Research group of the experimental cardiovascular pharmacology

We are a research group studying the drugs acting on cardiovascular system, in particular in vivo but as well in vitro conditions. Are priorities are:

• proffesionalism
• collaboration (see video)
• friendly working environment
• international team - list of accepted foreign students 

Current research topics

• Effects of flavonoids and other polyphenols on the organism in physiological and pathological conditions
• Screening of novel antiplatelet drugs and assessment of the mechanism of action
• Effects of drugs on vascular smooth muscles both ex vivo and in vivo
• In vitro screening of novel iron/copper/zinc chelators (comparison of chelating activity at different pHs, assessment of the abilities of these compounds to inhibit Fenton chemistry leading to highly reactive oxygen species and tissue derangement)

Current staff 

Faculty employees

• Prof. Radomír Hrdina, M.D., Ph.D.
• Assoc. Prof. Přemysl Mladěnka, Pharm.D., Ph.D.
• Assoc. Prof. Kateřina Macáková, Ph.D.
• Jana Pourová, Pharm.D., Ph.D.
• Marie Vopršalová, Pharm.D., Ph.D.
• Jana Karlíčková, Pharm.D., Ph.D.
• Eduard Jirkovský, Pharm.D., Ph.D.
• Alejandro Carazo, Ph.D.

Postgradual students

• Thomas Migkos, MSc.
• Václav Tvrdý, MSc.
• Hrubša Marcel, MSc. 
• Shamima Parvin, MSc. 
• Patricia Dias, MSc. 
• Jaka Fadraersada, MSc.
• Zuzana Lomozová, MSc.
• Monika Moravcová, MSc.

Publications

• see corresponding page(s)

Collaboration

At the faculty

• Department of Pharmaceutical Botany and Ecology
• Department of Biological and Medical Sciences

Internal (in the Czech republic)

• School of Medicine and University Hospital, Institute for Clinical Biochemistry and Diagnostics, Hradec Králové

• Laboratory of Biotransformation, Institute of Microbiology of the Czech Academy of Sciences, Prague

International

• Prof. Luciano Saso, Department of Physiology and Pharmacology “Vittorio Erspamer”, Universita La Sapienza, Rome, Italy
• Dr. Paolo Bovicelli, Institute of Biomolecular Chemistry of CNR, Rome, Italy
• Assoc. Prof. Elisabetta Gavini, Deparment of Chemistry and Pharmacy, University of Sassari, Italy
• Prof. Claudio Pettinari and Prof. Fabio Marchetti, University of Camerino, Italy
• Prof. Des Richardson, Department of Pathology, University of Sydney, Sydney, New South Wales, Australia

Grants

Current grants

• 2018-2020 Czech Science Foundation, principal investigator: Assoc. Prof. Valentová K, co-investigator: Assoc. Prof. Mladěnka P. - THE ROLE OF OPTICALLY PURE FLAVONOLIGNANS IN CARDIOVASCULAR EFFECTS OF SILYMARIN
• 2019-2021 Charles University, principal investigator: Tvrdy V, MSc. - THE INFLUENCE OF BISPHENOLS ON THE CARDIOVASCULAR SYSTEM – IN VITRO AND IN VIVO STUDY
• 2020-2022 Charles  University, principal investigator: Hrubša M, MSc. - TESTING OF POTENTIALLY NOVEL ANTIPLATELET COMPOUNDS USING METABOLITES OF NATURAL PHENOLIC COMPOUNDS AS TEMPLATES

• 2020-2022 Charles  University, principal investigator: Dias P, MSc. - ARTERIAL BLOOD PRESSURE DECREASING EFFECTS OF SMALL PHENOLIC METABOLITES OF FLAVONOIDS: MECHANISTIC EX VIVO AND IN VIVO STUDY

• 2020-2023 ERASMUS+ project 2020-1-CZ01-KA203-078218, principal investigator: Assoc. Prof. Mladěnka P - OPEN ACCESS EDUCATIONAL MATERIALS ON NATURALLY OCCURRING MOLECULES - SOURCES, BIOLOGICAL ACTIVITY AND USE

Finished grants

• 2017-2019 Charles University in Prague, principal investigator: Migkos T, MSc.  - FLAVONOID METABOLITES, THEIR INTERACTION WITH TRANSITION METALS AND PHARMACOKINETICS - final evaluation of the project: exceptionally good
• 2015-2017 ERASMUS+ project 2015-1-ES01-KA203-015957  principal investigator: Prof. Morales AI (Universita of Salamanca, Spain), co-investigators from FaF UK: Assoc. Prof. Mladěnka P, Dr. Pourová J and Dr. Vopršalová M  -  LEARNING TOXICOLOGY THROUGH OPEN EDUCATIONAL RESOURCES - final evaluation of the project: 90%

