PharmDr. Veronika Pilařová, Ph.D.


Born: 1989, Chrudim, Czech Republic

Contact information:

Faculty of Pharmacy, Charles University
Ak. Heyrovského 1203
CZ 50005 Hradec Králové


Tel.: +420 495 067 504

ORCID ID: 0000-0002-9394-2130
Research ID: T-6365-2017
Scopus Author ID: 55211778900

Short Bio

Veronika is a post-doctoral researcher at the Department of Analytical Chemistry, Faculty of Pharmacy in Hradec Králové, Charles University.  She graduated from Pharmacy and received her Ph.D. in Pharmaceutical Analysis under the supervision of Prof. L. Nováková. During her Ph.D., she was dealing with current trends in microextraction approaches based on solid phase extraction and liquid-liquid extraction for fast UHPLC-MS and UHPSFC-MS analyses.

Currently, her research is focused on the development and optimization of new methods using supercritical and gas-expanded fluids for extraction and analysis of biologically active compounds from various matrices.

Present position:


  • 2015 – 2017: License study Interactive statistical data analysis, University of Pardubice.
  • Guarantor of the study: Prof. RNDr. Milan Meloun, DrSc.
  • 2013- 2017: Ph.D. study (Pharmaceutical Analysis) at the Department of Analytical Chemistry, Faculty of Pharmacy, Charles University
  • Doctoral thesis: Development and optimization of sample preparation step for fast chromatographic analysis.
  • 2008 - 2013: Master studies (Pharmacy) at the faculty of Pharmacy, Charles University

Research fellowships:

  • 2018: 1 week in Lund University, Department of Chemistry, Center for Analysis and Synthesis (CAS), Green Technology Group, supervisor: prof. Charlotta Turner. 8th – 14th October,
  • 2018: 3 months in Lund University, Department of Chemistry, Center for Analysis and Synthesis (CAS), Green Technology Group, supervisor: prof. Charlotta Turner. 1st March – 31st May.
  • 2016: 2 weeks in Palacky University Olomouc, Faculty of Science, Biological Center in Holice – traineeship at Laboratory of Growth Regulators, Olomouc, CR 8th – 21st August. 
  • 2015: 6 weeks in University of Oslo, School of Pharmacy, Department of Pharmaceutical Chemistry, Stig Pederson-Bjergaard’s research group, 11th April – 22nd May
  • 2014: 8 weeks in Umeå University, Umeå Plant Science Centre, Karin Ljung Science Group, Umeå, Sweden, 5th May – 11th June; 15th – 30th November
  • 2014: Quinta Analytica s.r.o. – traineeship at Department of Biopharmaceutical Analysis, Prague, CR, 24th – 28th March
  • 2014: Palacky University Olomouc, Faculty of Science, Biological Center in Holice – traineeship at Laboratory of Growth Regulators (dr. O. Novák), Olomouc, CR 14th – 18th April

Research interests:

  • Supercritical fluid chromatography (SFC)
  • Supercritical fluid extraction (SFE)
  • Gas expanded liquid extraction
  • Ultra high-performance liquid chromatography (UHPLC)
  • Mass spectrometry (MS)
  • Hyphenated techniques (UHPLC-MS, SFC-MS)
  • Sample preparation including traditional (solid phase extraction, liquid-liquid extraction) and modern approaches (micro-SPE in pipette tips, MEPS, PALME)

Teaching activity:

  • Analytical chemistry
  • Instrumental methods
  • Special methods of instrumental analysis
  • Selected separation methods
  • Mass spectrometry

Professional Membership:

  • Czech Society for Mass spectrometry
  • Spectroscopic Society of J. M. M.

