Laboratory of electromigration techniques
Laboratory of electromigration techniques (ELFO lab) is focused on the development and validation of capillary electromigration separation methods (such as capillary zone electrophoresis, CZE) applicable to the assay of biologically active compounds of pharmaceutical or biomedical importance mainly in plant materials (extracts), pharmaceutical formulations and food supplements.
Staff
PharmDr. Pavel Jáč, Ph.D.
Doc. RNDr. Miroslav Polášek, CSc.
Doc. RNDr. Marie Pospíšilová, CSc.
Ph.D. Students:
We are currently looking for new Ph.D. students with the interest in separation sciences, especially in electromigration methods. If you are interested to join our laboratory, don´t hesitate to contact us.
Mgr. Lucie Pincová (now distance study)
Topic of Ph.D. thesis: Development of electromigration methods for the determination of pharmaceutically important substances (Supervisor: Doc. RNDr. Miroslav Polášek, CSc.)
Running diploma projects:
Development of capillary electrophoresis based method for screening of adulterants in weight-loss supplements (Supervisor: Doc. RNDr. Miroslav Polášek, CSc.; co-supervisor: PharmDr. Klára Petrů, Ph.D.)
Testing of potential capability of chiral ionic liquids with long alkyl chain in capillary electrophoresis chiral separations I (Supervisor: PharmDr. Pavel Jáč, Ph.D.; co-supervisor: Doc. RNDr. Miroslav Polášek, CSc.)
Development of CE based assay for the determination of flavonolignans in pharmaceuticals and dietary supplements containing Silymarin. (Supervisor: PharmDr. Pavel Jáč, Ph.D.; co-supervisor: Doc. RNDr. Marie Pospíšilová, CSc.)
Running bachelor projects:
Separation methods for the characterization of negatively charged oligosaccharides – a review. (Supervisor: PharmDr. Pavel Jáč, Ph.D.)
New projects (defense in academic year 2016/2017)
There are 2 topics for bachelor project and 2 topics for diploma project FREE! If you are interested to join our laboratory, don´t hesitate to contact us.
New bachelor projects (defense in academic year 2016/2017):
Capability of LIF and LEDIF detection modes in the determination of amino acids in clinically relevant samples by means of capillary electrophoresis – a review (Supervisor: PharmDr. Pavel Jáč, Ph.D.)
The utilization of separation methods in the determination of boswellic acids – a review (Supervisor: PharmDr. Pavel Jáč, Ph.D.)
New diploma projects (defense in academic year 2016/2017):
Development of capillary electrophoresis methods for the determination of biologically active compounds in dietary supplements II (Supervisor: PharmDr. Pavel Jáč, Ph.D.; co-supervisor: Doc. RNDr. Marie Pospíšilová, CSc.)
Testing of potential capability of chiral ionic liquids with long alkyl chain in capillary electrophoresis chiral separations II (Supervisor: PharmDr. Pavel Jáč, Ph.D.; co-supervisor: Doc. RNDr. Miroslav Polášek, CSc.)
Laboratory equipment:
•Agilent 7100 CE Coupled with Diode Array UV/VIS and C4D Detectors (Agilent)
•Beckman P/ACE™ MDQ CE with Diode Array Detector (Beckman Coulter)
•Electrophoretic Analyzer PrinCE-C 750 Coupled with Diode Array UV/VIS (PrinCE Technologies) and C4D (TraceDec) Detectors
•EA 202A with Contactless Conductivity and UV/VIS Detectors - ITP-CZE (Villa Labeco)
Collaborations:
International collaborations
• Prof. Ana M. García Campaña - Department of Analytical Chemistry, University of Granada, Spain – Capillary electrophoresis with MS detection in the analysis of pharmaceutical formulations
• Prof. Marina F. M. Tavares - Institute of Chemistry, University of Sao Paulo, Brazil – Capillary electrophoretic methods in the analysis of illicit drugs
National collaborations
PharmDr. Marcel Špulák, Ph.D. (Department of Inorganic and Organic Chemistry, Faculty of Pharmacy, Hradec Králové) – Synthesis and evaluation of chiral ionic liquids with long alkyl chain in chiral capillary electrophoresis separations
RNDr. Lenka Kujovská Krčmová, Ph.D. (Research laboratory at 3rd Internal Gerontometabolic Clinic, University Hospital Hradec Králové) – Development of CE-assays for biomarkers determination
Assoc. Prof. Jiřina Spilková, CSc. -Department of Pharmacognosy, Faculty of Pharmacy, Hradec Králové - CE analysis of plant extracts