PharmDr. Anna Paraskevopoulou, Ph.D.

Research interests

• Models of healthy and damaged skin barrier
  • Langmuir monolayers and lipid films
  • 3D cell culture
  • ex vivo skin explants
• (Trans)dermal drug delivery
  • formulation of drug delivery systems
  • permeability studies
• Skin barrier lipids analysis:
  • High Performance Thin Layer Chromatography (HPTLC)
  • Infrared spectroscopy
  • Raman spectroscopy

Education

• Faculty of Pharmacy in Hradec Králové, Charles University
  • PhD in Pharmaceutical technology (2016-2021)
  • Msc in Pharmacy (2011-2016)

Teaching

• Pharmaceutical Technology I and II
• Medical Devices
• Supervisor of Master and Erasmus students

Publication activity

• (co)-author of 11 peer reviewed publications
  • ∑IFs>60; 193 citations without self-citations; H-index 7
  • Complete list of publication available on ORCID
• Selected publications:
  • Dwivedi A, Mazumder A, Pullmannová P, Paraskevopoulou A, Opálka L, Kováčik A, Macháček M, Jančálková P, Svačinová P, Peterlik H, Maixner J, Vávrová K. Lipid Monolayer on Cell Surface Protein Templates Functional Extracellular Lipid Assembly. Small. 2024, 20, 2307793. IF2023=12.1 (Q1/D1), AIS2023=2.245 (Q1)
  • Paraskevopoulos G, Fandrei F, Pratihast KA, Paraskevopoulou A, Panoutsopoulou E, Opálka L, Singh Mithu V, Huster D, Vávrová K. Effects of imidazolium ionic liquids on skin barrier lipids – Perspectives for drug delivery. J. Colloid Interface Sci. 2024, 659: 449-462. IF2024=9.7 (Q1), AIS2023=1.256 (Q2)
  • Hergesell K, Paraskevopoulou A, Opálka L, Velebný V, Vávrová K, Dolečková I. The effect of long-term cigarette smoking on selected skin barrier proteins and lipids. Sci. Rep. 2023, 13 (1):11572. IF2023=4.6 (Q2), AIS2023=1.129 (Q2)
  • Nováčková A, Sagrafena I, Pullmannová P, Paraskevopoulos G, Dwivedi A, Mazumder A, Růžičková K, Slepička P, Zbytovská J, Vávrová K. Acidic pH is Required for the Multilamellar Assembly of Skin Barrier Lipids in Vitro. J. Invest. Dermatol. 2021, 141(8):1915-1921. IF2021=7.6 (Q1), AIS2021=2.275 (Q1/D1)
  • Jevtić M, Löwa A, Nováčková A, Kováčik A, Kaessmeyer S, Erdmann G, Vávrová K, Hedtrich S. Impact of Intercellular Crosstalk Between Epidermal Keratinocytes and Dermal Fibroblasts on Skin Homeostasis. BBA-Mol. Cell Res. 2020, 1867(8):118722. IF2020=4.7 (Q2), AIS2020=1.484 (Q2)

Grants

• Principal researcher of grant awarded by Charles University: "Glucocorticoid-Mediated Remodeling of Skin Lipid Microstructure and Barrier Integrity" Returns at the CU RTNS25-032 (2026-today)
• Co-worker of 6 grants awarded by Czech Science Foundation, e.g.:
  • 2025-today "Corneocyte lipid envelope: lipid-protein binding mechanisms and barrier rescue in ichthyosis models" GAČR 24-10736S
  • 2022-2025 "Novel Insights into the Nanostructure of the Skin Lipid Barrier by Biophysical Methods" GAČR-DFG 22-20839K
  • 2019-2021 "Ultralong chain ceramides in membrane models of healthy and diseased skin barrier" GAČR-DFG 19-09135J; and "Integrated design methodology of nanoformulation processes for (trans)dermal delivery of actives" GAČR 19-09600S
• Principal researcher of grant awarded by Charles University: "Modulátory bariérové funkce kůže" GAUK 184217 (2017-2019)
• Co-worker of other 3 grants awarded by Charles University: GAUK 1156120 (2020), GAUK 332119 (2019-2021), GAUK 936216 (2016-2018)

Internship

• 3 months in UDEAR Group INSERM, France – supervisor assoc. prof. Nathalie Jonca, PhD (cultivation of reconstructed human epidermis models of Autosomal Recessive Congenital Ichthyoses, application of formulations for skin barrier regeneration)

Selected presentations at international conferences

• Skin Forum 2022 Annual Meeting, Sweden
• Skin and Formulation & 17th Skin Forum, France, 2019 – poster award
• Skin Forum: Skin Health and Topical Formulation, Estonia, 2018
• Gordon Research Conferences, Barrier Function of Mammalian Skin, USA, 2017
• Meeting of the European Society for Dermatological Research, Germany, 2016