06.105). [Impact factor 2012: 4.750]
     
  11. Vyskočilová, E., Szotáková, B., Skálová, L., Bártíková, H., Hlaváčová, J., Boušová, I. Age-related changes in hepatic activity and expression of detoxification enzymes in male rats. BioMed Research International (2013), art. no. 408573 (doi:10.1155/2013/408573). [Impact factor 2012: 2.880]
     
  12. Matoušková, P., Bártíková, H., Boušová, I., Hanušová, V., Szotáková, B., Skálová, L. Reference genes for Real-Time PCR quantification of messenger RNAs and microRNAs in mouse model of obesity. PLoS ONE 9(1) (2014): e86033. (doi:10.1371/journal.pone.0086033. [Impact factor 2013: 3.530]
     
  13. Bártíková, H., Boušová, I., Jedličková, P., Lněničková, K., Skálová, L., Szotáková, B. Effect of standardized cranberry extract on the activity and expression of selected biotransformation enzymes in rat liver and intestine. Molecules 19 (2014) 14948-14960 (doi:10.3390/molecules190914948). [Impact factor 2013: 2.095]
     
  14. Stuchlikova, L., Jirasko, R., Vokral, I., Valat, M., Lamka, J., Szotakova, B., Holcapek, M., Skalova, L. Metabolic pathways of anthelmintic drug monepantel in sheep and in its parasite (Haemonchus contortus). Drug testing and analysis 6 (10) (2014) 1055 -1062 (doi:10.1002/dta.1630). [Impact factor 2013: 2.816]
     
  15. Matoušková, P., Bártíková H., Boušová, I., Szotáková, B., Martin, J., Skorkovská, J., Hanušová, V., Tománková, V., Anzenbacherova, E., Lišková, B., Anzenbacher, P., Skálová L. Effect of defined green tea extract in various dosage schemes on drug-metabolizing enzymes in mice in vivo. Journal of Functional Foods 10 (2014) 327-335 (doi:10.1016/j.jff.2014.06.026). [Impact factor 2013: 4.480]
     
  16. Prchal L., Bártíková H., Bečanová A., Jirásko R., Vokřál I., Stuchlíková L., Skálová L., Kubíček V., Lamka J., Trejtnar F., Szotáková B. Biotransformation of anthelmintics and the activity of drug metabolizing enzymes in the tapeworm Moniezia expansa. Parasitology142 (5) (2015) 648-659 (doi:10.1017/S0031182014001711). [Impact factor 2013: 2.350]
     
  17. Ambrož, M., Boušová, I., Skarka, A., Hanušová, V., Králová, V., Matoušková, P., Szotáková, B., Skálová, L. The influence of sesquiterpenes from Myrica rubra on the antiproliferative and pro-oxidative effects of doxorubicin and its accumulation in cancer cells. Molecules 20 (8) (2015) 15343-15358. (doi:10.3390/molecules200815343). [Impact factor 2014: 2.146]

  18. Stuchlíková, L., Matoušková, P., Bártíková, H., Vokřál, I., Lamka, J., Štolcová, T., Pětníková, H., Szotáková, B., Kubíček, V., Skálová, L. Monepantel induces hepatic cytochromes P450 in sheep in vitro and in vivo. Chem.-Biol. Interact. 227 (2015) 63-68. (doi:10.1016/j.cbi.2014.12.025). [Impact factor 2014: 2.577]

  19. Bousova, I., Bartikova, H., Matouskova, P., Lnenickova, K., Zappe, L., Valentova, K., Szotakova, B., Martin, J., Skalova, L. Cranberry extract-enriched diets increase NAD(P)H:quinone oxidoreductase and catalase activities in obese but not in nonobese mice. Nutrition Res. (2015) 901-909. (doi:10.1016/j.nutres.2015.08.002). [Impact factor 2014: 2.472]

  20. Lecova, L., Ruzickova, M., Laing, R., Vogel, H., Szotakova, B., Prchal, L., Lamka, J., Vokral, I., Skalova, L., Matouskova, P. Reliable reference gene selection for quantitative real time PCR in Haemonchus contortus. Mol. Biochem. Parasitol. 201 (2) (2015) 123-127. (doi:10.1016/j.molbiopara.2015.08.001). [Impact factor 2014: 1.787]

  21. Bartikova, H., Skalova, L., Valentova, K., Matouskova, P., Szotakova, B., Martin, J., Kvita, V., Bousova, I. Effect of oral administration of green tea extract in various dosage schemes on oxidative stress status of mice in vivo. Acta Pharm. 65 (1) (2015) 65-73. (doi:10.1515/acph-2015-0007). [Impact factor 2014: 0.912]

  22. Matouskova, P., Bartikova, H., Bousova, I., Levorova, L., Szotakova, B., Skalova, L. Drug-Metabolizing and Antioxidant Enzymes in Monosodium L-Glutamate Obese Mice. Drug Metab. Dispos. 43 (2) (2015) 258-265. (doi:10.1124/dmd.114.061176). [Impact factor 2014: 3.252]

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