Grant projects:

  • Principal investigator - Grant agency of Charles University project GA UK No.1948214; SFC-MS technique for analysis of biological material with emphasis on matrix effects. (2015)
  • Principal investigator - Norway Grants and EEA grant project No. NF-CZ07-INS-4-046-2014; New important microextraction techniques for rapid sampling and sample preparation. (2014)

Co-worker on:

  • Grant agency of the Czech republic project No. 301/17/05409S
  • UNCE project No. 204026/2012
  • Grant agency of Charles University project GA UK No. 253115
  • AZV ČR grant project No. 15-29225A
  • Grant agency of the Czech republic project No. 15-10781S
  • TEAB project No. CZ.1.07/2.3.00/20.0235 (2014)



  1. Pilařová Veronika, Al Hamimi Said, Cunico P. Larissa, Nováková Lucie, Turner Charlotta.
    Extending the design space in solvent extraction – from supercritical fluid to pressurized liquids using carbon dioxide, ethanol, ethyl lactate, and water in a wide range of proportions.
    Green Chemistry, 2019, vol. 21, p. 5427-5436 (IF: 9.405, Q1, AIS: 1.792)
  2. Pilařová Veronika, Plachká Kateřina, Khalikova Maria A., Svec Frantisek, Nováková Lucie.
    Recent developments in supercritical fluid chromatography – mass spectrometry: Is it a viable option for analysis of complex samples?
    TrAC Trends in Analytical Chemistry, 2019, vol. 112, p. 212-225 (IF: 7.034, Q1, AIS: 1.754)
  3. Skjærvø Øystein, Pilařová Veronika, Khalikova Maria, Reubsaet Leon, Halvorsen Trine Grønhaug, Gjelstad Astrid, Øiestad Elisabeth Leer, Wilson Steven, Pedersen-Bjergaard Stig.
    Those Who Can, Teach: Pharma Stars.
    The Analytical Scientist, 2018, vol. 70, p. 40 – 45. (no IF)
  4. Pourová Jana, Najmanová Iveta, Vopršalová Marie, Migkos Thomas Pilařová Veronika, Applová Lenka, Nováková Lucie, Mladěnka Přemysl.
    Two flavonoid metabolites, 3,4 dihydroxyphenylacetic acid and 4 methylcatechol, relax arteries ex vivo and decrease blood pressure in vivo.
    Vascular Pharmacology, 2018, vol. 11, p. 36 – 43. (IF: 3.607; Q1)
  5. Pěnčík Aleš, Casabova-Sáez Rubén, Pilařová Veronika, Žukauskaité Asta, Pinto Rui, Micol Luis José, Ljung Karin, Novák Ondřej.
    Ultra-rapid auxin metabolite profiling for high-throughput mutant screening in Arabidopsis.
    Journal of Experimental Botany, 2018, vol. 69, p. 2569 – 2579. (IF: 5.830)
  6. Piera Porcu Elena, Cossu Massimo, Rassu Giovanna, Giunchedi Paolo, Cerri Guido, Pourová Jana, Najmanová Iveta, Migkos Thomas, Pilařová Veronika, Nováková Lucie, Mladěnka Přemysl, Gavini Elisabetta
    Aqueous injection of quercetin: An approach for confirmation of its direct in vivo cardiovascular effects
    International Journal of Pharmaceutics, 2018, vol. 541, p. 224 – 233 (IF:3.649)
  7. Pilařová Veronika, Plachká Kateřina, Chrenková Lucia, Najmanová Iveta, Mladěnka Přemysl, Švec František, Novák Ondřej, Nováková Lucie.
    Simultaneous determination of quercetin and its metabolites in rat plasma by using ultra-high performance liquid chromatography tandem mass spectrometry.
    Talanta, 2018, vol. 185, p. 71 – 79. (IF: 4.162)
  8. Kočová Vlčková Hana, Pilařová Veronika, Svobodová Pavlína, Plíšek Jiří, Švec František, Nováková Lucie.
    Current state of bioanalytical chromatography in clinical analysis.
