Prof. PharmDr. Kateřina Vávrová, Ph.D.

Research interests

• skin barrier lipids (lipid synthesis, analysis and biophysics to understand their role in the skin permeability barrier)
• transdermal and topical drug delivery, including novel permeation enhancers and lipid-based carriers

Academic qualifications, professional experience

• since 2017: professor in Medicinal chemistry (Charles University, Faculty of Pharmacy)
• since 2009: associate professor in Medicinal chemistry (Charles University, Faculty of Pharmacy)
• 2005 - 2011: researcher at the Centre “New antivirals and antineoplastics“ and “Research of new drugs“
• 2004 - 2005: visiting researcher at the University of Franche-Comte, Faculty of Medicine and Pharmacy, Laboratory of Cutaneous Biology, Besancon, France
• 2003 - 2009: researcher at the Department of Inorganic and Organic Chemistry, Charles University, Faculty of Pharmacy
• 1999 - 2003: PhD in Bioorganic chemistry, Charles University
• 1994 - 1999: MSc in Pharmacy, Charles University

Teaching and popularization of science

• 6 Ph.D. students supervised at present, 8 Ph.D. graduates, 31 students of master studies, 5 PharmD students (experimental work), two high school student research projects
• teaching of Organic chemistry I, Organic chemistry II, and Solved problems in organic chemistry
• e-learning course “Solved problems in organic chemistry” (in Czech)
• popularization of science, for example presentations at Schola ludus and Summer School (2013), Day of Science (2012), Czech TV series “About Science and Scientists” (2014)

Selected scientific grants (PI)

• 2024-2027: AZV project NW24-08-00289 (with Motol hospital) Skin Barrier Lipidomics as a Noninvasive Method for Predicting Late Adverse Events in Childhood Cancer Survivors
• 2022-2024: ERA-NET Cofund EuroNanoMed3/TAČR Pre-clinical development of an acylceramide nanostructured delivery system to rescue the skin barrier in patients with ichthyosis (LIPARCI)
• 2022-2024: GAČR/DFG, LA project 22-20839K (with Huster Lab, University of Leipzig): Novel Insights into the Nanostructure of the Skin Lipid Barrier by Biophysical Methods
• 2019-2021: GAČR-DFG project 19-09135J (with Huster Lab, University of Leipzig): Ultralong chain ceramides in membrane models of healthy and diseased skin barrier
• 2019-2021: GAČR 19-09600S (co-PI, with University of Chemistry and Technology Prague) Integrated design methodology of nanoformulation processes for (trans )dermal delivery of actives
• 2016-2018: GAČR-DFG 16-25687J (with Freie Universitaet Berlin): Interdependencies between Inflammatory Processes and Barrier Lipids in Diseased Skin

Membership

Editorial Board Member for Scientific Reports, a Nature Research journal (2017-2024), European Epidermal Barrier Research Network, EpilipidNet, Scientific council of the Faculty of Pharmacy, Charles University (since 2014), Scientific council of the Faculty of Military Health, University of Defense (2015-16), Ph.D. board in Bioorganic Chemistry, Ph.D. board in Pharmaceutical Technology, PharmD, MSc and Bc boards at the Faculty of Pharmacy, Charles University in Prague, Czech Health Research Council (AZV ČR) - panel 08 Biomedical technologies (2015-2017), Scientific jury of the Prix Sanofi de pharmacie (2012-2014), American Society for Biochemistry and MOlecular Biology, Czech Chemical Society

Invited lectures

last 5 years: Skin Forum London (2024), Prague-Weizmann Institute Summer School (2024), Biobarriers worshop Malmö (2024) 7^th Barrande Bioscience Meeting (2022), KSID Annual meeting (2022), “Skin Barrier and Stratum Corneum Lipids” online workshop (2021), Gordon Research Conference “Barrier function of mammalian skin” Waterville Valley, NH, USA (2019), 

Awards

Publications

128 publications with IF, over 3200 citations, H-index 36 (WoS), 2 Czech patents, 4 book chapters, ORCID, ResearchGate, Google Scholar

2022

Opalka L, Meyer JM, Ondrejcekova V, Svatosova L, Radner FPW, Vavrova K. omega-O-Acylceramides but not omega-hydroxy ceramides are required for healthy lamellar phase architecture of skin barrier lipids. J Lipid Res. 2022; 63(6):100226.

Sagrafena I, Paraskevopoulos G, Pullmannova P, Opalka L, Nováčková A, Lourantou O, Vavrova K. Assembly of Human Stratum Corneum Lipids In Vitro: Fluidity Matters. J. Invest. Dermatol. 2022; 142(7):2036-2039.e3 

Sochorová M, Vávrová K, Fedorova M, Ni Z, Slenter D, Kutmon M, Willighagen EL, Letsiou S, Töröcsik D, Marchetti-Deschmann M, Zoratto S, Kremslehner C, Gruber F. Research Techniques Made Simple: Lipidomic Analysis in Skin Research. J. Invest. Dermatol. 2022; 142(1), 4-11.e1

Fandrei F, Engberg O, Opálka L, Jančálková P, Pullmannová P, Steinhart M, Kováčik A, Vávrová K, Huster D. Cholesterol sulfate fluidizes the sterol fraction of the stratum corneum lipid phase and increases its permeability. J. Lipid Res. 2022; 63(3):100177.