• 2015-2017 Charles University in Prague, principal investigator: Najmanová I, MSc. / Applová L, MSc.  - EFFECTS OF FLAVONOIDS AND THEIR METABOLITES ON VASCULAR SMOOTH MUSCLE EX VIVO AND IN VIVO

• 2014-2015 Charles University in Prague, principal investigator: Horňasová V
  SCREENING OF COPPER CHELATORS OF NATURAL AND SYNTHETIC ORIGIN

• 2012-2014 Charles University in Prague, principal investigator: Filipský T, MSc. / Říha M, MSc.
  CATECHOLAMINE CARDIOVASCULAR IMPAIRMENT AND ITS MODULATION BY IRON CHELATORS

• 2010 Ministry of Education, Youth and Sports Principal investigator: Zatloukalová Z, MSc.
  IMPROVEMENT OF THEORETICAL EDUCATION OF PHARMACOLOGY BY A PRACTICAL EXAMPLE OF HEART FUNCTION PARAMETERS MEASUREMENT

• 2008 Ministry of Education, Youth and Sports Principal investigator: Prof. Hrdina R, Ph.D.
  INNOVATION OF THE LAB FOR PRACTICAL EDUCATION OF PHARMACOLOGY

• 2007-2009 Charles University in Prague principal investigator: Bobrovová Z , MSc.
  THE EXTENDED STUDY OF INFLUENCE OF IRON CHELATORS IN A MODEL OF ACUTE MYOCARDIAL INFARCTION

• 2007-2009 GAUK principal investigator: Dr. Řeháková Z, MSc. / Macáková K, MSc.
  THE STUDY OF THE INFLUENCE OF PHENOLIC COMPOUNDS ON A MODEL OF ISCHAEMIC-REPERFUSION MYOCARDIAL IMPAIRMENT IN RELATION TO THEIR IRON CHELATING AND ANTIPLATELET ACTIVITY

• 2007 Ministry of Education, Youth and Sports Principal investigator: Bobrovová Z, MSc.
  EXTENSION OF SEMINAR EDUCATION OF THE SUBJECT PHARMACOLOGY BY AN EXPERIMENTAL TASK FROM PHARMACODYNAMICS

• 2007 Ministry of Education, Youth and Sports Principal investigator: Hübl M, MSc.
  INNOVATION OF EDUCATION OF PHARMACOLOGY AND PATHOLOGY BY THE INCORPORATION OF ACUTE MYOCARDIAL INFARCTION MODEL

• 2006-2008 GAUK principal investigator: Vykrutová E / Škrle J / Filipová V
  THE STUDY OF THE INFLUENCE OF IRON CHELATOR LACTOFERRIN ON A MODEL OF ACUTE MYOCARDIAL INFARCTION AND ON HISTOLOGY OF THE SELECTED ORGAN SYSTEMS

• 2005-2006 GAUK principal investigator: Mladěnka P, MSc.
  THE INFLUENCE OF IRON CHELATORS AND FLAVONOIDS ON THE MODEL OF ACUTE MYOCARDIAL INFARCTION.

Experimental works

Doctoral dissertations

• 2020: Catapano MC, MSc. - Screening of novel chelators of microbiogenic metals
• 2019: Applová L, MSc. - The influence of polyphenolic compounds and their metabolites on platelet activation
• 2017: Najmanová I, MSc. - The effect of polyphenolic substances on vascular smooth muscle
• 2015: Říha M, MSc. - Screening of new iron- and copper-chelating substances – in vivo and in vitro studies
• 2013: Filipsky T, MSc. - Screening of iron-chelating substances and their potential use in the therapy of acute myocardial infarction
• 2012: Zatloukalová L, MSc. - Influence of Polyphenolic Substances with Iron Chelating Activity in Model of Acute Myocardial Infarction and Oxidation Stress Induced by Catecholamines

Rigorous theses (for the title Dr.)