    Analyst, 2018, vol. 143, p. 1305 – 1325. (IF: 3.885)
  9. Vlčková Hana, Pilařová Veronika, Novák Ondřej, Solich Petr, Nováková Lucie
    Micro-SPE in pipette tips as a tool for analysis of small-molecule drugs in serum.
    Bioanalysis, 2017, vol. 9, p. 887 – 901 (IF: 2.673)
  10. Pilařová Veronika, Sultani Mumtaz, Ask Kristine Skoglund, Nováková Lucie, Pedersen-Bjergaard Stig, Gjelstad Astrid
    One-step extraction of polar drugs from plasma by Parallel Artificial Liquid Membrane Extraction.
    Journal of Chromatography B, 2017, vol. 1043, p. 25 - 32 (IF: 2.487)
  11. Pilařová Veronika, Gottvald Tomáš, Svoboda Pavel, Novák Ondřej, Benešová Karolína, Benešová  Karolína, Nováková Lucie
    Development and optimization of ultra-high performance supercritical fluid chromatography mass spectrometry method for high-throughput determination of tocopherols and tocotrienols in human serum.
    Anal. Chim. Acta, 2016, vol. 934, p. 252-265. (IF: 4.712)
  12. Filipský Tomáš, Říha Michal, Hašková Pavlína, Pilařová Veronika, Nováková Lucie, Semecký Vladimír, Vávrová Jaroslava, Holečková Magdaléna, Palicka Vladimir,  Šimůnek Tomáš, Hrdina Radomír, Mladěnka Přemysl
    Intravenous rutin in rat exacerbates isoprenaline-induced cardiotoxicity likely due to intracellular oxidative stress.
    Redox Report 2016, vol. 21, p. 78 - 90. (IF:2.606)
  13. Najmanová  Iveta, Pourová Jana, Vopršalová  Marie, Pilařová Veronika, Semecký  Vladimír, Nováková Lucie, Mladěnka  Přemysl
    Flavonoid metabolite 3-(3-hydroxyphenyl)propionic acid formed by human microflora decreases arterial blood pressure in rats.
    Molecular Nutrition and Food Research, 2016, vol. 60, p. 981-991.  (IF: 4.551)
  14. Hirsová Petra, Doležel Jan, Kučerová-Chlupáčová  Marta, Kuneš  Jiří, Pilařová  Veronika, Nováková Lucie, Opletalová Veronika
    Molbank, 2015, vol. M872, p. 1-7.  (IF: 0.000)
  15. Říha  Michal, Vopršalová  Marie, Pilařová Veronika, Semecký Vladimír, Holečková  Magdalena, Vávrová  Jaroslava, Palička  Vladimír,  Filipský  Tomáš,  Hrdina  Radomír, Nováková Lucie, Mladěnka  Přemysl
    Oral administration of quercetin is unable to protect against isoproterenol cardiotoxicity.
    Naunyn-Schmiedeberg´s Arch Pharmacol, 2014, vol. 387, p. 823-835. (IF: 2.471)

Other activities:

  • 2014 – 2019: Author of the project for presentation of scientific and research activities in of Faculty of Pharmacy on many events (HPLC 2017 in Prague, Festival vědy, Vědecký jarmark Nábřeží vysokých škol, …)
  • 2019: The member of Organizing committee for School of Mass Spectrometry, Špindlerův Mlýn, CZ, 9th – 13th September and Czech Chromatographic School HPLC 2019, Zaječí, CZ, 12th – 15th May
  • 2018: The member of Organizing committee for Mass Spectrometry School, Špindlerův Mlýn, CZ, 9th – 14th September
  • 2017: The member of Organizing committee for Czech Chromatographic School HPLC 2017, Rožnov pod Radhoštěm, CZ, 12th – 15th March
  • 2013 – 2017: Member of The Academic Senate of Faculty of Pharmacy in Hradec Králové

Hobbies/Personal interests:

  • sport – capoeira, aerial hoop, yoga
  • cultural events and their organizing
  • travelling

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