The Department is sufficiently equipped for preparation of subcellular fractions (cooling box, homogenizer, centrifuges, ultracentrifuges), for cultivation of cell lines, primary cultures of hepatocytes, and precision-cut tissue slices (tissue slicer, autoclave, laminar box, CO2 and CO2/O2 incubators), for enzyme activities assays (thermomixers, spectrophotometers, spectrofluorimeters, HPLC), for immunodetection of proteins (electrophoresis, blotting equipment), for study of biotransformation of xenobiotics and detection, identification and quantification of metabolites (HPLC, UHPLC-MS/MS) and for molecular diagnostic techniques (equipment for PCR, RT-PCR, electrophoresis).
Important equipment
Krumdieck Tissue Slicer - Model MD4000 - Details
Real-Time PCR System - Details
Real-Time Cell Analyzer - Roche xCELLigence RTCA DP
Homogenizers
- Potter S Homogenizer – B. Braun Biotech International
- SONOPULS HD 2070 Ultrasonic homogenizer – Bandelin
- FastPrep 24TM 5G – MP Biomedicals - Details
- Homogenizer HG-24 – Biobase - Details
Mass spectrometers
Mass spectrometer LCQ Advantage (3D ion trap), ESI and APCI ionization techniques (installed 2001) – Thermo Scientific
Mass spectrometer LCMS-8030 Triple Quadrupole Liquid Chromatograph Mass Spectrometer (LC-MS/MS) (installed 2012) - Shimadzu
HPLC
- HPLC system Agilent 1260 Infinity II – Agilent
- UHPLC system Agilent 1290
- HPLC system Agilent – 1200 RR
- Shimadzu - 10AVP
- Thermo Scientific – Surveyor
Other equipment
- Eppendorf thermomixer
- Eppendorf Concentrator 5301
- Heraeus Biofuge Stratos Centrifuges
- Beckman coulter Ultracentrifuge – AVANTI J-30I
- Sorvall Ultracentrifuges WX 80, OTD Combi
- CO2 Incubators
- Hera cell
- Forma scientific
- Memmert ICO240med
- CO2/O2 Incubator - Binder CB-60
- AURA VERTICAL SD4, Aura 2000 M.A.C - microbiological safety cabinet
- Three freezers -80 °C (ARCTICO, NEW BRUNSWICK)