successfully defended rigorous theses 

year	candidate	title	supervisor
2020	Hodinová V, MSc.	The ex vivo study of mechanism of vasodilatation effect of 3,4,-dihydroxyphenylacetic acid and 4-methylcatechol	Jana Pourová
	Katiová D, MSc.	Mechanism of the vasorelaxant effect of biochanin A studied ex vivo on isolated rat aorta	Jana Pourová
	Nováková J, MSc.	Zinc - chelating activity of selected novel chelators from 4-acylpyrazol-5-one group	Přemysl Mladěnka
	Šimánková L, MSc.	Study of vasodilatory effects of selected silymarin flavonolignans ex vivo on isolated rats aorta	Jana Pourová
2019	Kříž J, MSc.	Copper chelating potential of iron chelators from the group of aroylhydrazones	Přemysl Mladěnka
	Rohová M, MSc.	Administration of cytostatic drugs and extravasation	Marie Vopršalová
2018	Gorbunovová E, MSc.	Comparison of copper ion chelation by benzoic acids	Jana Karlíčková
	Horáčková M, MSc.	The vasorelaxing effects of selected quercetin metabolites on rat isolated aorta	Marie Vopršalová
	Mocová K, MSc.	Determination of the stoichiometry of the copper complexes with 7,8-dihydroxycoumarins	Přemysl Mladěnka
2017	Herbolt D, MSc.	Development of a novel spectrophotometric method for zinc ions chelatation screening	Přemysl Mladěnka
	Šumpelová K, MSc.	The effect of quercetin and its selected metabolites on isolated aortal rings of rat	Marie Vopršalová
	Tamborová B, MSc.	Assessment of chelation of cupric ions with flavones by use of hematoxylin method	Jana Karlíčková
2016	Horňasová V, MSc.	Copper-chelating properties of isoflavonoids - acknowledged diploma thesis on the base of a publication	Přemysl Mladěnka
2015	Jeřábková M, MSc.	Copper reducing effects of flavones	Jana Karlíčková
	Koblásová A, MSc.	Copper reducing properties of flavanones	Jana Karlíčková
	Kolouch J, MSc.	Assessment of chelating properties of 6,7-dihydroxycoumarin	Tomáš Filipský
	Morkusová M, MSc.	Antiplatelet effects of 5,7-dihydroxy-4-methylcoumarins	Přemysl Mladěnka
	Sedlářová L., MSc.	Analysis of oxidative stress in the model of isoprenaline cardiotoxicity after intravenous administration of rutin	Tomáš Filipský
	Stonawská M, MSc.	Comparison of ferrozine and direct spectrophotometry by assessment of iron chelation	Přemysl Mladěnka
2014	Bechná K, MSc.	Copper chelating properties of 8-hydroxyquinolines	Přemysl Mladěnka
	Linhartová A, MSc.	Oxidative stress in the model of catecholamine cardiotoxicity	Tomáš Filipský
	Michalicová A, MSc.	Assessment of copper chelation of flavones by bathocuproine method	Jana Karlíčková
	Řihošková P, MSc.	Interactions of catechins with copper ions	Jana Karlíčková
2012	Staňová Z, MSc.	The assessment of stoichiometric ratio of the complex chelator-iron by use of the method of Job	Přemysl Mladěnka
	Šulcová M, MSc.	Comparison of iron chelating activity of 8-hydroxyquinolines by direct spectrophotometry	Přemysl Mladěnka
2011	Zárubová P, MSc.	Comparison of iron-chelating effects of clinically used peroral iron chelators and 8-hydroxyquinolines	Přemysl Mladěnka
	Matula A, MSc.	Iron-chelating properties of selected novel chelators from 4-acyl-5-pyrazolone group	Přemysl Mladěnka
	Jarkovská S, MSc.	Assesment of iron chelation by 4-acyl-5-pyrazolones by direct spectrophotometry	Přemysl Mladěnka

 

Experimental diploma theses

new diploma theses

• 2022  (planned year of the defence): Afshari M - Differences in platelet and coagulation responses between healthy persons and patients with metabolic disorders (Dr. Carazo)
• 2022: Elbelová J - Study of the influence of natural drugs on estrogen receptor and confirmation of their effect in cell cultures (Dr. Carazo)  
• 2022: Kadlecová K -  Searching for natural cobalt chelators (Dr. Karlíčková)
• 2022: Nadějová J - Interactions of essential amino acids with iron ions (Dr. Karlíčková)
• 2022: Vyoralová T - Study of the expression and toxicity of 4-methylcatechol derivates in breast cancer cell lines (Dr. Carazo)
• 2022: Nosková K - Search for an efficient cobalt chelator (Assoc. Prof. Mladěnka)        

currently measured diploma theses 

• 2021: Faberová J - Interaction of chalcones with transition metals I. (Assoc. Prof. Macáková)
• 2021: Holotíková N - Can essential amino acids be considered as copper chelators ?  (Dr. Karlíčková)
• 2021: Konečný L - Preparation of a method for detection of the extent of calcium chelation (Assoc. Prof. Mladěnka)
• 2021: Pelnařová K - Search of effective cobalt chelators – 8-hydroxyquinolines (Assoc. Prof. Mladěnka)
• 2021: Sobolová Z - The impact of zinc chelators on alcohol dehydrogenase (Assoc. Prof. Mladěnka)
• 2021: Šulcová M - Interaction of chalcones with transition metals II. (Assoc. Prof. Macáková)
• 2021: Ždárová D - Effects of cobalt-chelating compounds on H9c2 cell line (Dr. Jirkovský)

