Research focus
- Pharmacology and toxicology of the cardiovascular system with emphasis in the role of platelets in the onset and development of cardiovascular conditions and the physiological processes involved
- Study of the effect of contaminants and natural compounds on therapeutic targets, particularly on nuclear receptors
Education
2019
PharmDr. in Pharmacology Department of Pharmacology and Toxicology, Faculty of Pharmacy in Hradec Králové, Charles University (Czech Republic).
2017
Ph.D. study in Pharmacology, Department of Pharmacology and Toxicology, Faculty of Pharmacy in Hradec Králové, Charles University (Czech Republic). Thesis: „Nuclear receptors – new ligands study and importance of gene variability“. Supervisor: prof. Petr Pávek, Ph.D.
2013
Degree in Pharmacy, Faculty of Pharmacy, University of Alcalá (Alcalá de Henares, Madrid, Spain)
Employment
09.2019 – present
Assistant professor, Department of Pharmacology and Toxicology, Faculty of Pharmacy in Hradec Králové, Charles University (Czech Republic)
09.2017 – 08.2019
Postdoc, Institute of Molecular and Translational Medicine, Faculty of Medicine and Dentistry, Palacký University (Olomouc, Czech Republic)
10.2013 – 08.2017
Junior researcher, Department of Pharmacology and Toxicology, Faculty of Pharmacy in Hradec Králové, Charles University (Czech Republic)
Teaching activities
2022/23 - present
Lecturer and examiner of Pharmacology I and II for M.Sc. students, Czech and English languages
2020/21 - present
Guarantor of new optional subject „Experimental Pharmacodynamics“ – 4th year of Pharmacy Master Degree
2019/20 - present
Lecturer and examiner of Toxicology for M.Sc. students, Czech and English languages
Student mentorship (2019 – present):
- Diploma thesis:
- 4 defended (1 granted as Rigorous thesis); 3 in CZ, 1 in EN
- 3 ongoing
- Rigorous thesis:
- 1 ongoing (2022 - present)
- SOČ:
- 1 defended (2023, reached national round)
- 1 ongoing
- Erasmus + students mentor (3 students)
Research stages
09.-11.2022
Laboratory of Prof. Marc Freichel, MD.Ph.D., Institute of Pharmacology, Faculty of Medicine, University of Heidelberg, Germany
04-06.2016
Laboratory of Prof. S. Mani, MD., Ph.D., Albert Einstein School of Medicine, Yeshiva University, Bronx, New York City, New York, USA
09.2011-06.2012
ERASMUS Scientific stage at the Department of Organic and Bioorganic Chemistry, Faculty of Pharmacy in Hradec Králové, Charles University. Supervisor: Prof. Jarmila Vinšová, CSc.
Grants
As main researcher:
- 2021-2024: AZV - Czech Health Research Council (NU21J-02-00021): Differences in parameters of platelets aggregation and blood coagulation between healthy individuals and patients with metabolic diseases
- 2022 DAAD (Deutscher Akademischer Austauschdienst- German Academic Exchange Service)
- 2022 Fond Mobility - FM/c/2022-1-009
- 2016 Fond Mobility
As a team member:
- 2024-2026: GAČR Lead Agency (24-12943K) "Bisphenol A substitutes: health risks in relation to the endocrine and cardiovascular systems"
- 2021-2024: AZV - Czech Health Research Council (NU21-02-00135) "Cardiovascular effects of flavonoid metabolites and the impact of metabolic risk factors"
- 2020-2023: Erasmus+ project: Strategic partnership for higher education (2020-1-CZ01-KA203-078218) "Open access Educational Materials On Naturally Occurring Molecules – sources, biological activity and use (OEMONOM)"
- 2x Grant Agency Charles University (GAUK)
