Assoc. Prof. Ing. Kateřina Macáková, Ph.D.

Research focus

  • biological activity of plant extracts and compounds isolated from them - antioxidant activity, chelation of transition metals, inhibition of various enzymes, antiaggregatory activity


  • 2019, Charles University, Faculty of Pharmacy in Hradec Králové, field: Pharmacognosy
  • 2011 Ph.D., Charles University in Prague, Faculty of Pharmacy in Hradec Králové, field: Pharmacognosy and toxicology of natural substances
  • 2004 MSc., Czech University of Life Sciences, Institut of Tropics and Subtropics, field: Plant production


  • 2007-2019: Charles University, Faculty of Pharmacy in Hradec Králové, Department of Pharmaceutical Botany
  • from 2019: Charles University, Faculty of Pharmacy in Hradec Králové, Department of Pharmacognosy, current position: head of department

Teaching activities

  • Pharmaceutical botany (lectures and practicals)
  • Production of medicinal plants (lectures and practicals)
  • Pharmacognosy (lectures)


  • 2012 Prize of Danone Institute  – The prize of a young scientist (2.-3. prize)

Research fellowships

  • 2019 research fellowship at the Department of Plant Production (Laboratory of Tropical and Subtropical Agriculture and Ethnobotany, Ghent University (1 month)
  • 2015 research fellowship at the Department of Pharmacology and the Department of Pharmacognosy, University Pecs (2 months)



POUROVA J, APPLOVA L, MACAKOVA K, VOPRSALOVA M, MIGKOS T, BENTANACHS R, BIEDERMANN D, PETRASKOVA L, TVRDY V, HRUBSA M, KARLICKOVA J, KREN V, VALENTOVA K, MLADENKA P. The effect of silymarin flavonolignans and their sulfated conjugates on platelet aggregation and blood vessels ex vivo. Nutrients 2019;11(10): 2286. IF2018=4.2

APPLOVA L, KARLICKOVA J, WARNCKE P, MACAKOVA K, HRUBSA M, MACHACEK M, TVRDY V, FISCHER D, MLADENKA P. 4-Methylcatechol, a flavonoid metabolite with potent antiplatelet effects. Mol Nutr Food Res. 2019; 63 (20): 1900261.  IF2017=5.2

MIGKOS T, APPLOVA L, HORKÝ P, TVRDY V, KARLICKOVA J, MACAKOVA K, HRUBSA M, CATAPANO MC, TOMANEK M, POUR M., MLADENKA P. The influence of microbial isoflavonoid specific metabolites on platelets and transition metals iron and copper. Phytomedicine 2019;62:152974. IF2017=3.6

MACAKOVA K, AFONSO R, SASO L, MLADENKA P. The influence of alkaloids on oxidative stress and related signaling pathways. Free Rad Biol Med 2019;134: 429-444. IF2017=6.0

MACAKOVA, K., M.C. CATAPANO, V. TVRDY, K. KLIMKOVA, J. KARLICKOVA, P. MLADENKA. Hematoxylin assay of cupric chelation can give false positive results. Journal of Trace Elements in Medicine and Biology, 2019, vol. 52, pp.29-36. IF2017=3.755


APPLOVA, L., E. VELJOVIC, S. MURATOVIC, J. KARLICKOVA, K. MACAKOVA, D. ZAVRSNIK, L. SASO, K. DURIC, R. HRDINA, P. MLADENKA. 9-(4'-dimethylaminophenyl)-2,6,7-trihydroxy-xanthene-3-one is a potentially novel antiplatelet drug antagonizing the effect of thromboxane A2. Medicinal Chemistry, 2018, vol. 14, no. 2, pp. 200-209. IF2017=2.631


APPLOVA, L., J. KARLICKOVA, M. RIHA, T. FILIPSKY, K. MACAKOVA, J. SPILKOVA, P. MLADENKA. The isoflavonoid tectorigenin has better antiplatelet potential than acetylsalicylic acid. Phytomedicine, 2017, vol. 35, pp. 11-7. IF2016=3.526


DOSKOCIL, I., J. HAVLIK, R. VERLOTTA, J. TAUCHEN, L. VESELA, K. MACAKOVA, L. OPLETAL, L KOKOSKA, V. RADA. In vitro immunomodulatory activity, cytotoxicity and chemistry of some central European polypores.  Pharmaceutical Biology, 2016, vol. 54, pp. 2369-2376. IF2015=1.546

