Equipment and methods

Group of pathology and pharmacology of cardiovascular and metabolic disorders

In vivo

Current mice models:

• Transgenic overexpressing human Endoglin mice
• Transgenic Leiden apolipoprotein E3 deficient mice

In vitro

Types of cells:

• Human umbilical vein endothelial cells
• Human aortic endothelial cells
• Human coronary artery endothelial cells (healthy and from diabetes mellitus type II patients)
• Hepatic stellate cells 
• Liver sinusoidal endothelial cells
• THP-monocytes

Methods

• Histology and microscopy

• Light and fluorescence immunohistochemistry

• Western blot analysis and ELISA analysis

• Wire Myograph - functional analysis of blood vessels

• Flow cytometry and in vitro study