Kopečná M, Macháček M, Roh J, Vávrová K. Proline, hydroxyproline, and pyrrolidone carboxylic acid derivatives as highly efficient but reversible transdermal permeation enhancers. Sci Rep. 2022; 12(1).

Haskova P, Applova L, Jansova H, Homola P, Franz KJ, Vavrova K, Roh J, Simunek T. Examination of diverse iron-chelating agents for the protection of differentiated PC12 cells against oxidative injury induced by 6-hydroxydopamine and dopamine. Sci Rep. 2022 ;12(1):9765.

Hergesell K, Valentová K, Velebný V, Vávrová K, Dolečková I. Common cosmetic compounds can reduce air pollution-induced oxidative stress and pro-inflammatory response in the skin. Skin Pharmacol. Physiol. 2022; DOI: 10.1159/000522276.

Panoutsopoulou E, Zbytovská J, Vávrová K, Paraskevopoulos G. Phospholipid-Based Microemulsions for Cutaneous Imiquimod Delivery. Pharmaceuticals 2022;15 (5), 515

2021

Novackova, A.;  Sagrafena, I.;  Pullmannova, P.;  Paraskevopoulos, G.;  Dwivedi, A.;  Mazumder, A.;  Růžičková, K.;  Slepicka, P.;  Zbytovská, J.; Vávrová, K., Acidic pH is Required for the Multilamellar Assembly of Skin Barrier Lipids in Vitro. J. Invest. Dermatol. 2021, 141(8), 1915-1921.e4.

Kováčik, A.; Pullmannová, P.; Opálka, L.; Šilarová, M.; Maixner, J.; Vávrová, K. Effects of (R)- and (S)-α-Hydroxylation of Acyl Chains in Sphingosine, Dihydrosphingosine and Phytosphingosine Ceramides on Phase Behavior and Permeability of Skin Lipid Models. Int. J. Mol. Sci. 2021, 22, 7468.

Kopečná, M.;  Kováčik, A.;  Novák, P.;  Boncheva Bettex, M.; Vávrová, K., Transdermal Permeation and Skin Retention of Diclofenac and Etofenamate/Flufenamic Acid From Over-the-Counter Pain Relief Products. J. Pharm. Sci. 2021.

2020

Engberg O,^# Kováčik A,^# Pullmannová P,^# Juhaščik M, Opálka L, Huster D, Vávrová K. The Sphingosine and Acyl Chains of Ceramide [NS] Show Very Different Structure and Dynamics Challenging Our Understanding of the Skin Barrier. Angew. Chem. Int. Ed. 2020, 132 (40), 17536-17540. ^#equal contribution

Opálka, L.;  Kováčik, A.;  Pullmannová, P.;  Maixner, J.; Vávrová, K., Effects of omega-O-acylceramide structures and concentrations in healthy and diseased skin barrier lipid membrane models. J. Lipid Res. 2020, 61 (2), 219-228.

Kováčik, A.;  Pullmannová, P.;  Pavlíková, L.;  Maixner, J.; Vávrová, K., Behavior of 1-Deoxy-, 3-Deoxy- and N-Methyl-Ceramides in Skin Barrier Lipid Models. Sci. Rep. 2020, 10 (1).

Kovacik, A.;  Kopecna, M.; Vavrova, K., Permeation enhancers in transdermal drug delivery: benefits and limitations. Expert Opin. Drug Deliv. 2020, 17 (2), 145-155. 50 free e-prints

Jevtić M, Löwa A, Nováčková A, Kováčik A, Kaessmeyer S, Erdmann G, Vávrová K, Hedtrich S. Impact of intercellular crosstalk between epidermal keratinocytes and dermal fibroblasts on skin homeostasis. Biochim Biophys Acta Mol Cell Res. 2020;1867(8):118722.

Gendrisch F, Nováčková A, Sochorová M, Haarhaus B, Vávrová K, Schempp CM, Wölfle U. Gentiana lutea Extract Modulates Ceramide Synthesis in Primary and Psoriasis-Like Keratinocytes. Molecules. 2020;25(8):1832.

Elpelt, A.;  Ivanov, D.;  Novackova, A.;  Kovacik, A.;  Sochorova, M.;  Saeidpour, S.;  Teutloff, C.;  Lohan, S. B.;  Lademann, J.;  Vavrova, K.;  Hedtrich, S.; Meinke, M. C., Investigation of TEMPO partitioning in different skin models as measured by EPR spectroscopy - Insight into the stratum corneum. J. Magn. Reson. 2020, 310, 106637.

2019

Kopečná M, Macháček M, Nováčková A, Paraskevopoulos G, Roh J, Vávrová K. Esters of terpene alcohols as highly potent, reversible, and low toxic skin penetration enhancers. Sci. Rep. 2019, 9:14617.

Karabanovich G, Dušek J, Savková K, Pavliš O, Pávková I, Korábečný J, Kučera T, Kočová Vlčková H, Huszár S, Konyariková Z, Konečná K, Jand'ourek O, Stolaříková J, Korduláková J, Vávrová K, Pávek P, Klimešová V, Hrabálek A, Mikušová K, Roh J. Development of 3,5-Dinitrophenyl-Containing 1,2,4-Triazoles and Their Trifluoromethyl Analogues as Highly Efficient Antitubercular Agents Inhibiting Decaprenylphosphoryl-β-d-ribofuranose 2'-Oxidase. J Med Chem. 2019; 62(17):8115-8139

Pullmannova P, Ermakova E, Kovacik A, Opalka L, Maixner J, Zbytovska J, Kucerka N, Vavrova K. Long And Very Long Lamellar Phases In Model Stratum Corneum Lipid Membranes. J. Lipid Res. 2019; 60(5):963-971.

