Assoc. Prof. PharmDr. Iva Boušová, Ph.D.

Current position

Education and Employment

  • Since 2014 Associate Professor at the Department of Biochemical Sciences, Charles University in Prague, Faculty of Pharmacy in Hradec Králové, subject: Biochemistry
  • 2006-2014 Assistant Professor at the Department of Biochemical Sciences, Faculty of Pharmacy in Hradec Králové, Charles University
  • 2006 Ph.D., Charles University in Prague, Faculty of Pharmacy in Hradec Králové, studying program: Pharmacy, subject: Toxicology of Natural Compounds
  • 2002 MSc., Charles University in Prague, Faculty of Pharmacy in Hradec Králové, studying program: Pharmacy

Research fellowship

  • 2005-2006 research fellowship at the Institute for Environmental Medicine, Medical School, University of Pennsylvania, Philadelphia, USA (6 months)
  • 2004 research fellowship at the Department of Human Physiology and Pharmacology “Vittorio Erspamer”, University of Rome “La Sapieza”, Rome, Italy (5 months)

Research Interest

  • Changes in the expression and activity of drug-metabolizing and antioxidant enzymes during ageing and under pathological states (e.g. non-alcoholic fatty liver disease, diabetes mellitus, obesity) in the in vitro as well as in vivo models
  • Biological activity of low-molecular compounds with antioxidant/pro-oxidant properties (e.g. flavonoids, monoterpenes, sesquiterpenes)
  • Influence of naturally occurring plant secondary metabolites on the mechanisms of epigenetic regulation

Teaching experience

  • Clinical Biochemistry and Pathobiochemistry
  • Pathobiochemistry
  • Supervisor of bachelor, diploma, and rigorous thesis students (>40 finished, 8 ongoing)
  • Supervisor/supervisor specialist of postgraduate students (2 graduated, 4 ongoing)

Professional Memberships

  • Czech Society for Biochemistry and Molecular Biology (CSBMB)
  • Czech Society of Clinical Biochemistry (CSKB)
  • Czech Medical Association of J. E. Purkyně (CLS JEP)

Scientific Grants

Czech Science Foundation

  • 2009-2011 GAČR 524/09/P121 (principal investigator) - Modification of enzymes metabolizing xenobiotics during ageing          
  • 2012-2018 GAČR P303/12/G163 (team member) - Centre of drug-dietary supplements interactions and nutrigenetics          
  • 2018-2021 GAČR 18-09946S (principal investigator) - Metabolism of terpenes and mechanisms of their toxic effects in human liver

Grant Agency of Charles University, University Research Centre

  • 2000-2002 GAUK 95/2000 (team member)
  • 2005-2006 GAUK 128/2005 (team member) - Účinky antioxidantů přírodního původu v enzymovém modelu glykoxidace proteinů in vitro.
  • 2012-2014 UNCE 204026/2012 (junior researcher) - Study of Drugs and Other Biologically-Active Substances Perspective in Prevention and Treatment of Important Lifestyle Diseases
  • 2014-2016 GAUK 296314 (team member) - Studium antiproliferační a pro-oxidační aktivity seskviterpenů a jejich potenciální interakce s cytostatiky
  • 2020-2022 GAUK 1302120 (supervisor of PI) - The investigation of biotransformation of reactive sesquiterpenes and their effect on the proteome of human hepatic cells
  • 2021-2023 GAUK 240121 (supervisor of PI) - Changes in the expression and activities of detoxification enzymes in non-alcoholic fatty liver disease
  • 2021-2023 START/MED/065 (mentor) - Novel therapeutic approaches to the treatment of hepatic diseases
  • 2022-2024 GAUK 318422 (supervisor of PI) - Epigenetic changes in progression of non-alcoholic fatty liver disease


  • Number of publications: 47
  • Number of citations: >1400 (WoS; excluding self-citation)
  • H-index: 16 (WoS)
  • Overall impact factor: >137

Most of the papers with full-text links can be found with the PubMed Search “Bousova I”

Publications over the last five years:

Dimunová D., Matoušková P., Podlipná R., Boušová I., Skálová L. (2022) The role of UDP-glycosyltransferases in xenobiotic-resistance. Drug Metab. Rev. in press.

Kurtzeborn K., Kwon H.N., Iaroshenko V., Faisal I., Ambrož M., Jin X., Qureshi T., Kupari J., Ihermann-Hella A., Väänänen J., Tyynismaa H., Boušová I., Park S., Kuure S. (2022) Comparative whole-genome transcriptome analysis in renal cell populations reveals high tissue specifcity of MAPK/ERK targets in embryonic kidney. BMC Biol. 20: 112.

Šadibolová M., Juvonen R.O., Auriola S., Boušová I.* (2022) In vitro metabolism of helenalin and its inhibitory effect on human cytochrome P450 activity. Arch. Toxicol. 96: 793–808.

Hanousková B., Vávrová G., Ambrož M., Boušová I., Karlsen T.A., Skálová L., Matoušková P. (2021) MicroRNAs mediated regulation of glutathione peroxidase 7 expression and its changes during adipogenesis. Biochim. Biophys. Acta Gene Regul. Mech.1864: 194734.

Lněničková K., Šadibolová M., Matoušková P., Szotáková B., Skálová L., Boušová I.* (2020) The modulation of phase II drug-metabolizing enzymes in proliferating and differentiated CaCo-2 cells by hop-derived prenylflavonoids. Nutrients. 12: 2138.

Zárybnický T., Matoušková P., Skálová L., Boušová I.* (2020) The hepatotoxicity of alantolactone and germacrone: their influence on cholesterol and lipid metabolism in differentiated HepaRG cells. Nutrients. 12: 1720.

Zárybnický T., Matoušková P., Ambrož M., Šubrt Z., Skálová L., Boušová I.* (2019) The selection and validation of reference genes for mRNA and microRNA expression studies in human liver slices using RT-qPCR. Genes. 10: 763.

Šadibolová M., Zárybnický T., Smutný T., Pávek P., Šubrt Z., Matoušková P., Skálová L., Boušová I.* (2019) Sesquiterpenes Are Agonists of the Pregnane X Receptor but Do Not Induce the Expression of Phase I Drug-Metabolizing Enzymes in the Human Liver. Int. J. Mol. Sci. 20: 4562.

Ambrož M., Lněničková K., Matoušková P., Skálová L., Boušová I.* (2019) Antiproliferative effects of hop-derived prenylflavonoids and their influence on the efficacy of oxaliplatine, 5-fluorouracil and irinotecan in human colorectalc cells. Nutrients 11: 879.

Zárybnický T., Matoušková P., Lancošová B., Šubrt Z., Skálová L., Boušová I.* (2018) Inter-individual variability in acute toxicity of R-pulegone and R-menthofuran in human liver slices and their influence on miRNA expression changes in comparison to acetaminophen. Int. J. Mol. Sci. 19: 1805.

Zárybnický T., Boušová I., Ambrož M., Skálová L. (2018) Hepatotoxicity of monoterpenes and sesquiterpenes. Arch. Toxicol. 92: 1-13.

© Charles University, Faculty of Pharmacy in Hradec Králové, Akademika Heyrovského 1203, 500 03 Hradec Králové, Czech Republic
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