CHLEBEK, J., Z. NOVAK, D. KASSEMOVA., M. SAFRATOVA, J. KOSTELNIK, L. MALY, M. LOCAREK, L. OPLETAL, A. HOSTALKOVA, M. HRABINOVA, J. KUNES, P. NOVOTNA, M. URBANOVA, L. NOVAKOVA, K. MACAKOVA, D. HULCOVA, P. SOLICH, C.P. MARTIN, D. JUN, L. CAHLIKOVA. Isoquinoline alkaloids from Fumaria officinalis L. and their biological activities related to Alzheimer’s disease. Chemistry & Biodiversity, 2016, vol. 13, pp. 91-99. IF2015=1.444

KARLICKOVA, J., M. RIHA, T. FILIPSKY, K. MACAKOVA, R. HRDINA, P. MLADENKA. Antiplatelet effects of flavonoids mediated by inhibition of arachidonic acid based pathway. Planta MedIca, 2016, vol. 82, no. 01/02, pp. 76-83. IF2015=1.99


CAHLIKOVA, L., L. HULOVA., M. HRABINOVA, J. CHLEBEK, A. HOSTALKOVA, M. ADAMCOVA, M. SAFRATOVA, D. JUN, L. OPLETAL, M. LOCAREK, K. MACAKOVA: Isoquinoline alkaloids as potential prolyl oligopeptidase inhibitors. Fitoterapia,  2015, vol.103, pp. 192-196. IF2014=2.354

FILIPSKÝ, T., M. RIHA, K. MACAKOVA, E. ANZENBACHEROVA, J. KARLICKOVA, P. MLADENKA. Antioxidant effects of coumarins include direct radical scavenging, metal chelation and inhibition of ROS-producing enzymes. Current Topics in Medicinal Chemistry, 2015, vol. 15, pp. 415-431. IF2014=3.402

HAVLIKOVA, L., K. MACAKOVA, L. OPLETAL, P. SOLICH. Rapid determination of alpha-hederin and hederacoside c in extracts of Hedera helix leaves available in the Czech republic and Poland. Natural Product Communications, 2015, vol. 10, pp. 1529-1531. IF2014=0.906

KARLICKOVA, J., K. MACAKOVA, M. RIHA, L.M.T. PINHEIRO, T. FILIPSKY, V. HORNASOVA, R. HRDINA, P. MLADENKA. Isoflavones reduce copper with minimal impact on iron in vitro. Oxidative Medicine and Cellular Longevity,  2015, article ID 437381. IF2014=3.516


CAHLIKOVA, L., K. MACAKOVA, N. BENESOVA, J. CHLEBEK, A. HOSTALKOVA, L. OPLETAL. Natural compounds (small molecules) as potential and real drugs of Alzheimer's disease: a critical review. In Studies in natural products chemistry (Bioactive natural products), Elsevier, 2014, vol. 42, s. 153-194, ISBN 978-044463281-4

REHAKOVA Z., V. KOLECKAR, L. JAHODAR, L. OPLETAL, K. MACAKOVA, L. CAHLIKOVA, D. JUN,  K. KUCA. Evaluation of the antioxidant activity of several naturally occurring coumarins and their synthesized analogues by “ferric reducing antioxidant power” assay. Journal of Enzyme Inhibition and Medicinal Chemistry, 2014, vol. 29, pp. 49-54. IF2013=2.383

RIHA M., J. KARLICKOVA, T. FILIPSKY, K. MACAKOVA, L. ROCHA, P. BOVICELLI, I.P. SILVESTRI, L. SASO, L. JAHODAR, R. HRDINA, P. MLADENKA. In vitro evaluation of copper-chelating properties of flavonoidpp. RSC Advances, 2014, vol. 4, pp. 32628-32638. IF2013=3.708

SAFRATOVA M., Z. NOVAK, A. KULHANKOVA, J. KUNES, M. HRABINOVA, D. JUN, K. MACAKOVA, L. OPLETAL, L. CAHLIKOVA. Revised NMR data for 9-O-demethylgalanthine: an alkaloid from Zephyranthes robusta (Amaryllidaceae) and its biological aktivity Natural Product Communications, 2014, vol. 9, pp. 787-788. IF2013=0.924