Hausmann Ch, Zoschke Ch, Wolff Ch, Darvin M, Sochorová M, Kováčik A, Wanjiku B, Schumacher F, Tigges J, Kleuser B, Lademann J, Fritsche E, Vávrová K, Ma N, Schaefer-Korting M. Fibroblast origin shapes tissue homeostasis, epidermal differentiation, and drug uptake. Sci. Rep. 2019, 9:2913.

Čuříková-Kindlová BA, Diat O, Štěpánek F, Vávrová K, Zbytovská J. Probing the interactions among sphingosine and phytosphingosine ceramides with non- and alpha-hydroxylated acyl chains in skin lipid model membranes. Int J Pharm. 2019;563:384-394.

Kopečná M, Kováčik A, Kučera O, Macháček M, Sochorová M, Audrlická P, Vávrová K. Fluorescent Penetration Enhancers Reveal Complex Interactions among the Enhancer, Drug, Solvent, and Skin. Mol. Pharm. 2019, 16(2):886-897.

Sochorová M, Audrlická P, Červená M, Kováčik A, Kopečná M, Opálka L, Pullmannová P, Vávrová K. Permeability and microstructure of cholesterol-depleted skin lipid membranes and human stratum corneum. J. Colloid Interface Sci. 2019, 535:227-238

2018

Mauldin EA, Crumrine D, Casal LM, Jeong S, Opálka L, Vavrova K, Uchida Y, Park K, Craiglow B, Choate KA, Shin‖ K, Lee‖ Y, Grove GL, Wakefield JS, Khnykin D, Elias PM. Cellular and Metabolic Basis for the Ichthyotic Phenotype in NIPAL4 (Ichthyin) Deficient Canines. Am. J. Pathol. 2018;188(6):1419-1429. DOI: 10.1016/j.ajpath.2018.02.008

Balansin Rigon R, Kaessmeyer S, Wolff C, Hausmann C, Zhang N, Sochorová M, Kováčik A, Haag R, Vávrová K, Ulrich M, Schäfer-Korting M, Zoschke, C. Ultrastructural and molecular analysis of ribose-induced glycated reconstructed human skin. Int J Mol Sci. 2018;19, 3521.

Kováčik A, Vogel A, Adler J, Pullmannová P, Vávrová K, Huster D. Probing the role of ceramide hydroxylation in skin barrier lipid models by 2H solid-state NMR spectroscopy and X-ray powder diffraction. Biochim Biophys Acta. 2018;1860(5):1162-1170

Kováčik A, Pullmannová P, Maixner J, Vávrová K. Effects of Ceramide and Dihydroceramide Stereochemistry at C-3 on the Phase Behavior and Permeability of Skin Lipid Membranes. Langmuir. 2018, 34 (1), 521–529

Hájek R, Lísa M, Khalikova M, Jirásko R, Cífková E, Študent V Jr, Vrána D, Opálka L, Vávrová K, Matzenauer M, Melichar B, Holčapek M. HILIC/ESI-MS determination of gangliosides and other polar lipid classes in renal cell carcinoma and surrounding normal tissues. Anal Bioanal Chem. 2018;410(25):6585-6594.

Hrušková K, Potůčková E, Opálka L, Hergeselová T, Hašková P, Kovaříková P, Šimůnek T, Vávrová K. Structure-Activity Relationships of Nitro-Substituted Aroylhydrazone Iron Chelators with Antioxidant and Antiproliferative Activities. Chem Res Toxicol. 2018;31(6):435-446. doi: 10.1021/acs.chemrestox.7b00324.

2017

Čuříková BA, Procházková K, Filková B, Diblíková P, Svoboda J, Kováčik A, Vávrová K, Zbytovská J. Simplified stratum corneum model membranes for studying the effects of permeation enhancers. Int. J. Pharm. 2017, 534(1-2):287-296.

Svoboda M, Hlobilová M, Marešová M, Sochorová M, Kováčik A, Vávrová K, Dolečková I. Comparison of suction blistering and tape stripping for analysis of epidermal genes, proteins and lipids. Arch. Dermatol. Res. 2017, 309(9):757-765.

Sochorová M, Staňková K, Pullmannová P, Kováčik A, Zbytovská J, Vávrová K. Permeability Barrier and Microstructure of Skin Lipid Membrane Models of Impaired Glucosylceramide Processing. Sci. Rep. 2017, 7:6470.

Kopečná M, Macháček M, Prchalová E, Štěpánek P, Drašar P, Kotora M, Vávrová K. Galactosyl Pentadecene Reversibly Enhances Transdermal and Topical Drug Delivery. Pharm. Res. 2017; 34(10), 2097-2108.

Pullmannová P, Pavlíková L, Kováčik A, Sochorová M, Školová B, Slepička P, Maixner J, Zbytovská J, Vávrová K. Permeability and microstructure of model stratum corneum lipid membranes containing ceramides with long (C16) and very long (C24) acyl chains. Biophys. Chem. 2017, 224:20-31

Wallmeyer L, Dietert K, Sochorová M, Gruber AD, Kleuser B, Vávrová K, Hedtrich S. TSLP is a direct trigger for T cell migration in filaggrin-deficient skin equivalents. Sci. Rep. 2017, 7:774.

