Our Charles University Faculty of Pharmacy and its Department of Biochemical Sciences provide us with good technical background and core instruments, including several deep freezers, cell line cryopreservation facility, spectrophotometers and fluorimeters including the versatile Tecan Infinite 200 M and Tecan SPARK plate readers, various centrifuges and ultracentrifuges as well as radioactivity and GMO (class II)-accredited laboratories.
Our cell culture laboratory enables experiments with both permanent cell lines and isolations of primary cell cultures, such as the cardiomyocytes. We have two biohazard class II laminar flow cabinets, CO2 and CO2/N2 incubators, Newport research arc lamp source 450-1000W Xe-ozone free lamp (for photodynamic experiments) and an routine inverted epifluorescence microscope (Nikon Eclipse TS100) with a digital cooled CCD camera and NIS Elements 3.10 software (Laboratory Imaging). We recently obtained new Lonza 4D Nucleofector device equipped with the Y-unit for transfection of adherent primary cells and have access to the Accuri C6 flow cytometer.
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| Cell culture laboratory |
450-1000 W Xe ozon-free lamp (Newport) for PDT experiments |
For advanced microscopical methods we use Nikon A1+ confocal system (based on Nikon Eclipse Ti-E fully-integrated and motorized inverted microscope) with Perfect focus System, highly sensitive cooled sCMOS digital camera Andor Zyla 5.5, NIS Elements Ar 4.2 software (Laboratory Imaging), CoolLED fluorescence source and Stage Top CO2 Incubator.
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| Biohazard class II laminar flow cabinets and CO2 incubator in cell culture laboratory |
Nikon Eclipse TS100 epifluorescence microscope |
Lonza 4D Nucleofector (equipped with Y-Unit) and shaking incubator |
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| CO2 incubator |
CO2/N2 incubator |
CO2/N2 incubator - divided inner glass door |