Equipment and Methods
|
|
|
|
A vacuum evaporator is used for preparation of summary ethanolic plant extracts in a preparative measurement. |
Further, flash chromatography (Sepacore X10 system; Buchi) with UV and ELS detectors is used for purification of summary alkaloidal plant extracts and obtained fractions. |
|
|
|
|
Furthermore, smaller fractions are fractionated by flash chromatography using puriFlash® 535-XL. |
Preparative HPLC-PDA-MS (Waters) for separation of compounds from fractions. HPLC is also possible used for identification and determination of purity of isolated compounds or screening of plant extracts. |
|
|
|
|
Semi-preparative HPLC for alkaloid mixture separations in several mg. |
Optical rotation of pure and structurally determined alkaloids with chiral centres is measured with an automatic polarimeter. |
|
|
|
|
HPTLC is used for screening of plant extracts/fractions and allows also semi-quantitative determination of compounds and screening of biological activities of metabolites in extracts. |
GC-MS for the determination of a profile of some plant extract alkaloids. |
 |
 |
| Reader Synergy serves for in vitro biological assays (enzymes). |
Synergy Neo2 Multi-Mode Reader in vitro biological assays (enzymes, cell cultures). |
 |
|
| A laminar box for evaluation of cytotoxic activity of isolated alkaloids. |