Kováčik A, Šilarová M, Pullmannová P, Maixner J, Vávrová K. Effects of 6-hydroxyceramides on the thermotropic phase behavior and permeability of model skin lipid membranes. Langmuir 2017, 33(11):2890-2899

Schroeter A, Stahlberg S, Školová B, Sonnenberger S, Eichner A, Huster D, Vavrova K, Hauß T, Dobner B, Neubert R, Vogel A, Phase separation in Ceramide [NP] containing lipid model membranes: Neutron diffraction and solid-state NMR. Soft Matter, 2017, 13, 2107-2119.

Kopečná M, Macháček M, Prchalová E, Štěpánek P, Drašar P, Kotora M, Vávrová K. Dodecyl amino glucoside enhances transdermal and topical drug delivery via reversible interaction with skin barrier lipids. Pharm. Res. 2017; 34:640-653. Springer sharing link. 

Školová B, Kováčik A, Tesař O, Opálka L, Vávrová K. Phytosphingosine, sphingosine and dihydrosphingosine ceramides in model skin lipid membranes: permeability and biophysics. Biochim. Biophys. Acta - Biomembranes. 2017; 1859(5):824-834.

Vávrová K, Kováčik A, Opálka L. Ceramides in the skin barrier. Eur. Pharm. J. 2017, 64(2): 28–35.

Bezrouk A, Fiala Z, Kotingová L, Selke Krulichová I, Kopečná M, Vávrová K. SAMPA: A free software tool for skin and membrane permeation data analysis. Toxicol. in Vitro 2017, 44:361-371.

Roh J, Karabanovich G, Vlčková H, Carazo A, Němeček J, Sychra P, Valášková L, Pavliš O, Stolaříková J, Klimešová V, Vávrová K, Pávek P, Hrabálek A. Development of Water-Soluble 3,5-Dinitrophenyl Tetrazole and Oxadiazole Antitubercular Agents. Bioorg. Med. Chem. 2017,25(20):5468-5476.

Němeček J, Sychra P, Macháček M, Benková M, Karabanovich G, Konečná K, Kavková V, Stolaříková J, Hrabálek A, Vávrová K, Soukup O, Roh J, Klimešová V. Structure-activity relationship studies on 3,5-dinitrophenyl tetrazoles as antitubercular agents. Eur. J. Med. Chem. 2017; 130: 419-432.

Karabanovich G, Němeček J, Valášková L, Carazo A, Konečná K, Stolaříková J, Hrabálek A, Pavliš O, Pávek P, Vávrová K, Roh J, Klimešová V.  S-substituted 3,5-dinitrophenyl 1,3,4-oxadiazole-2-thiols and tetrazole-5-thiols as highly efficient antitubercular agents. Eur. J. Med. Chem. 2017; 126: 369-383

2016

Opálka L, Kováčik A, Maixner J, Vávrová K. Omega-O-acylceramides in Skin Lipid Membranes: Effects of Concentration, Sphingoid Base and Model Complexity on Microstructure and Permeability. Langmuir 2016; 32(48):12894–12904. DOI: 10.1021/acs.langmuir.6b03082 - Open Access

Zoschke C, Ulrich M, Sochorová M, Wolff C, Vávrová K, Ma N, Ulrich C, Brandner J, Schäfer-Korting M. The barrier function of organotypic non-melanoma skin cancer models. J. Control. Release. 2016; 233:10-18.

Kováčik A, Opálka L, Šilarová M, Roh J, Vávrová K. Synthesis of 6-Hydroxyceramide Using Ruthenium-Catalyzed Hydrosilylation-Protodesilylation. Unexpected Formation of a Long Periodicity Lamellar Phase in Skin Lipid Membranes. RSC Adv. 2016, 6(77), 73343-73350.

Školová B; Janůšová B; Vávrová K. Ceramides with a pentadecasphingosine chain and short acyls have strong permeabilization effects on skin and model lipid membranes. Biochim Biophys Acta. Biomembranes 2016; 1858(2):220–232.

Vávrová K. Emerging small-molecule compounds for treatment of atopic dermatitis: a review. Exp. Opin. Ther. Patents 2016; 26(1):21-34. 50 free reprints

Hrušková K, Potůčková E, Hergeselová T, Liptáková L, Hašková P, Mingas P, Kovaříková P, Šimůnek T, Vávrová K. Aroylhydrazone Iron Chelators: Tuning Antioxidant and Antiproliferative Properties by Hydrazide Modifications. Eur. J. Med. Chem. 2016; in press. doi: 10.1016/j.ejmech.2016.05.015

Karabanovich G, Zemanová J, Smutný T, Székely R, Šarkan M, Centárová I, Vocat A, Pávková I, Čonka P, Němeček J, Stolaříková J, Vejsová M, Vavrova K, Klimešová K, Hrabalek A, Pavek P, Cole ST, Mikušová K, Roh J. Development of 3,5-Dinitrobenzylsulfanyl-1,3,4-Oxadiazoles and Thiadiazoles as Selective Antitubercular Agents Active Against Replicating and Nonreplicating Mycobacterium tuberculosis. J. Med. Chem. 2016, 59 (6), pp 2362–2380.

