Equipment

The Department is sufficiently equipped for preparation of subcellular fractions (cooling box, homogenizer, centrifuges, ultracentrifuges), for cultivation of cell lines, primary cultures of hepatocytes, and precision-cut tissue slices (tissue slicer, autoclave, laminar box, CO2 and CO2/O2 incubators), for enzyme activities assays (thermomixers, spectrophotometers, spectrofluorimeters, HPLC), for immunodetection of proteins (electrophoresis, blotting equipment), for study of biotransformation of xenobiotics and detection, identification and quantification of metabolites (HPLC, UHPLC-MS/MS) and for molecular diagnostic techniques (equipment for PCR, RT-PCR, electrophoresis). 

Important equipment 

Krumdieck Tissue Slicer - Model MD4000 - Details
Tissue slicer

Real-Time PCR System - Details

Real-Time Cell Analyzer - Roche xCELLigence RTCA DP
P2157690.JPG

Homogenizers​

  • Potter S Homogenizer – B. Braun Biotech International 
  • SONOPULS HD 2070 Ultrasonic homogenizer – Bandelin
  • FastPrep 24TM 5G – MP Biomedicals - Details
  • Homogenizer HG-24 – Biobase - Details

Mass spectrometers
Mass spectrometer LCQ Advantage (3D ion trap), ESI and APCI ionization techniques (installed 2001) – Thermo Scientific
LC/MS

Mass spectrometer LCMS-8030 Triple Quadrupole Liquid Chromatograph Mass Spectrometer (LC-MS/MS) (installed 2012) - Shimadzu

HPLC

  • HPLC system Agilent 1260 Infinity II – Agilent
  • UHPLC system Agilent 1290
  • HPLC system Agilent – 1200 RR
  • Shimadzu - 10AVP
  • Thermo Scientific – Surveyor

Other equipment

  • Eppendorf thermomixer
  • Eppendorf Concentrator 5301
  • Heraeus Biofuge Stratos Centrifuges
  • Beckman coulter Ultracentrifuge – AVANTI J-30I
  • Sorvall Ultracentrifuges WX 80, OTD Combi
  • CO2 Incubators
    • Hera cell
    • Forma scientific
    • Memmert ICO240med
  • CO2/O2 Incubator - Binder CB-60
  • AURA VERTICAL SD4, Aura 2000 M.A.C - microbiological safety cabinet
  • Three freezers -80 °C (ARCTICO, NEW BRUNSWICK)

 

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