RNDr. Miloslav Macháček, Ph.D.

Date and Place of Birth: 7 December 1987, Prague, Czech Republic

Present Position:

Contact Information:

  • Work Address: Akademika Heyrovského 1203/8, 500 05 Hradec Králové, Czech Republic
  • E-mail: machamil@faf.cuni.cz
  • Tel.: +420 495 067 588

Personal Identifiers:

Research Interests

  • photodynamic therapy of cancer
  • mode of cellular death
  • oxidative stress on cellular and molecular level
  • evaluation of toxicological and pharmacological properties of novel phthalocyanine and azaphthalocyanine (tetrapyrazinoporphyrazine and tetrapyridoporphyrazine) photosensitizers
  • fluorescence and confocal microscopy
     
  • list of publications , see below

Grant projects

  • principal investigator, Czech Science Foundation, GAČR 19-14758Y, “Novel photosensitizers for vascular targeted photodynamic therapy.” (2019-2021)
  • principal investigator, Grant Agency of Charles University, GAUK 1916214, “Study of the novel phthalocyanine and azaphthalocyanine photosensitizers for photodynamic therapy of tumors on cellular and molecular level.” (2014-2016, evaluation: "excellent")
  • member of the team in other research grants and projects:
    5× Czech Science Foundation - GAČR 19-09600S, GAČR 18-08169S, 13-27761S, 13-15008S, 13-23891S
    1× University Centre of Excellence - UNCE 204019/304019/2012
    3× Grant Agency of Charles University - GAUK 367911, GAUK 1404213, GAUK 88615
    1× Grant Agency of Charles University (as a supervisor) - GAUK 1620219

Professional membership

Educational activities

  • supervisor of 1 disertation thesis (since 2018)
  • supervisor of 9 B.Sc. and 13 M.Sc. (Pharmacy and Medical Bioanalytics) diploma theses (since 2014)
  • Pathological Biochemistry - lectures, seminaries (since 2014)
  • Molecular Biology – practice (since 2012)
  • General Biochemistry - practice (since 2013)
  • Biophysics - practice (2012-2013)

Hobbies / Personal Interests

  • natural sciences
  • nature photography in general - wild-life and macrophotography, landscape photography, astrophotography etc.
  • nature protection, conservation & preservation
  • growing carnivorous plants, collecting minerals and fossils
PTX_9129-31_PS_v2.jpg PTX_2617_PS.jpg Rho_PIv1_2-PS_PI-(ExpTr)-PS3-FaF.jpg

Evening sunlight struggles through ash cloud from wildfires, Cathedral peak, Yosemite, USA, 2017

Creek in Oregon's wilderness, USA, 2017  

 

Dark skies in Death Valley, USA, 2017

 

PTX_0509_PS_-_PFS2.jpg PTX_1371_PS_v2.jpg
European mantis (Mantis religiosa). Czech Republic, 2016 Adult male jumping spider Evarcha arctuata. Czech Republic, 2014 Last sunrays of the day touching Marmolada, the highest peak of Dolomites, Italy, 2016
PTX_8871_PS_-_PFS.JPG PTX_4010_Drosera_graomogolensis_v0.jpg

Critically endangered European ground squirrel alias European souslik (Spermophilus citellus). Czech Republic, 2015

Mediterranean male dragonfly Violet Dropwing (Trithermis annulata) displays interesting mauve colouration. Turkey, 2014

South-american sundew Drosera graomogolensis, 2011

SevAm_PIv2_2-crop-PS-FaF.jpg
Bright emmision nebulae in the constellation Cygnus (Swan) - North America (NGC7000) and Pelican (IC5067) nebulae, 2016

Powerful positive cloud-to-ground lightning strike behind Bezděz castle, Czech Republic, 2014

Toronto during a nightfall from Toronto Islands, Ontario, Canada, 2013

Publications

  • author and co-author of 23 publications in international peer-reviewed journals; cumulative IF > 96
  • 256 / 268 citations (WoS/Scopus; excluding self-citations; May 2019)
  • 280 / 292 citation (WoS / Scopus; including self-citations; May 2019)
  • average citations per item: 13.33 / 13.27 (WoS / Scopus)
  • H-index: 9 / 10 (WoS/Scopus)
  • green marked publications - "(aza)phthalocyanine-based" articels :-)