Roh J, Karabanovich G, Novakova V, Šimůnek T, Vávrová K. Large-Scale Synthesis of Piperazine-2,6-dione and Its Use in the Synthesis of Dexrazoxane Analogues. Synthesis 2016; DOI: 10.1055/s-0035-1562618

Vávrová K, Hrabálek A. Amino Acid-Based Transdermal Penetration Enhancers. In: Percutaneous Penetration Enhancers Chemical Methods in Penetration Enhancement. Modification of the Stratum Corneum, 2016, Editors. Nina Dragicevic, Howard I. Maibach, Edition: 1, Springer-Verlag Berlin Heidelberg, pp.325-336. DOI: 10.1007/978-3-662-47039-8, ISBN 978-3-662-47038-1

Hrabálek A, Vávrová K. Transkarbams: Transdermal Penetration-Enhancing Carbamates. In: Percutaneous Penetration Enhancers Chemical Methods in Penetration Enhancement. Modification of the Stratum Corneum, 2016, Editors. Nina Dragicevic, Howard I. Maibach, Edition: 1, Springer-Verlag Berlin Heidelberg, pp.309-323. DOI: 10.1007/978-3-662-47039-8, ISBN 978-3-662-47038-1

2015

Opálka L, Kováčik A, Sochorová M, Roh J, Kuneš J, Lenčo J, Vávrová K. Scalable Synthesis of Human Ultralong Chain Ceramides. Org. Lett. 2015; 17(21):5456-5459. DOI: 10.1021/acs.orglett.5b02816 - Open Access

Wallmeyer L; Lehnen D; Eger N; Sochorová M; Opálka L; Kováčik A; Vávrová K, Hedtrich S. Stimulation of PPARalpha Normalizes the Skin Lipid Ratio and Improves the Skin Barrier of Normal and Filaggrin Deficient Reconstructed Skin. J. Dermatol. Sci. 2015; 80(2):102–110. doi:10.1016/j.jdermsci.2015.09.012.

Paloncýová M, Vávrová K, Sovová Ž, DeVane R, Otyepka M, Berka K. Structural Changes in Ceramide Bilayers Rationalize Increased Permeation through Stratum Corneum Models with Shorter Acyl Tails. J Phys Chem B. 2015;119(30):9811-9

Stahlberg S, Školová B, Madhu PK, Vogel A, Vávrová K, Huster D. Probing the Role of the Ceramide Acyl Chain Length and Sphingosine Unsaturation in Model Skin Barrier Lipid Mixtures by 2H Solid-State NMR Spectroscopy. Langmuir 2015;31(17):4906-15

Witting M, Boreham A, Brodwolf R, Vávrová K, Alexiev U, Friess W, Hedtrich S. Interactions of Hyaluronic Acid with the Skin and Implications for the Dermal Delivery of Biomacromolecules. Mol Pharm. 2015;12(5):1391-401

Karabanovich G, Roh J, Soukup O, Pasdiorová M, Tambor V, Stolaříková J, Vejsová M, Vávrová K, Klimešová V, Hrabálek A. Tetrazole Regioisomers in the Development of Nitro Group-Containing Antitubercular Agents. MedChemComm. 2015;6:174-181

Potůčková E; Roh J; Macháček M; Sahni S; Stariat J; Šesták V; Jansová H; Hašková P; Jirkovská A; Vávrová K; Kovaříková P; Kalinowski DS; Richardson DR; Simunek T. In Vitro Characterization of the Pharmacological Properties of the Anti-Cancer Chelator, Bp4eT, and its Phase I Metabolites. PLOS One. 2015; 10(10):e0139929. DOI: 10.1371/journal.pone.0139929.

Jirkovská-Vávrová A., Roh J., Lenčová-Popelová O., Jirkovský E., Hrušková K., Potůčková-Macková E., Jansová H., Hašková P., Martinková P., Eisner T., Kratochvíl M., Šůs J., Macháček M., Vostatková-Tichotová L., Geršl V., Kalinowski D.S., Muller M., Richardson D.R., Vávrová K., Štěrba M., Šimůnek T. Synthesis and analysis of novel analogues of dexrazoxane and its open-ring hydrolysis product for protection against anthracycline cardiotoxicity in vitro and in vivo. Toxicology Res. 2015;4:1098-1114

2014

Vávrová K, Henkes D, Strüver K, Sochorová M, Školová B, Witting M, Friess W, Schreml S, Meier RJ, Schäfer-Korting M, Fluhr JW, Küchler S. Filaggrin Deficiency Leads to Impaired Lipid Profile and Altered Acidification Pathways in a 3 D Skin Construct. J. Invest. Dermatol. 2014, 134(3): 746-753.

Kováčik A, Roh J, Vávrová K. The chemistry and biology of 6 hydroxyceramide, the youngest member of the human sphingolipid family. ChemBioChem 2014, 15:1555-1562.

Pullmannová P, Staňková K, Pospíšilová M, Skolová B, Zbytovská J, Vávrová K. Effects of sphingomyelin/ceramide ratio on the permeability and microstructure of model stratum corneum lipid membranes. Biochim Biophys Acta. 2014, 1838(8):2115-2126.

Školová B, Jandovská K, Pullmannová P, Tesař O, Roh J, Hrabálek A, Vávrová K. The role of the trans double bond in skin barrier sphingolipids: permeability and infrared spectroscopic study of model ceramide and dihydroceramide membranes. Langmuir. 2014; 30(19):5527-5535.

