Equipment and Methods

Chemistry section

  • Rational Drug Design, Computer Aided Drug Design (CADD), molecular modelling, molecular docking, pharmacophore models
  • Using MOE (Molecular Operating Environment) software package developed by Chemical Computing Group, Montreal, QC, Canada
  • Microwave assisted organic synthesis (Microwave reactor CEM Discover with autosampler Exlorer 24)
  • Preparative flash chromatography (Combiflash Rf, Teledyne Isco)
  • Calculations, prediction and experimental measurement of physico-chemical properties (mainly log P,  pKa)
  • Structure-Activity Relationships (SAR)
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Group of microbiology and immunology

  • Microscopy - confocal laser scanning microscope - Nikon Eclipse Ti, fluorescence microscope Olympus BX40
  • Cultivation of microbes - CO2 incubator, microbiological incubators, autoclaves, biohazard safety boxes, anaerostat
  • Mapping of the growth of microbes - culture growth monitoring instrument - Bioscreen C, spectrophotometer

Methods

  • Microdilution broth method, disc diffusion test, E-test, aerobic/microaerobic/anaerobic cultivation of microbes, microbial population counting, mapping of growth curves.
  • In vitro biofilm formation, biofilm quantification – spectrophotometrically; detection, visualization – fluorescent, confocal microscopy.
  • Evaluation of metabolic activity of microbes – spectrophotometrically, microbial viability evaluation – plate count method, bright field/fluorescent microscopy.
  • Microbial protein isolation, concentration and purification: Stirred cell, Amicon, Centricon (Millipore) tubes, sucrose gradient; preparation of protein samples for MS analysis – sample desalting, protein separation and visualization: 1 D SDS-PAGE, Coomassie blue staining, sweet silver staining – 1 D gel analysis; in-gel trypsin digest, extraction/concentration of peptides.

Equipment

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Cultivation of microbes - CO2 incubator, microbiological incubators, autoclaves, biohazard safety boxes, anaerostat.

 

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Mapping of the growth of microbes - culture growth monitoring instrument - Bioscreen C, spectrophotometer.

 

And other: vortexes, OD meters, microcentrifuge, cooled centrifuge, Stirred cell for sample/protein concentration, microbiology shakers, sonification bath, ...

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