 

2019

  • Lochman L, Machacek M, Miletin M, Uhlířová Š, Lang K, Kirakci  K, Zimcik P, Novakova V.
    Red-emitting fluorescence sensors for metal cations: the role of counter anions and sensing of SCN- in biological materials.
    ACS Sensors. 2019; DOI: 10.1021/acssensors.9b00081
    [Impact factor 2017: 5.711 - Q1
    ; Article Influence Score: 1.316 - Q1]

  • Reimerová P, Stariat J, Bavlovič-Piskáčková H, Jansová H, Roh J, Kalinowski D, Macháček M, Šimůnek T, Richardson D, Sterbova-Kovarikova P.
    Novel SPME fibers based on a plastic support for determination of plasma protein binding of thiosemicarbazone metal chelators: A case example of DpC, an anti-cancer drug that entered clinical trials.
    Analytical and Bioanalytical Chemistry. 2019; 411(11); 2383-2394; DOI: 10.1007/s00216-019-01681-w
    [Impact factor 2017: 3.307 - Q1
    ; Article Influence Score: 0.748 - Q1]

  • Vicen M, Vitverova B, Havelek R, Blazickova K, Machacek M, Rathouska J, Najmanová I, Dolezelova E, Prasnicka A, Sternak M, Bernabeu C, Nachtigal P.
    Regulation and role of endoglin in cholesterol-induced endothelial/vascular dysfunction in vivo and in vitro.
    FASEB Journal. 2019; 33(5); 6099-6114; DOI: 10.1096/fj.201802245R
    [Impact factor 2017: 5.595 - Q1
    ; Article Influence Score: 1.732 - Q1]

  • Kopecna M, Kováčik A, Kucera O, Machacek M, Sochorova M, Audrlicka P, VavrovaK.
    Fluorescent Penetration Enhancers Reveal Complex Interactions among the Enhancer, Drug, Solvent, and Skin.
    Molecular Pharmaceutics. 2019; 16(2); 886-897; DOI: 10.1021/acs.molpharmaceut.8b01196
    [Impact factor 2017: 4.556 - Q1
    ; Article Influence Score: 1.027 - Q1]

  • Kollar J, Macháček M, Jancarova A, Kubat P, Kucera R, Miletín M, Novakova V, ZimcikP.
    Effect of bovine serum albumin on the photodynamic activity of sulfonated tetrapyrazinoporphyrazine.
    Dyes and Pigments. 2019; 162; 358-366; DOI: 10.1016/j.dyepig.2018.10.051
    [Impact factor 2017: 3.767 - Q1
    ; Article Influence Score: 0.534 - Q1]

2018

  • Macháček M, Carter K, Kostelanský F, Miranda D, Seffouh A, Ortega J, Šimůnek T, Zimčík P, Lovell J.
    Binding of an amphiphilic phthalocyanine to pre-formed liposomes confers light-triggered cargo release.
    Journal of Materials Chemistry B. 2018; 6: 7298-7305; DOI: 10.1039/C8TB01602J
    [Impact factor 2017: 4.776 - Q1; Article Influence Score: 0.961 - Q2]

2017

  • Kopečná M, Macháček M, Prchalová E, Štěpánek P, Drašar P, Kotora M, Vávrová K.
    Galactosyl Pentadecene Reversibly Enhances Transdermal and Topical Drug Delivery.
    Pharmaceutical Research. 2017; 34(10): 2097–2108; DOI: 10.1007/s11095-017-2214-3
    [Impact factor 2017: 3.335 - Q2; Article Influence Score: 0.766 - Q2]

  • Ghazal B, Machacek M, Shalaby M, Novakova V, Zimcik P, Makhseed.
    Phthalocyanines and tetrapyrazinoporphyrazines with two cationic donuts: high photodynamic activity as a result of rigid spatial arrangement of peripheral substituents.
    Journal of Medicinal Chemistry. 2017; DOI: 10.1021/acs.jmedchem.7b00272

    [Impact factor 2017: 6.253 - Q1; Article Influence Score: 1.495 - Q1]

  • Němeček J, Sychra P, Macháček M, Benková M, Karabanovich G, Konečná K, Kavková V, Stolaříkov J, Hrabálek A, Vávrová K, Soukup O, Roh J, Klimešová V.
    Structure-activity relationship studies on 3,5-dinitrophenyl tetrazoles as antitubercular agents.

