Current position
Education and Employment
- Since 2014 Associate Professor at the Department of Biochemical Sciences, Charles University in Prague, Faculty of Pharmacy in Hradec Králové, subject: Biochemistry
- 2006-2014 Assistant Professor at the Department of Biochemical Sciences, Faculty of Pharmacy in Hradec Králové, Charles University
- 2006 Ph.D., Charles University in Prague, Faculty of Pharmacy in Hradec Králové, studying program: Pharmacy, subject: Toxicology of Natural Compounds
- 2002 MSc., Charles University in Prague, Faculty of Pharmacy in Hradec Králové, studying program: Pharmacy
Research fellowship
- 2005-2006 research fellowship at the Institute for Environmental Medicine, Medical School, University of Pennsylvania, Philadelphia, USA (6 months)
- 2004 research fellowship at the Department of Human Physiology and Pharmacology “Vittorio Erspamer”, University of Rome “La Sapieza”, Rome, Italy (5 months)
Research Interest
- Changes in the expression and activity of drug-metabolizing and antioxidant enzymes during ageing and under pathological states (e.g. non-alcoholic fatty liver disease, diabetes mellitus, obesity) in the in vitro as well as in vivo models
- Biological activity of low-molecular compounds with antioxidant/pro-oxidant properties (e.g. flavonoids, monoterpenes, sesquiterpenes)
- Influence of naturally occurring plant secondary metabolites on the mechanisms of epigenetic regulation
Teaching experience
- Clinical Biochemistry and Pathobiochemistry
- Pathobiochemistry
- Supervisor of bachelor, diploma, and rigorous thesis students (>40 finished, 8 ongoing)
- Supervisor/supervisor specialist of postgraduate students (2 graduated, 4 ongoing)
Professional Memberships
- Czech Society for Biochemistry and Molecular Biology (CSBMB)
- Czech Society of Clinical Biochemistry (CSKB)
- Czech Medical Association of J. E. Purkyně (CLS JEP)
Scientific Grants
Czech Science Foundation
- 2009-2011 GAČR 524/09/P121 (principal investigator) - Modification of enzymes metabolizing xenobiotics during ageing
- 2012-2018 GAČR P303/12/G163 (team member) - Centre of drug-dietary supplements interactions and nutrigenetics
- 2018-2021 GAČR 18-09946S (principal investigator) - Metabolism of terpenes and mechanisms of their toxic effects in human liver
Grant Agency of Charles University, University Research Centre
- 2000-2002 GAUK 95/2000 (team member)
- 2005-2006 GAUK 128/2005 (team member) - Účinky antioxidantů přírodního původu v enzymovém modelu glykoxidace proteinů in vitro.
- 2012-2014 UNCE 204026/2012 (junior researcher) - Study of Drugs and Other Biologically-Active Substances Perspective in Prevention and Treatment of Important Lifestyle Diseases
- 2014-2016 GAUK 296314 (team member) - Studium antiproliferační a pro-oxidační aktivity seskviterpenů a jejich potenciální interakce s cytostatiky
- 2020-2022 GAUK 1302120 (supervisor of PI) - The investigation of biotransformation of reactive sesquiterpenes and their effect on the proteome of human hepatic cells
- 2021-2023 GAUK 240121 (supervisor of PI) - Changes in the expression and activities of detoxification enzymes in non-alcoholic fatty liver disease
- 2021-2023 START/MED/065 (mentor) - Novel therapeutic approaches to the treatment of hepatic diseases
- 2022-2024 GAUK 318422 (supervisor of PI) - Epigenetic changes in progression of non-alcoholic fatty liver disease
Publications
- Number of publications: 47
- Number of citations: >1400 (WoS; excluding self-citation)
- H-index: 16 (WoS)
- Overall impact factor: >137
Most of the papers with full-text links can be found with the PubMed Search “Bousova I”
Publications over the last five years:
Dimunová D., Matoušková P., Podlipná R., Boušová I., Skálová L. (2022) The role of UDP-glycosyltransferases in xenobiotic-resistance. Drug Metab. Rev. in press.
Kurtzeborn K., Kwon H.N., Iaroshenko V., Faisal I., Ambrož M., Jin X., Qureshi T., Kupari J., Ihermann-Hella A., Väänänen J., Tyynismaa H., Boušová I., Park S., Kuure S. (2022) Comparative whole-genome transcriptome analysis in renal cell populations reveals high tissue specifcity of MAPK/ERK targets in embryonic kidney. BMC Biol. 20: 112.
Šadibolová M., Juvonen R.O., Auriola S., Boušová I.* (2022) In vitro metabolism of helenalin and its inhibitory effect on human cytochrome P450 activity. Arch. Toxicol. 96: 793–808.
Hanousková B., Vávrová G., Ambrož M., Boušová I., Karlsen T.A., Skálová L., Matoušková P. (2021) MicroRNAs mediated regulation of glutathione peroxidase 7 expression and its changes during adipogenesis. Biochim. Biophys. Acta Gene Regul. Mech.1864: 194734.
Lněničková K., Šadibolová M., Matoušková P., Szotáková B., Skálová L., Boušová I.* (2020) The modulation of phase II drug-metabolizing enzymes in proliferating and differentiated CaCo-2 cells by hop-derived prenylflavonoids. Nutrients. 12: 2138.
Zárybnický T., Matoušková P., Skálová L., Boušová I.* (2020) The hepatotoxicity of alantolactone and germacrone: their influence on cholesterol and lipid metabolism in differentiated HepaRG cells. Nutrients. 12: 1720.
Zárybnický T., Matoušková P., Ambrož M., Šubrt Z., Skálová L., Boušová I.* (2019) The selection and validation of reference genes for mRNA and microRNA expression studies in human liver slices using RT-qPCR. Genes. 10: 763.
Šadibolová M., Zárybnický T., Smutný T., Pávek P., Šubrt Z., Matoušková P., Skálová L., Boušová I.* (2019) Sesquiterpenes Are Agonists of the Pregnane X Receptor but Do Not Induce the Expression of Phase I Drug-Metabolizing Enzymes in the Human Liver. Int. J. Mol. Sci. 20: 4562.
Ambrož M., Lněničková K., Matoušková P., Skálová L., Boušová I.* (2019) Antiproliferative effects of hop-derived prenylflavonoids and their influence on the efficacy of oxaliplatine, 5-fluorouracil and irinotecan in human colorectalc cells. Nutrients 11: 879.
Zárybnický T., Matoušková P., Lancošová B., Šubrt Z., Skálová L., Boušová I.* (2018) Inter-individual variability in acute toxicity of R-pulegone and R-menthofuran in human liver slices and their influence on miRNA expression changes in comparison to acetaminophen. Int. J. Mol. Sci. 19: 1805.
Zárybnický T., Boušová I., Ambrož M., Skálová L. (2018) Hepatotoxicity of monoterpenes and sesquiterpenes. Arch. Toxicol. 92: 1-13.