Mgr. Ondřej Horáček, Ph.D.

Present position

• Teaching Assistant at the Department of Pharmaceutical Chemistry and Pharmaceutical Analysis, Faculty of Pharmacy in Hradec Králové, Charles University
• Member of the Pharmaceutical Analysis Group

Research interests

• Liquid chromatography, gas chroamtography, supercritical fluid chromatography, capillary electrophoresis
• Development of analytical and bioanalytical methods (HPLC/UHPLC-MS/MS; GC, GC-MS)
• Possibility of employment of different materials for sample preparation
• Chiral separations of biologically active compounds
• Analysis of boron cluster compounds

Education and professional carrier

Ph.D., Pharmaceutical Analysis, Charles University, Faculty of Pharmacy (2023)

Focus: Pharmaceutical Analysis, Chiral Separation, Separation Sciences, Analytical Chemistry
Dissertation title: Chiral Separation of Boron Cluster Compounds
Supervisor: doc. PharmDr. Radim Kučera, Ph.D.

Mgr., Medical Bioanalytics, Charles University, Faculty of Pharmacy (2018)
Focus: Bioanalytical Chemistry
Master thesis title: Achiral and chiral high-performance liquid chromatography of drugs and their metabolites III.
Supervisor: prof. PharmDr. Milan Nobilis, CSc.

Teaching experience

Teaching Assistant, Department of Pharmaceutical Chemistry and Pharmaceutical Analysis, Faculty of Pharmacy, Charles University (2019-present)
Courses: Pharamceutical Analysis I and II in Czech and English language
Job description: Practical classes and Seminars

Supervisor of 1 and co-supervisor of 6 M.Sc. theses

Continuing education and other experience

Course "Science and AI: Partnership for Future" at UHK (2024)
Programme of Lifelong learning at Charles University "Výsledky učení" (2024)
Chairman of the session Selective separations at conference HPLC 2022 in San Diego (2022)
Programme of Lifelong learning at Charles University "Vzdělávací program pedagogických dovedností" (2021)

Grants received

Principal investigator:

2020-2023 Grant Agency of Charles University: Analytical study of the influence of experimental conditions on the chiral separation of boron cluster compounds. (GAUK No. 168120)

Member of the research team:

2020-2024 - Specialized Team for Advanced Research on Separation Science (STARSS), Registration number of project: CZ.02.1.01/0.0/0.0/15_003/0000465

Internships

01/2022–07/2022 University of Texas at Arlington, research group prof. D.W. Armstrong
Content of the internship:
Chiral separations of boron cluster compounds on chiral stationary phases immobilized on superficially porous particles.
Development of comprehensive chiral methods using GC-MS/MS and LC-MS/MS for identification and quantitation of N-acyl homoserine lactones in bacterial media.

Professional memberships

Czech Society of Mass Spectrometry

Czech Pharmaceutical Society

Cooperations

Faculty of Pharmacy in Hradec Kralove, Charles University
Prof. Lucie Nováková, Ph.D. - Department of Analytical Chemistry – Supercritical fluid chromatography

The Czech Academy of Science
Dr. Bohumír Grüner, Ph.D. (Institute of Inorganic Chemistry, Řež) - chiral separations of born cluster compounds.

The University of Texas at Arlington
Prof. Daniel W. Armstrong, Ph. D. – Department of Chemistry and Biochemistry – Chiral separations of homoserine lactones, Chiral separations of boron cluster compounds

Conferences

Oral presentations at 5 local international conferences and 1 local conference.
Poster presentations at 4 international conferences abroad and 2 local international conferences.

Publications

Author and co-author of 11 articles in international journals with IF; 48 citations (Scopus); H‑index: 5 (Scopus). The links to full texts are available at my ResearchGate profile.

2023

Horáček O., Portillo A.E., Dhaubhadel U., Sung Y., Readel E.R., Kučera R., Armstrong D.W. Comprehensive chiral GC-MS/MS and LC-MS/MS methods for identification and determination of N-acyl homoserine lactones. Talanta. 2023; 253: 123957. (IF₂₀₂₂: 6.1, Q1; AIS₂₀₂₂: 0.793, Q1)

Author’s contribution: investigation, data curation, writing original draft and editing, visualization.

Horáček O., Dhaubhadel U., Holub J., Grüner B., Arstrong D.W., Kučera R. Employment of chiral columns with superficially porous particles in chiral separations of cobalt bis(dicarbollide) and nido-7,8-C₂B₉H₁₂(1-) derivatives. Chirality. https://doi.org/10.1002/chir.23606 [published online]. (IF₂₀₂₂: 2.0, Q3; AIS₂₀₂₂: 0.354, Q3)

Author’s contribution: investigation, data curation, conceptualization, methodology, writing original draft and editing, visualization.