    European Journal of Medicinal Chemistry. 2017; 130(21): 419–432; DOI: 10.1016/j.ejmech.2017.02.058
    [Impact factor 2017: 4.816 - Q1
    ; Article Influence Score: 0.745 - Q2]

  • Kopečná M, Macháček M, Prchalová E, Štěpánek P, Drašar P, Kotora M, Vávrová K.
    Dodecyl amino glucoside enhances transdermal and topical drug delivery via reversible interaction with skin barrier lipids.
    Pharmaceutical Research. 2017; 34(3): 640-653; DOI: 10.1007/s11095-016-2093-z
    [Impact factor 2017: 3.335 - Q2
    ; Article Influence Score: 0.766 - Q2]

2016

  • Machacek M, Demuth J, Cermak P, Vavreckova M, Hruba L, Jedlickova A, Kubat P, Simunek T, Novakova V, Zimcik P.
    Tetra(3,4-pyrido)porphyrazines caught in the cationic cage: toward nanomolar active photosensitizers.

    Journal of Medicinal Chemistry. 2016; 59(20): 9443–9456; DOI: 10.1021/acs.jmedchem.6b01140
    [Impact factor 2017: 6.253 - Q1; Article Influence Score: 1.495 - Q1]

  • Haškova P, Jansova H, Bureš J, Macháček M, Jirkovská A, Franz KJ, Kovaříkova P, Šimůnek T.
    Cardioprotective effects of iron chelator HAPI and ROS-activated boronate prochelator BHAPI against catecholamine-induced oxidative cellular injury.
    Toxicology. 2016; 371: 17-28; DOI: 10.1016/j.tox.2016.10.004
    [Impact Factor 2017: 3.265 - Q2
    ; Article Influence Score: 0.815 - Q1]

  • Jansová H, Bureš J, Macháček M, Hašková P, Jirkovská A, Roh J, Wang Q, Franz KJ, Kovaříková P, Šimůnek T.
    Characterization of cytoprotective and toxic properties of iron chelator SIH, prochelator BSIH and their degradation products.

    Toxicology. 2015; 250: 15-24; DOI: 10.1016/j.tox.2016.03.004
    [Impact Factor 2017: 3.265 - Q2; Article Influence Score: 0.815 - Q1]

  • Machacek M, Kollár J, Miletin M, Kučera R, Kubát P, Simunek T, Novakova V, Zimcik P.
    Anionic hexadeca-carboxylate tetrapyrazinoporphyrazine: synthesis and in vitro photodynamic studies of water-soluble non-aggregating photosensitizer.
    RSC Advances. 2016; 6:10064-10077; DOI: 10.1039/C5RA25881B

    [Impact factor 2017: 2.936 - Q2
    ; Article Influence Score: 0.564 - Q2]

2015

  • Potůčková E, Roh J, Macháček M, Stariat J, Šesták V, Jansová H, Hašková P, Jirkovská A, Vávrová A, Kovaříková P, Richardson DR, Šimůnek T.
    In vitro characterization of pharmacological properties of the anticancer iron chelator Bp4eT and its phase I metabolites.
    PLoS One. 2015; 10(10): e0139929. DOI:10.1371/journal.pone.0139929
    [Impact factor 2017: 2.766 - Q1; Article Influence Score: 1.000 - Q1]

  • Jirkovská-Vávrová A, Roh J, Lenčová-Popelová O, Jirkovský E, Hrušková K, Potůčková-Macková E, Jansová H, Hašková P, Martinková P, Eisner T, Kratochvíl M, Šůs J, Macháček M, Vostatková-Tichotová L, Geršl V, Muller MT, Richardson DR, Vávrová K, Štěrba M, Šimůnek T.
    Synthesis and analysis of novel analogues of dexrazoxane and its open-ring hydrolysis product for protection against anthracycline cardiotoxicity in vitro and in vivo.
    Toxicology Research. 2015; 4: 1098-1114; DOI: 10.1039/C5TX00048C