Languages
- Spanish: native speaker
- English: fluent, C1 (CAE exam - Cambridge University)
- Czech: fluent
Publication activity
36 papers in IF journals (7 as 1st author, 5 as corresponding author"*"), 1 book chapter
Orcid No.: https://orcid.org/0000-0002-3604-7981
Scopus author ID: 56506910600
WoS author ID: AAC-9590-2019
H-index (Scopus/WoS): 16/16; Total IF: 152.096; ø IF: 4.473; Total citations: > 600
2023
Head-to-head ex vivo comparison of clinically used direct anticoagulant drugs. Fadraersada J, Alva-Gallegos R, Skořepa P, Musil F, Javorská L, Matoušová K, Krčmová LK, Paclíková M, Carazo A, Blaha V, Mladěnka P. Naunyn Schmiedebergs Arch Pharmacol. 2023 Dec 19. doi: 10.1007/s00210-023-02891-x. Epub ahead of print. PMID: 38112731. IF2022:3.6; Q2
Analysis of vitamin K1 and major K2 variants in rat/human serum and lipoprotein fractions by a rapid, simple, and sensitive UHPLC-MS/MS method. Mrštná K, Matoušová K, Krčmová LK, Carazo A, Pourová J, Mladěnka P, Matysová L, Švec F. J Chromatogr A. 2023 Nov 29;1714:464548. doi: 10.1016/j.chroma.2023.464548. Epub ahead of print. PMID: 38043166. IF2022:4.1; Q2
Toxicity overview of endocrine disrupting chemicals interacting in vitro with the oestrogen receptor. Alva-Gallegos R, Carazo A, Mladěnka P. Environ Toxicol Pharmacol. 2023 Apr;99:104089. doi: 10.1016/j.etap.2023.104089. Epub 2023 Feb 24. PMID: 36841273. IF2022:4.5; Q2
2-Substituted quinazolines: Partial agonistic and antagonistic ligands of the constitutive androstane receptor (CAR). Brožová ZR, Dušek J, Palša N, Maixnerová J, Kamaraj R, Smutná L, Matouš P, Braeuning A, Pávek P, Kuneš J, Gathergood N, Špulák M, Pour M, Carazo A*. Eur J Med Chem. 2023 Jul 13;259:115631. doi: 10.1016/j.ejmech.2023.115631. Online ahead of print.PMID: 37473690. IF2022:7.088; Q1, D1
The Impact of Convertase Subtilisin/Kexin Type 9 Monoclonal Antibodies with and without Apheresis on Platelet Aggregation in Familial Hypercholesterolemia. Konečný L, Hrubša M, Karlíčková J, Carazo A, Javorská L, Matoušová K, Krčmová LK, Blaha V, Bláha M, Mladěnka P. Cardiovasc Drugs Ther. 2023 May 2. doi: 10.1007/s10557-023-07455-y. PMID: 37129685. IF2022:3.947; Q2
The Effect of 4-Methylcatechol on Platelets in Familial Hypercholesterolemic Patients Treated with Lipid Apheresis and/or Proprotein Convertase Subtilisin Kexin 9 Monoclonal Antibodies. Konečný L, Hrubša M, Karlíčková J, Carazo A, Javorská L, Matoušová K, Krčmová LK, Šmahelová A, Blaha V, Bláha M, Mladěnka P. Nutrients. 2023 Apr 11;15(8):1842. doi: 10.3390/nu15081842. PMID: 37111061. IF2022:6.706; Q1
Toxicity overview of endocrine disrupting chemicals interacting in vitro with the oestrogen receptor. Alva-Gallegos R, Carazo A*, Mladěnka P. Environ Toxicol Pharmacol. 2023 Feb 23:104089. doi: 10.1016/j.etap.2023.104089. Epub ahead of print. PMID: 36841273. IF2022=5.785; Q2
SC-560 and mofezolac isosteres as new potent COX-1 selective inhibitors with antiplatelet effect. Sisa M, Konečný L, Temml V, Carazo A, Mladěnka P, Landa P. Arch Pharm (Weinheim). 2023 Feb 11:e2200549. doi: 10.1002/ardp.202200549. Epub ahead of print. PMID: 36772878. IF2022=4.613; Q2
The effects of bisphenols on the cardiovascular system ex vivo and in vivo. Tvrdý V, Dias P, Nejmanová I, Carazo A, Jirkovský E, Pourová J, Fadraersada J, Moravcová M, Peterlin Mašič L, Sollner Dolenc M, Mladěnka P. Chemosphere. 2023 Feb;313:137565. doi: 10.1016/j.chemosphere.2022.137565. Epub 2022 Dec 14. PMID: 36528156. IF2022=8.943; Q1