CAHLIKOVA L., M. HRABINOVA, A. KULHANKOVA, N. BENESOVA, J. CHLEBEK, D. JUN, Z. NOVAK, K. MACAKOVA, J. KUNES, K. KUCA, L. OPLETAL. Alkaloids from Chlidanthus fragrans and thei acetylcholinesterase, butyrylcholinesterase and prolyl oligopeptidase activities. Natural Product Communications,  2013, vol. 8, no. 11, pp. 1541-1544. IF2012=0.956

HALAMOVA, K., L. KOKOSKA, Z. POLESNY, K. MACAKOVA, J. FLESAR, V. RADA. Selective in vitro growth inhibitory activity of Withania somnifera on human pathogenic bacteria and bifidobacteria. Pakistan Journal of Botany, 2013, vol. 45, no. 2, pp. 667-670. IF2012=0.872

KULHANKOVA, A., L. CAHLIKOVA, Z. NOVAK, K. MACAKOVA, J. KUNES, L. OPLETAL. Alkaloids from Zephyranthes robusta Baker and their acetylcholinesterase and butyrylcholinesterase inhibitory activity. Chemistry & Biodiversity, 2013, vol. 10, no. 6, pp. 1120-1127. IF2012=1.808

MACAKOVA, K., V. KOLECKAR, L. CAHLIKOVA, J. CHLEBEK, A. HOSTALKOVA, K. KUCA, D. JUN, L. OPLETAL. Chapter 9 Tannins and their influence on health. In Recent advances in medicinal chemistry, Bentham Science Publishers, 2013, vol. 1, s. 249-293 ,ISBN 978-1-60805-797-9

OPLETAL, L., J .CHLEBEK, K. MACAKOVA, N. BENESOVA, A. HOSTALKOVA, L. CAHLIKOVA. Antioxidanty a degenerativní onemocnění. Praktické lékárenství, 2013, vol. 9, no. 3, pp. 135-138.

RIHA M., J. KARLICKOVA, T. FILIPSKY, K. MACAKOVA, R. HRDINA, P. MLADENKA. Novel method for rapid copper chelation assessment confirmed low affinity of D-penicillamine for copper in comparison with trientine and 8-hydroxyquinolines. Journal of Inorganic Biochemistry, 2013, vol. 123, pp. 80-87. IF2012=3.197


CAHLIKOVA, L., N.  BENESOVA, K. MACAKOVA, R. KUCERA, V. HRSTKA, J. KLIMES, L. JAHODAR, L. OPLETAL. Alkaloids from Some Amaryllidaceae Species and Their Cholinesterase Activity. Natural Product Communications, 2012, vol. 7, no. 5, pp. 571-574. IF2011=1.242

FILIPSKY T., P. MLADENKA, K. MACAKOVA, R. HRDINA, L. SASO, F. MARCHETTI, C. PETTINARI. In vitro chracteristics of 1-phenyl-3-methyl-4-acylpyrazol-5-ones iron chelators. Biochimie, 2012, vol. 94, pp. 125-131. IF2011=3.022

MACAKOVA, K., P. MLADENKA, T. FILIPSKY, M. RIHA, L. JAHODAR, F. TREJTNAR, P. BOVICELLI, I.P. SILVESTRI, R. HRDINA, L. SASO. Iron reduction potentiates hydroxyl radical formation only in flavonols. Food Chemistry, 2012, vol. 135, pp. 2584-2592. IF2011=3.655

MACAKOVA, K., Z. REHAKOVA, P. MLADENKA, J. KARLICKOVA, T. FILIPSKY, M. RIHA, A.K. PRASAD, V.S. PARMAR, L. JAHODAR, P. PAVEK, R. HRDINA, L. SASO. In vitro platelet antiaggregatory properties of 4-methylcoumarins. Biochimie, 2012, vol. 94, pp. 2681-2686. IF2011=3.022

KOLECKAR, V., Z. REHAKOVA, E. BROJEROVA, K. KUCA, D. JUN, K. MACAKOVA, L. OPLETAL, P. DRASAR, L. JAHODAR, J. CHLEBEK, L. CAHLIKOVA. Proanthocyanidins and Their Antioxidation Activity. Chemické Listy, 2012, vol. 106, no. 2, pp. 113-121. IF2011=0.529