Portillo A.E., Dhaubhadel U., Horáček O., Sung Y., Armstrong D.W. Investigating chirality in quorum sensing by analysis of Burkholderia cepacia and Vibrio fischeri with comprehensive chiral LC-MS/MS and GC-MS/MS methods. FEMS Microbiology Letters. 2023; 370: fnad011. (IF₂₀₂₂: 2.1, Q4; AIS₂₀₂₂: 0.582, Q3)

Author’s contribution: investigation, data curation, original draft editing.

Dhaubhadel U., Portillo A.E., Horáček O., Sung Y., Armstrong D.W. Unusual enantiomeric D,L-N-acyl homoserine lactones in Pectobacterium atrosepticum and Pseudomonas aeruginosa PLoS ONE. 2023; 18(3): 1. (IF₂₀₂₂: 3.7, Q2; AIS₂₀₂₂: 0.944, Q2)

Author’s contribution: investigation, data curation, original draft editing.

Khalikova M., Jireš J., Horáček O., Douša M., Kučera R., Nováková L.  What is the role of current mass spectrometryin pharmaceutical analysis? Mass Spectrometry Reviews. 2023; : 1-50. (IF₂₀₂₂: 6.6, D1; AIS₂₀₂₂: 1.862, Q1)

Author’s contribution: writing (sections: 2.2 MS in Pharmacopoeia  2.5 The role of MS in determination of pharmaceuticals in biological fluids) and editing original draft, proofreading

Plachká K., Pilařová V., Horáček O., Gazárková T., Kočová Vlčková H., Kučera R., Nováková L. Columns in analytical-scale supercritical fluid chromatography: From traditional to unconventional chemistries. Journal of Separation Science (Accepted for Publication). (IF₂₀₂₂: 3.1, Q2; AIS₂₀₂₂: 0.371, Q3)

Author’s contribution: comprehensive literature search, writing (section: 3. Chiral stationary phases in SFC) and editing original draft, proofreading

2022

Horáček O., Nováková L., Tüzün E., Grüner B., Švec F., Kučera R. Advanced Tool for Chiral Separations of Anionic and Zwitterionic (Metalla)carboranes: Supercritical Fluid Chromatography. Analytical Chemistry. 2022; 94: 17551-17558. (IF₂₀₂₂: 7.4, D1; AIS₂₀₂₂: 1.303, Q1)

Author’s contribution: investigation, data curation, conceptualization, methodology, writing original draft and editing, visualization.

Horáček O., Marvalová J., Štilcová K., Holub J., Grüner B., Kučera R. Reversed-phase chromatography as an effective tool for the chiral separation of anionic and zwitterionic carboranes using polysaccharide-based chiral selectors. Journal of Chromatography A. 2022; 1672: 463051. (IF₂₀₂₂: 4.1, Q2; AIS₂₀₂₂: 0.553, Q2)

Author’s contribution: investigation, data curation, conceptualization, methodology, funding acquisition, writing original draft and editing, visualization.

El Anwar S., Pazderová L., Bavol D., Bakardjiev M., Ruzickova Z., Horáček O., Fojt L., Kucera R., Gruner B. Structurally rigidified cobalt bis(dicarbollide) derivatives, a chiral platform for labelling of biomolecules and new materials. Chemical Communications. 2022; 58: 2572–2575. (IF₂₀₂₂: 4.9, Q2; AIS₂₀₂₂: 1.031, Q2)

Author’s contribution: investigation – HPLC chiral separations of oxygen-bridged cobalt bis(dicarbollide) derivatives, data curation, writing the analytical part, visualization.

2021

Horáček O., Papajová-Janetková M., Grüner B., Lochman L., Štěrbová-Kovaříková P., Vespalec R., Kučera R. The first chiral HPLC separation of dicarba-nido-undecarborate anions and their chromatographic behavior. Talanta. 2021; 222: 121652. (IF₂₀₂₁: 6.556, Q1; AIS_2021: 0.765, Q1)

Author’s contribution: investigation, data curation, methodology, writing original draft and editing, visualization.

2020

Juhás M., Kučerová M., Horáček O., Janďourek O., Kubíček V., Konečná K., Kučera R., Bárta P., Janoušek J., Paterová P., Kuneš J., Doležal M., Zitko J. N-Pyrazinoyl Substituted Amino Acids as Potential Antimycobacterial Agents-The Synthesis and Biological Evaluation of Enantiomers. Molecules. 2020; 25(7): 1-29. (IF₂₀₂₀: 4.412, Q2; AIS₂₀₂₀: 0.694, Q2)

Author’s contribution: investigation – HPLC chiral separations of N-pyrazinoyl substituted amino acids, data curation, writing the analytical part, visualization.