Sex-Related Differences in Platelet Aggregation: A Literature Review Supplemented with Local Data from a Group of Generally Healthy Individuals. Carazo A, Hrubša M, Konečný L, Skořepa P, Paclíková M, Musil F, Karlíčková J, Javorská L, Matoušová K, Krčmová LK, Parvin MS, Šmahelová A, Blaha V, Mladěnka P.Semin Thromb Hemost. 2022 Oct 7. doi: 10.1055/s-0042-1756703. Epub ahead of print. PMID: 36206768. IF2022=6.398; Q1
2022
The Antiplatelet Effect of 4-Methylcatechol in a Real Population Sample and Determination of the Mechanism of Action. Hrubša M, Konečný L, Paclíková M, Parvin MS, Skořepa P, Musil F, Karlíčková J, Javorská L, Matoušová K, Krčmová LK, Carazo A, Šmahelová A, Blaha V, Mladěnka P. Nutrients. 2022 Nov 13;14(22):4798. doi: 10.3390/nu14224798. PMID: 36432485; PMCID: PMC9694226. IF2021=6.706; Q1
Can Isoquinoline Alkaloids Affect Platelet Aggregation in Whole Human Blood? Parvin MS, Hrubša M, Fadraersada J, Carazo A, Karlíčková J, Cahlíková L, Chlebek J, Macáková K, Mladěnka P. Toxins (Basel). 2022 Jul 15;14(7):491. doi: 10.3390/toxins14070491. PMID: 35878229; PMCID: PMC9324755. IF2021=5.075; Q1
Comparison of Antiplatelet Effects of Phenol Derivatives in Humans. Hrubša M, Alva R, Parvin MS, Macáková K, Karlíčková J, Fadraersada J, Konečný L, Moravcová M, Carazo A, Mladěnka P. Biomolecules. 2022 Jan 12;12(1):117. doi: 10.3390/biom12010117. PMID: 35053265; PMCID: PMC8774223. IF2021=4.879; Q2
Screening of Synthetic Heterocyclic Compounds as Antiplatelet Drugs. Hrubša M, Nurjamal K, Carazo A, Nayek N, Karlíčková J, Applová L, Karmakar I, Parvin S, Fadraersada J, Macáková K, Mladěnka P, Brahmachari G. Med Chem. 2022;18(5):536-543. doi: 10.2174/1573406417666211026150658. PMID: 34702153. IF2021=2.745; Q3
Vitamin K - sources, physiological role, kinetics, deficiency, detection, therapeutic use, and toxicity. Mladěnka P, Macáková K, Kujovská Krčmová L, Javorská L, Mrštná K, Carazo A, Protti M, Remião F, Nováková L; OEMONOM researchers and collaborators. Nutr Rev. 2022 Mar 10;80(4):677-698. doi: 10.1093/nutrit/nuab061. PMID: 34472618; PMCID: PMC8907489. IF2021=7.110; Q1
2021
Laboratory Techniques in Cellular and Molecular Medicine (1st ed., 2021). Carazo, A. (Chapter 42; p. 325-341). Palacký University Olomouc. https://doi.org/10.5507/lf.22.24460499;
Triazolo Based-Thiadiazole Derivatives. Synthesis, Biological Evaluation and Molecular Docking Studies. Kamoutsis C, Fesatidou M, Petrou A, Geronikaki A, Poroikov V, Ivanov M, Soković M, Ćirić A, Carazo A, Mladěnka P. Antibiotics (Basel). 2021 Jul 2;10(7):804. doi: 10.3390/antibiotics10070804. PMID: 34356726; PMCID: PMC8300616; IF2020=4,639; Q2
Chromenol Derivatives as Novel Antifungal Agents: Synthesis, In Silico and In Vitro Evaluation. Zveaghintseva M, Stingaci E, Pogrebnoi S, Smetanscaia A, Valica V, Uncu L, Ch Kravtsov V, Melnic E, Petrou A, Glamočlija J, Soković M, Carazo A, Mladěnka P, Poroikov V, Geronikaki A, Macaev FZ. Molecules. 2021 Jul 16;26(14):4304. doi: 10.3390/molecules26144304. PMID: 34299579; PMCID: PMC8307147; IF2020=4,411; Q2
Vitamin A Update: Forms, Sources, Kinetics, Detection, Function, Deficiency, Therapeutic Use and Toxicity. Carazo A*, Macáková K, Matoušová K, Krčmová LK, Protti M, Mladěnka P. Nutrients. 2021 May 18;13(5):1703. doi: 10.3390/nu13051703. PMID: 34069881; PMCID: PMC8157347; IF2020=5,717; Q1