NOVAK, Z., J. CHLEBEK, L. OPLETAL, P. JIROS, K. MACAKOVA, J. KUNES,  L. CAHLIKOVA. Corylucinine, a new Alkaloid from Corydalis cava (Fumariaceae), and its Cholinesterase Activity. Natural Product Communications, 2012, vol. 7, no. 7, pp. 859-860. IF2011=1.242

ZATLOUKALOVA L., T. FILIPSKY, P. MLADENKA, V. SEMECKY, K. MACAKOVA, M. HOLECKOVA, J. VAVROVA, V. PALICKA, R. HRDINA. Dexrazoxane provided moderate protection in a catecholamine model of severe cardiotoxicity. Canadian Journal of Physiology and Pharmacology, 2012, vol. 90, no. 4, pp. 473-484. IF2011=1,546


CAHLIKOVA, L., S. ZAVADIL, K. MACAKOVA, I. VALTEROVA, A. KULHANKOVA, A. HOSŤÁLKOVA, J. KUNES, L. OPLETAL. Isolation and Cholinesterase Activity of Amaryllidaceae Alkaloids from Nerine bowdenii. Natural Product Communications, 2011, vol. 6, no. 12, pp. 1827-1830. IF2010=0.894

CAHLIKOVA, L., I. VALTEROVA, K. MACAKOVA, L. OPLETAL. Analysis of Amaryllidaceae alkaloids from Zephyranthes grandiflora by GC/MS and their cholinesterase activity. Revista Brasileira de Farmacognosia-Brazilian Journal of Pharmacognosy, 2011, vol. 21, no. 4, pp. 575-580.

CAHLIKOVA, L., K. MACAKOVA, S. ZAVADIL, P. JIROS, L. OPLETAL, K. URBANOVA, L. JAHODAR. Analysis of Amaryllidaceae Alkaloids from Chlidanthus fragrans by GC-MS and their Cholinesterase Activity. Natural Product Communications, 2011, vol. 6, No. 5, pp. 603-606. IF2010=0.894

CAHLIKOVA, L., N. BENESOVA, K. MACAKOVA, K. URBANOVÁ, L. OPLETAL. GC/MS Analysis of three Amaryllidaceae species and their cholinesterase activity. Natural Product Communications, 2011, vol. 6, no. 9, pp. 1255-1258. IF2010=0.894

CAHLIKOVA, L., K. MACAKOVA, J. CHLEBEK, A. HOSTALKOVA, A. KULHANKOVA, L. OPLETAL. Ecdysterone and its Activity on some Degenerative Diseases. Natural Product Communications, 2011, vol. 6, no. 5, pp. 707-718. IF2010=0.894

FRYDRYCHOVA, S., L. OPLETAL, L., K. MACAKOVA, A. LUSTYKOVA, M. ROZKOT, J. LIPENSKY. Effects of herbal preparation on libido and semen quality in boars. Reproduction in Domestic Animals, 2011, vol. 46, no. 4, pp. 573-578. IF2010=1.606

CHLEBEK, J., K. MACAKOVA, L. CAHLIKOVA, M. KURFURST, J. KUNES, L. OPLETAL. Acetylcholinesterase and butyrylcholinesterase inhibitory compounds from Corydalis cava (Fumariaceae). Natural Product Communications, 2011, vol. 6, no. 5, pp. 607-610. IF2010=0.894

MLADENKA, P., K. MACAKOVA, T. FILIPSKY, L. ZATLOUKALOVA, L. JAHODAR, P. BOVICELLI, P. SILVESTRI, R. HRDINA, L. SASO. In vitro analysis of iron chelating activity of flavonoids. Journal of Inorganic Biochemistry, 2011, vol. 105, pp. 693-701. IF2010=3.317


CAHLIKOVA, L., A. KULHANKOVA, K. URBANOVA, I. VALTEROVA, K. MACAKOVA, J. KUNES. Analysis of Amaryllidaceae alkaloids from Zephyranthes robusta by GC-MS and their cholinesterase activity. Natural Product Communications, 2010, vol. 5, no. 8, pp. 1201-1204. IF2009=0.745

CAHLIKOVA, L., K. MACAKOVA, J. KUNES, M. KURFURST, L. OPLETAL, J. CVACKA, J. CHLEBEK, G. BLUNDEN. Acetylcholinesterase and Butyrylcholinesterase Inhibitory Compounds from Eschscholtzia californica (Papaveraceae). Natural Product Communications, 2010, vol. 5, no. 7, pp. 1035-1038. IF2009=0.745