Školová B, Hudská K, Pullmannová P, Kováčik A, Palát K, Roh J, Fleddermann J, Estrela-Lopis I, Vávrová K. Different Phase Behavior and Packing of Ceramides with Long (C16) and Very Long (C24) Acyls in Model Membranes: Infrared Spectroscopy Using Deuterated Lipids. J Phys Chem B. 2014, 118 (35), 10460–10470.

Diblíková D, Kopečná M, Školová B, Krečmerová M, Roh J, Hrabálek A, Vávrová K. Transdermal Delivery and Cutaneous Targeting of Antivirals using a Penetration Enhancer and Lysolipid Prodrugs. Pharm. Res. 2014, 31(4):1071-1081.

Karabanovich G, Roh J, Smutný T, Němeček J, Vicherek P, Stolaříková J, Vejsová M, Dufková I, Vávrová K, Pávek P, Klimešová V, Hrabálek A. 1-Substituted-5-[(3,5-dinitrobenzyl)sulfanyl]-1H-tetrazoles and their isosteric analogs: A new class of selective antitubercular agents active against drug-susceptible and multidrug-resistant mycobacteria. Eur. J. Med. Chem. 2014; 82:324-340.

Potůčková E, Hrušková K, Bureš J, Kovaříková P, Špirková IA, Pravdíková K, Kolbabová L, Hergeselová T, Hašková P, Jansová H, Macháček M, Jirkovská A, Richardson V, Lane DJR, Kalinowski DS, Richardson DR, Vávrová K, Šimůnek T. Structure-activity relationships of novel salicylaldehyde isonicotinoyl hydrazone (SIH) analogs: iron chelation, anti-oxidant and cytotoxic properties. PLOS One. 2014; 9(11):e112059

2013

Školová B, Janůšová B, Zbytovská J, Gooris G, Bouwstra JA, Slepička P, Berka P, Roh J, Palát K, Hrabálek A, Vávrová K. Ceramides in the Skin Lipid Membranes: Length Matters. Langmuir 2013, 29(50):15624-15633.

Janůšová B, Školová B, Tükörová K, Wojnarová L, Šimůnek T, Mladěnka P, Filipský T, Říha M, Roh J, Palát K, Hrabálek A, Vávrová K. Amino acid derivatives as transdermal permeation enhancers. J. Control. Release 2013, 165(2):91-100.

Karabanovich G, Roh J, Padělková Z, Novák Z, Vávrová K, Hrabálek A. One-pot synthesis of 1-substituted-5-alkylselanyl-1H-tetrazoles from isoselenocyanates: unexpected formation of N-alkyl-N-arylcyanamides and (Z)-Se-alkyl-N-cyano-N,N′-diarylisoselenoureas. Tetrahedron 2013, 69(41):8798-8808.

Filipský T, Říha M, Hrdina R, Vávrová K, Mladěnka P, Mathematical calculations of iron complex stoichiometry by direct UV-Vis spectrophotometry. Bioorg. Chem. 2013, 49:1-8.

2012

Roh J, Vávrová K, Hrabálek A. Synthesis and Functionalization of 5-Substituted Tetrazoles. Eur. J. Org. Chem. 2012, 31:6101-6118.

Macková E, Hrušková K, Bendová P, Vávrová A, Jansová H, Hašková P, Kovaříková P, Vávrová K, Šimůnek T. Methyl and ethyl ketone analogs of salicylaldehyde isonicotinoyl hydrazone: Novel iron chelators with selective antiproliferative action. Chem.-Biol. Interact. 2012; 197(2–3):69-79.

Stariat J, Šesták V, Vávrová K, Nobilis M, Kollárová Z, Klimeš J, Kalinowski DS, Richardson DR, Kovaříková P. LC-MS/MS identification of the principal in vitro and in vivo phase I metabolites of the novel thiosemicarbazone anti-cancer drug, Bp4eT. Anal. Bioanal. Chem. 2012, 403(1):309-321.

2011

Vávrová K, Kovaříková P, Školová B, Líbalová M, Roh J, Čáp R, Holý A, Hrabálek A. Enhanced topical and transdermal delivery of antineoplastic and antiviral acyclic nucleoside phosphonate cPr‑PMEDAP. Pharm. Res. 2011; 28(12):3105-3115.

Janůšová B, Zbytovská J, Lorenc P, Vavrysová H, Palát K, Hrabálek A, Vávrová K. Effect of ceramide acyl chain length on skin permeability and thermotropic phase behavior of model stratum corneum lipid membranes. Biochim. Biophys. Acta Mol. Cell Biol. Lipids 2011; 1811(3):129-137.

Novotný M, Klimentová J, Janůšová B, Palát K. Hrabálek A, Vávrová K. Ammonium carbamates as highly active transdermal permeation enhancers with a dual mechanism of action. J. Control. Release 2011; 150(2):164-70.

Žebrakovská I, Máša M, Srp J, Horn M, Vávrová K, Mareš M. Complex modulation of peptidolytic activity of cathepsin D by sphingolipids. Biochim. Biophys. Acta Mol. Cell Biol. Lipids 2011; 1811(12):1097-1104.

Vavříková E, Polanc S, Kočevar M, Horváti K, Bősze S, Stolaříková J, Vávrová K, Vinšová J. New fluorine-containing hydrazones active against MDR-tuberculosis. Eur. J. Med. Chem. 2011, 46(10):4937-45.