Synthesis of 3,3-dimethyl-6-oxopyrano[3,4-c]pyridines and their antiplatelet and vasodilatory activity. Sirakanyan SN, Hrubša M, Spinelli D, Dias P, Kartsev V, Carazo A, Hovakimyan AA, Pourová J, Hakobyan EK, Karlíčková J, Parvin S, Fadraersada J, Macáková K, Geronikaki A, Mladěnka P. J Pharm Pharmacol. 2021 Jun 9:rgab075. doi: 10.1093/jpp/rgab075. Epub ahead of print. PMID: 34106261; IF2020=3,765; Q2
5-Benzyliden-2-(5-methylthiazol-2-ylimino)thiazolidin-4-ones as Antimicrobial Agents. Design, Synthesis, Biological Evaluation and Molecular Docking Studies. Haroun M, Tratrat C, Kolokotroni A, Petrou A, Geronikaki A, Ivanov M, Kostic M, Sokovic M, Carazo A, Mladěnka P, Sreeharsha N, Venugopala KN, Nair AB, Elsewedy HS. Antibiotics (Basel). 2021 Mar 17;10(3):309. doi: 10.3390/antibiotics10030309. PMID: 33802949; PMCID: PMC8002837; IF2020=4,639; Q2
2020
Interaction of 2,6,7-trihydroxy-xanthene-3-ones with iron and copper, and biological effect of the most active derivative on breast cancer cells and erythrocytes. Mladěnka, P; Karlíčková, J.; Hrubša, M.; Veljović, E.; Muratović, S.; Carazo, A.; Mali, A.S.; Špirtović-Halilović, S.; Saso, L.; Pour, M.; Durić, K. Applied Sciences 2020; IF2019=2,474; Q2
2019
Marine Ligands of the Pregnane X Receptor (PXR): An Overview. Carazo A, Přemysl Mladěnka, Petr Pávek. Mar Drugs. 2019;17(10):554. Published 2019 Sep 28. doi:10.3390/md17100554
Mar Drugs 2019 - Review. PMID 31569349; IF2018=3,772; Q1
Trans-resveratrol, but not other natural stilbenes occurring in food, carries the risk of drug-food interaction via inhibition of cytochrome P450 enzymes or interaction with xenosensor receptors. Hyrsova L, Vanduchova A, Dusek J, Smutny T, Carazo A, Maresova V, Trejtnar F, Barta P, Anzenbacher P, Dvorak Z, Pavek P. Toxicol Lett. 2019 Jan;300:81-91. doi: 10.1016/j.toxlet.2018.10.028. Epub 2018 Oct 27. PMID:30394306; IF2018=3,499; Q2
2018
Teriflunomide Is an Indirect Human Constitutive Androstane Receptor (CAR) Activator Interacting With Epidermal Growth Factor (EGF) Signaling. Carazo A, Dusek J, Holas O, Skoda J, Hyrsova L, Smutny T, Soukup T, Dosedel M, Pávek P. Front Pharmacol. 2018 Oct 11;9:993. doi: 10.3389/fphar.2018.00993. eCollection 2018. PMID:30364229; IF2017=3,831; Q1
Effect of Endocannabinoid Oleamide on Rat and Human Liver Cytochrome P450 Enzymes in In Vitro and In Vivo Models. Dovrtelova G, Zendulka O, Noskova K, Jurica J, Pes O, Dusek J, Carazo A, Zapletalova I, Hlavacova N, Pavek P. Drug Metab Dispos. 2018 Jun;46(6):913-923. doi: 10.1124/dmd.117.079582. Epub 2018 Apr 12. PMID:29650790; IF2017=3,640; Q1
Unexpected Effects of Propiconazole, Tebuconazole, and Their Mixture on the Receptors CAR and PXR in Human Liver Cells. Knebel C, Neeb J, Zahn E, Schmidt F, Carazo A, Holas O, Pavek P, Püschel GP, Zanger UM, Süssmuth R, Lampen A, Marx-Stoelting P, Braeuning A. Toxicol Sci. 2018 May 1;163(1):170-181. doi: 10.1093/toxsci/kfy026. PMID:29420809; IF2017=4,181; Q1
2017
Resveratrol modifies biliary secretion of cholephilic compounds in sham-operated and cholestatic rats. Dolezelova E, Prasnicka A, Cermanova J, Carazo A, Hyrsova L, Hroch M, Mokry J, Adamcova M, Mrkvicova A, Pavek P, Micuda S. World J Gastroenterol. 2017 Nov 21;23(43):7678-7692. doi: 10.3748/wjg.v23.i43.7678. PMID:29209109; IF2016=3,365; Q2