    [Impact factor 2017: 1.890 - Q3; Article Influence Score: 0.439 - Q3]

  • Vachova L, Machacek M, Kucera R, Demuth J, Cermak P, Kopecky K, Miletin M,  Jedlickova A, Simunek T, Novakova V and Zimcik P.
    Heteroatom-substituted tetra(3,4-pyrido)-porphyrazines: a stride toward near-infrared-absorbing macrocycles.
    Organic & Biomolecular Chemistry. 2015; DOI: 10.1039/c5ob00651a
    [Impact factor 2017: 3.423 - Q2
    ; Article Influence Score: 0.725 - Q2]

  • Machacek M, Cidlina A, Novakova V, Svec J, Rudolf E,Miletin M, Kučera R, Simunek T, Zimcik P.
    Far-red absorbing cationic phthalocyanine photosensitizers: Synthesis and evaluation of the photodynamic anti-cancer activity and the mode of cell death induction.
    Journal of Medicinal Chemistry. 2015; 58(4): 1736–1749; DOI: 10.1021/jm5014852

    [Impact factor 2017: 6.253 - Q1; Article Influence Score: 1.495 - Q1]

2014

  • Potůčková E, Hrušková K, Bureš J, Kovaříková P, Špirková IA, Pravdíková K, Kolbabová L, Hergeselová T, Hašková P, Jansová H, Macháček M, Jirkovská A, Richardson V, Lane DJR, Kalinowski DS, Richardson DR, Vávrová K, Šimůnek T. 2014.
    Structure-activity relationships of novel salicylaldehyde isonicotinoyl hydrazone (SIH) analogs: iron chelation, anti-oxidant and cytotoxic properties.
    PLoS One. 2014; 9(11): e112059; DOI: 10.1371/journal.pone.0112059

    [Impact factor 2017: 2.766 - Q1; Article Influence Score: 1.000 - Q1]

  • Jansová H, Macháček M, Wang Q, Hašková P, Vávrová A, Potůčková E, Kielar F, Franz KJ, Šimůnek T. 2014.
    Comparison of various iron chelators and prochelators as protecting agents against cardiomyocyte oxidative injury.
    Free Radical Biology & Medicine. 2014. DOI: 10.1016/j.freeradbiomed.2014.06.019.

    [Impact factor 2017: 6.020 - Q1; Article Influence Score: 1.538 - Q1]

  • Potuckova E, Jansova H, Machacek M, Vavrova A, Haskova P, Tichotova L, Richardson V, Kalinowski DS, Richardson DR, Simunek T..
    Quantitative analysis of the anti-proliferative activity of combinations of selected iron-chelating agents and clinically used anti-neoplastic drugs. 
    PLoS One. 2014. 9(2). e88754. DOI: 10.1371/journal.pone.0088754.

    [Impact factor 2017: 2.766 - Q1; Article Influence Score: 1.000 - Q1]

2013

  • Makhseed S, Machacek M, Alfadly W, Tuhl A, Vinodh V, Simunek T, Novakova V, Kubat P, Rudolf E a Zimcik P.
    Water-soluble non-aggregating zinc phthalocyanine and in vitro studies for photodynamic therapy.
    Chemical Communications. 2013. 49(95): 11149-51; DOI: 10.1039/C3CC44609C

    [Impact factor 2017: 6,290 - Q1
    ; Article Influence Score: 1.436 - Q1]

  • Vávrová A, Jansová H, Macková E, Macháček M, Hašková P, Tichotová L, Štěrba M, Šimůnek T.
    Catalytic inhibitors of topoisomerase II differentially modulate the toxicity of anthracyclines towards cardiac and cancer cells.
    PLoS ONE. 2013. 8(10): 1-13; DOI: 10.1371/journal.pone.0076676

    [Impact factor 2017: 2.766 - Q1; Article Influence Score: 1.000 - Q1]

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