Hašková P, Koubková L, Vávrová A, Macková E, Hrušková K, Kovaříková P, Vávrová K, Šimůnek T. Comparison of various iron chelators used in clinical practice as protecting agents against catecholamine-induced oxidative injury and cardiotoxicity. Toxicology 2011, 289(2-3):122-131.

Hrušková K, Kovaříková P, Bendová P, Hašková P, Macková E, Stariat J, Vávrová A, Vávrová K, Šimůnek T. Synthesis and initial in vitro evaluations of novel antioxidant aroylhydrazone iron chelators with increased stability against plasma hydrolysis. Chem. Res. Toxicol. 2011; 24(3):290-302.

Kovaříková P, Stariat J, Klimeš J, Hrušková K, Vávrová K. Hydrophilic interaction liquid chromatography in the separation of a moderately lipophilic drug from its highly polar metabolites - the cardioprotectant dexrazoxane as a model case. J. Chrom. A 2011; 1218(3):416-426.

2010

Novotný M, Hrabálek A, Janůšová B, Novotný J, Vávrová K. Transkarbams as transdermal permeation enhancers; Effects of ester position and ammonium carbamate formation. Bioorg. Med. Chem. Lett. 2010; 20(9): 2726-8.

Novotný J, Hrabálek A, Vávrová K. Synthesis and Structure-Activity Relationships of Skin Ceramides. Curr. Med. Chem. 2010; 17(21):2301-24.

Bendova P, Mackova E, Haskova P, Vavrova A, Jirkovsky E, Sterba M, Popelova O, Kalinowski DS, Kovarikova P, Vavrova K, Richardson DR, Simunek T. Comparison of clinically used and experimental iron chelators for protection against oxidative stress-induced cellular injury. Chem. Res. Toxicol. 2010; 23(6):1105-14.

Roh J, Vávrová K, Hrabálek A. One-pot regioselective vinylation of tetrazoles: preparation of 5-substituted 2-vinyl-2H-tetrazoles. Tetrahedron Lett. 2010; 51(10):1411-4.

Ferriz JM, Vávrová K, Kunc F, Imramovský A, Stolaříková J, Vavříková E, Vinšová J. Salicylanilide carbamates: Antitubercular agents active against multidrug-resistant Mycobacterium tuberculosis strains. Bioorg. Med. Chem. 2010; 18(3):1054-61.

2009

Novotný J, Pospěchová K, Hrabálek A, Čáp R, Vávrová K. Synthesis of Fluorescent C24-Ceramide: Evidence for Acyl Chain Length Dependent Differences in Penetration of Exogenous NBD-Ceramides into Human Skin. Bioorg. Med. Chem. Lett. 2009; 19(24): 6975-6977.

Zbytovská J, Vávrová K, Kiselev MA, Lessieur P, Wartewig S, Neubert RHH. The effects of transdermal permeation enhancers on thermotropic phase behaviour of a stratum corneum lipid model. Col. Surf. A 2009; 351:30-37.

Novotný M, Hrabálek A, Janůšová B, Novotný J, Vávrová K. Dicarboxylic acid esters as transdermal permeation enhancers: effects of chain number and geometric isomers. Bioorg. Med. Chem. Lett. 2009; 19(2):344-347.

Novotný J, Kovaříková P, Novotný M, Janůšová B, Hrabálek A, Vávrová K. Dimethylamino acid esters as biodegradable and reversible transdermal permeation enhancers: effects of linking chain length, chirality and polyfluorination. Pharm. Res. 2009;26(4):811-21.

Novotný J, Janůšová B, Novotný M, Hrabálek A, Vávrová K. Short-Chain Ceramides Decrease Skin Barrier Properties. Skin Pharmacol. Physiol. 2009; 22(1):22-30.

Roh J, Artamonova TV, Vávrová K, Koldobskii GI, Hrabálek A. Practical Synthesis of 5-Substituted Tetrazoles under Microwave Irradiation. Synthesis 2009; 13: 2175-2178.

2008

Vávrová K, Lorencová K, Novotný J, Holý A, Hrabálek A. Permeation enhancer dodecyl 6-(dimethylamino)hexanoate increases transdermal and topical delivery of adefovir; influence of pH, ion-pairing and skin species. Eur. J. Pharm. Biopharm. 2008; 70(3):901-7.

Klimentová J, Kosák P, Vávrová K, Holas T, Novotný J, Hrabálek A. Transkarbams with Terminal Branching as Transdermal Permeation Enhancers. Bioorg. Med. Chem. Lett. 2008; 18(5):1712-1715.

Vávrová K, Lorencová K, Klimentová J, Novotný J, Holý A, Hrabálek A. Transdermal and dermal delivery of adefovir; effects of pH and permeation enhancers. Eur. J. Pharm. Biopharm. 2008; 69(2):597-604.

Kovaříková P, Vávrová K, Tomalová K, Schöngut M, Hrušková K, Hašková P, Klimeš J. HPLC-DAD and MS/MS analysis of novel drug candidates from the group of aromatic hydrazones revealing the presence of geometric isomers. J. Pharm. Biomed. Anal. 2008; 48(2):295-302.

2007

Vávrová K, Hrabálek A, Mac-Mary S, Humbert P, Muret P. Ceramide analogue 14S24 selectively recovers perturbed human skin barrier. Brit. J. Dermatol. 2007; 157 (4):704-712.

Vávrová K, Lorencová K, Klimentová J, Novotný J, Hrabálek A. HPLC method for determination of in vitro delivery through and into porcine skin of adefovir (PMEA). J. Chromatogr. B. 2007; 853(1-2):198-203.