Iron depletion induces hepatic secretion of biliary lipids and glutathione in rats. Prasnicka A, Cermanova J, Hroch M, Dolezelova E, Rozkydalova L, Smutny T, Carazo A, Chladek J, Lenicek M, Nachtigal P, Vitek L, Pavek P, Micuda S. Biochim Biophys Acta Mol Cell Biol Lipids. 2017 Dec;1862(12):1469-1480. doi: 10.1016/j.bbalip.2017.09.003. Epub 2017 Sep 6. PMID:28888833; IF2016=5,547; Q1
Steviol, an aglycone of steviol glycoside sweeteners, interacts with the pregnane X (PXR) and aryl hydrocarbon (AHR) receptors in detoxification regulation. Dusek J, Carazo A, Trejtnar F, Hyrsova L, Holas O, Smutny T, Micuda S, Pavek P. Food Chem Toxicol. 2017 Nov;109(Pt 1):130-142. doi: 10.1016/j.fct.2017.09.007. Epub 2017 Sep 5. PMID:28887089; IF2016=3,778; Q1
Development of water-soluble 3,5-dinitrophenyl tetrazole and oxadiazole antitubercular agents. Roh J, Karabanovich G, Vlčková H, Carazo A, Němeček J, Sychra P, Valášková L, Pavliš O, Stolaříková J, Klimešová V, Vávrová K, Pávek P, Hrabálek A. Bioorg Med Chem. 2017 Oct 15;25(20):5468-5476. doi: 10.1016/j.bmc.2017.08.010. Epub 2017 Aug 8. PMID:28835350; IF2016=2,930; Q2
S-substituted 3,5-dinitrophenyl 1,3,4-oxadiazole-2-thiols and tetrazole-5-thiols as highly efficient antitubercular agents. Karabanovich G, Němeček J, Valášková L, Carazo A, Konečná K, Stolaříková J, Hrabálek A, Pavliš O, Pávek P, Vávrová K, Roh J, Klimešová V. Eur J Med Chem. 2017 Jan 27;126:369-383. doi: 10.1016/j.ejmech.2016.11.041. Epub 2016 Nov 21. PMID:27907875; IF2016=4,519; Q1
Acetylated deoxycholic (DCA) and cholic (CA) acids are potent ligands of pregnane X (PXR) receptor. Carazo A, Hyrsova L, Dusek J, Chodounska H, Horvatova A, Berka K, Bazgier V, Gan-Schreier H, Chamulitrat W, Kudova E, Pavek P. Toxicol Lett. 2017 Jan 4;265:86-96. doi: 10.1016/j.toxlet.2016.11.013. Epub 2016 Nov 18. PMID:27871908; IF2016=3,858; Q1
2016
2-(3-Methoxyphenyl)quinazoline Derivatives: A New Class of Direct Constitutive Androstane Receptor (CAR) Agonists. Smutny T, Nova A, Drechslerová M, Carazo A, Hyrsova L, Hrušková ZR, Kuneš J, Pour M, Špulák M, Pavek P. J Med Chem. 2016 May 26;59(10):4601-10. doi: 10.1021/acs.jmedchem.5b01891. Epub 2016 May 12. Erratum in: J Med Chem. 2016 Dec 22;59(24):11195. PMID:27145071; IF2015=5,589; Q1
The pregnane X receptor down-regulates organic cation transporter 1 (SLC22A1) in human hepatocytes by competing for ("squelching") SRC-1 coactivator. Hyrsova L, Smutny T, Carazo A, Moravcik S, Mandikova J, Trejtnar F, Gerbal-Chaloin S, Pavek P. Br J Pharmacol. 2016 May;173(10):1703-15. doi: 10.1111/bph.13472. Epub 2016 Apr 8. PMID:26920453; IF2015=5,259; Q1
2015
The Use of the LanthaScreen TR-FRET CAR Coactivator Assay in the Characterization of Constitutive Androstane Receptor (CAR) Inverse Agonists. Carazo A, Pávek P. Sensors (Basel). 2015 Apr 21;15(4):9265-76. doi: 10.3390/s150409265. PMID:25905697; IF2014=2,245; Q1
The impact of C677T and A1298C MTHFR polymorphisms on methotrexate therapeutic response in East Bohemian region rheumatoid arthritis patients. Soukup T, Dosedel M, Pavek P, Nekvindova J, Barvik I, Bubancova I, Bradna P, Kubena AA, Carazo AF, Veleta T, Vlcek J. Rheumatol Int. 2015 Jul;35(7):1149-61. doi: 10.1007/s00296-015-3219-z. Epub 2015 Jan 25. PMID:25618758; IF2014=1,516; Q3
Chrysin, baicalein and galangin are indirect activators of the human constitutive androstane receptor (CAR). Carazo Fernández A, Smutny T, Hyrsová L, Berka K, Pavek P. Toxicol Lett. 2015 Mar 4;233(2):68-77. doi: 10.1016/j.toxlet.2015.01.013. Epub 2015 Jan 24. PMID:25625231; IF2014=3,262; Q1