CAHLIKOVA, L., L. OPLETAL, M. KURFURST, K. MACAKOVA, A. KULHANKOVA, A. HOSTALKOVA. Acetylcholinesterase and Butyrylcholinesterase Inhibitory Compounds from Chelidonium majus (Papaveraceae). Natural Product Communications, 2010, vol. 5, no. 11, pp. 1751-1754. IF2009=0.745

KOLECKAR, V., L. OPLETAL, K. MACAKOVA, L. JAHODAR, D. JUN, J. KUNES, K. KUCA. New Antioxidant Flavonoid Isolated from Leuzea carthamoides. Journal of Enzyme Inhibition and Medicinal Chemistry, 2010, vol. 25, no. 1, pp. 143-145. IF2009=1.496

MACAKOVA, K., L. OPLETAL, M. POLASEK, V. SAMKOVA. Free-radical Scavenging Activity of Some European Polyporales. Natural Product Communications, 2010, vol. 5, no. 6, pp. 923-926. IF2009=0.745

MLADENKA P., K. MACAKOVA, L. ZATLOUKALOVA, Z. REHAKOVA, B.K. SINGH, A.K. PRASAD, V.S. PARMAR, L. JAHODAR, R. HRDINA, L. SASO. In vitro interactions of coumarins with iron. Biochimie, 2010, vol. 92,pp. 1108-1114. IF2009=3.897


JANOVSKA, D, K. KUBIKOVA, L. KOKOŠKA. (2003) Screening for antimicrobial activity of some medicinal plants species of traditional Chinese medicine. Czech Journal of Food Sciences, 2003, vol. 21, no. 3, pp. 107-110.

KOLECKAR, V., E. BROJEROVA., Z. REHAKOVA, K. KUBIKOVA, F. CERVENKA, K. KUCA, D. JUN, M. HRONEK, V. OPLETALOVA, L. OPLETAL. In vitro antiplatelet activity of flavonoids from Leuzea carthamoides. Drug and Chemical Toxicology, 2008, vol. 31, pp. 27-35. IF2007=1.056

KOLECKAR, V., L. OPLETAL, E. BROJEROVA., Z. REHAKOVA, F. CERVENKA, K. KUBIKOVA., K. KUCA, D. JUN, M. POLASEK, J. KUNES, L. JAHODAR.  Evaluation of natural antioxidants of Leuzea carthamoides as a result of screening study of 88 plant extracts from the European Asteraceae and Cichoriaceae. Journal of Enzyme Inhibition and Medicinal Chemistry, 2008, vol. 23, no. 2, pp. 218-224. IF2007=1.343

KOLECKAR, V., K. KUBIKOVA, Z. REHAKOVA, K. KUCA, D. JUN, L. JAHODAR, L. OPLETAL.  Condensed and hydrolysable tannins as antioxidants influencing the health. Mini-Reviews in Medicinal Chemistry, 2008, vol. 8, no. 5, pp. 436-447. IF2007=3.060

MACAKOVA, K., L. OPLETAL, M. POLASEK, V. SAMKOVA, L. JAHODAR. Free-radical scavenging activity of some European Boletales. Natural Product Communications, 2009, vol. 4, no. 2, pp. 261-264. IF2008=0.766

MACAKOVA, K. Expektorancia přírodního původu I. Praktické lékárenství, 2008, vol. 4, no. 4, pp. 193-194.

OPLETAL, L., K. MACAKOVA, L. CAHLIKOVA. Antiinvazní látky přírodního původu jako preventivní faktor v ontogenezi zvířat. In Přírodní látky a jejich biologická aktivita (OPLETAL, L. a V. SKRIVANOVA, eds). Praha: Karolinum, 2010. sv. 2: Využití látek pro ovlivnění fyziologických procesů hospodářských zvířat, s. 203-309, ISBN 978-80-246-1801-2.

STODULKA, P., V. KOLECKAR, D. JUN, K. KUCA, Z. REHAKOVA, K. KUBIKOVA, L. JAHODAR, J. KUNES, L. OPLETAL. High-performance liquid chromatography analysis analysis of four Leuzea carthamoides flavonoids. Journal of Chromatographic Science, 2008, vol. 46, no. 2, pp. 162-164. IF2007=0.869

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