Hrabálek A, Vávrová K. Historie a současný stav ve vývoji akcelerantů transdermální permeace na Farmaceutické fakultě UK v Hradci Králové – od esterů 6-aminohexanové kyseliny k transkarbamům (II. část). Praktické lékárenství 2007; 3(6):299-300.

Hrabálek A, Doležal P, Vávrová K. Historie a současný stav ve vývoji akcelerantů transdermální permeace na Farmaceutické fakultě UK v Hradci Králové – od esterů 6-aminohexanové kyseliny k transkarbamům (I. část). Praktické lékárenství 2007; 3(2):90-2.

2006

Klimentová J, Kosák P, Vávrová K, Holas T, Hrabálek A. Influence of terminal branching on the transdermal permeation-enhancing activity in fatty alcohols and acids. Bioorg. Med. Chem. 2006; 14(23):7681-7.

Holas T, Vávrová K, Šíma M, Klimentová J, Hrabálek A. Synthesis and transdermal permeation-enhancing activity of carbonate and carbamate analogs of Transkarbam 12. Bioorg. Med. Chem. 2006; 14(23):7671-80.

Hrabálek A, Doležal P, Vávrová K, Zbytovská J, Holas T, Klimentová J, Novotný J. Synthesis and Enhancing Effect of Transkarbam 12 on the Transdermal Delivery of Theophylline, Clotrimazole, Flobufen, and Griseofulvin. Pharm. Res. 2006; 23(5):912-8.

Klimentová J, Hrabálek A, Vávrová K, Holas T, Kroutil A. Synthesis and transdermal penetration-enhancing activity of carbonic and carbamic acid esters-comparison with transkarbam 12. Bioorg. Med. Chem. Lett. 2006; 16(7):1981-4.

Holas T, Vávrová K, Klimentová J, Hrabálek A. Synthesis and transdermal permeation-enhancing activity of ketone, amide, and alkane analogs of Transkarbam 12. Bioorg. Med. Chem. 2006; 14(9):2896-2903.

Kovaříková P, Mokrý M, Klimeš J, Vávrová K. HPLC study on stability of pyridoxal isonicotinoyl hydrazone. J. Pharm. Biomed. Anal. 2006; 40(1):105-12.

Holas T, Zbytovská J, Vávrová K, Berka P, Mádlová M, Klimentová J, Hrabálek A. Thermotropic phase behavior of long-chain alkylammonium-alkylcarbamates. Thermochim. Acta 2006; 441(2):116-123

Vávrová K, Hrabálek A. Role ceramidů v kůži. Praktické lékárenství 2006; 2:55-58.

2005

Vávrová K, Zbytovská J, Hrabálek A. Amphiphilic transdermal permeation enhancers: structure-activity relationships. Curr. Med. Chem. 2005; 12(19):2273-91.

Hrabálek A, Vávrová K, Doležal P, Macháček M. Esters of 6-aminohexanoic acid as skin permeation enhancers: The effect of branching in the alkanol moiety. J. Pharm. Sci. 2005; 94(7):1494-9.

Vávrová K, Hrabálek A, Doležal P, Holas T, Klimentová J. Biodegradable derivatives of tranexamic acid as transdermal permeation enhancers. J. Control. Release. 2005; 104(1):41-9.

Hrabálek A, Vávrová K. Lze překonat kožní bariéru? Praktické lékárenství 2005; 1:8-11.

2004

Kovaříková P, Mokrý M, Klimeš J, Vávrová K. Chromatographic methods for the separation of biocompatible iron chelators from their synthetic precursors and iron chelates. J. Sep. Sci. 2004; 27(17-18):1503-10.

Vávrová K, Zbytovská J, Palát K, Holas T, Klimentová J, Hrabálek A, Doležal P. Ceramide analogue 14S24 ((S)-2-tetracosanoylamino-3-hydroxypropionic acid tetradecyl ester) is effective in skin barrier repair in vitro. Eur. J. Pharm. Sci. 2004; 21(5):581-7.

Hrabálek A, Myznikov L, Kuneš J, Vávrová K, Koldobskii G. A simple method for the preparation of 5-alkylsulfinyl-1-aryltetrazoles. Tetrahedron Lett. 2004; 45(42):7955-7.

2003

Vávrová K, Hrabálek A, Doležal P, Šámalová L, Palát K, Zbytovská J, Holas T, Klimentová J. Synthetic ceramide analogues as skin permeation enhancers: structure-activity relationships. Bioorg. Med. Chem. 2003; 11(24):5381-90.

Vávrová K, Hrabálek A, Doležal P, Holas T, Zbytovská J. L-Serine and glycine based ceramide analogues as transdermal permeation enhancers: polar head size and hydrogen bonding. Bioorg. Med. Chem. Lett. 2003; 13(14):2351-3.

Doležal P, Hrabálek A, Svozil M, Vávrová K, Zbytovská J. In vitro studies on transkarbam 12 and laurocapram effect on transdermal permeation of butorphanol. Farmacevtski Vestnik 2003; 54 (Spec. Iss.):527-8

2002

Vávrová K, Hrabálek A, Doležal P. Enhancement effects of (R) and (S) enantiomers and the racemate of a model enhancer on permeation of theophylline through human skin. Arch. Dermatol. Res. 2002; 294(8):383-5.