diploma theses before defense

• 2020: Carbone G (University of Palermo) - Effects of selected bisphenols on H9c2 cell line (Dr. Jirkovský)
• 2020: Dimopoulou T - Interaction of phenylacetic acids with iron, diploma thesis in English (Assoc. Prof. Mladěnka)

 successfully defended diploma theses 

year	student	title of diploma thesis	supervisor(s)
2020	Conte F	Effect of flavonoids on copper trigerred hemolysis, diploma thesis in English, defended the at the University of  Rome 2	Přemysl Mladěnka
	Kladivová A	Chelation of copper ions by thiol containing chelators	Přemysl Mladěnka
	Kreibichová M	Flavonols as zinc chelators and their interaction with alcohol dehydrogenase	Eduard Jirkovský
	Moravcová M	Preparation of a novel method for screeing of cobalt chelators	Přemysl Mladěnka
	Salanciova I	Determination of interaction of reduced and oxidized glutathione with copper, iron and zinc	Jana Karlíčková
	Silva K	Effect of chelators on iron and copper based Fenton Chemistry, diploma thesis  in English via ERASMUS program, defended at the University of Alcalá de Henares	Přemysl Mladěnka / Maria Carmen Catapano
	Slougki T	The effect of silymarin flavonolignans and their sulfated conjugates on blood vessels ex vivo	Jana Pourová
2019	Deriu I	Interactions of selected alkaloids with iron, diploma thesis in English, defended the at the University of  Sassari	Jana Karlíčková & Přemysl Mladěnka
	Fišrová M	The effects of some flavonoids on  isolated rat aortic rings	Marie Vopršalová
	Gutieres C	Effect of chelators on alcohol dehydrogenase, a zinc containing enzyme, diploma thesis in English, defended the at the University of  Lisbon	Přemysl Mladěnka
	Hetman A	Sulfates as Phase II metabolites of natural phenolic compounds (diploma thesis at the Academy of Sciences - consultant: Assoc. Prof. Valentová, in English, proposed also as rigorous thesis )	Přemysl Mladěnka
	Kalinová T	Effect of cholinesterase inhibitors on monoaminergic system and energic metabolism (diploma thesis at 1. Faculty of medicine of CU - consultant: Dr. Hroudová, proposed also as rigorous thesis)	Přemysl Mladěnka
	Klimková K	Determination of the stoichiometry of the copper complexes with dehydrosilybine A (consultant: Assoc. Prof. Valentová, proposed also as rigorous thesis)	Přemysl Mladěnka
	Lomozová Z	Interactions of tamarixetin and isorhamnetin with copper (proposed also as rigorous thesis)	Jana Karlíčková
	Pupalová D	Molecular changes in plateles activated by different inducers	Eduard Jirkovský
	Rajtmajerová I	The in vitro effects of selected fenolic substances on isolated rat vessels (proposed also as rigorous thesis)	Jana Pourová
	Zsáková E	Interference of reducing agents with the hematoxylin assay for determination of cupric chelation (proposed also as rigorous thesis)	Přemysl Mladěnka
2018	Benčová S	Transcriptomic analysis of cutaneous inflammatory biomarkers in a mouse model of small fiber neuropathy (diploma thesis in English, ERASMUS , proposed also as rigorous thesis )	Přemysl Mladěnka
	Beránková A	The in vitro effects of selected substances on isolated rat aorta	Jana Pourová
	Hanuščinová L	Ability of copper chelators to interact with iron and zinc, proposed also as rigorous thesis	Přemysl Mladěnka
	Němcová H	Comparison of interactions of equol and desmethylangolensin with iron and copper, proposed also as rigorous thesis	Jana Karlíčková
	Morávková T	Assessment of the complex stoichiometry of the active dexrazoxane metabolite - ADR-925 with iron and copper	Přemysl Mladěnka / Tomáš Filipský
	Rudišarová S	The effect of quercetin metabolite on the rat isolated arteries	Marie Vopršalová
2017	Gorová B	Iron-chelating properties of fruit extracts of various elderberries	Přemysl Mladěnka / Michal Říha
	Novýsedláková A	The in vitro effects of selected isoflavonoids on isolated rat aorta, acknowledged also as rigorous thesis	Jana Pourová
	Pešková H	Effects of some flavonoids in isolated rat aortic rings	Marie Vopršalová
	Pohanová L	Chelation of ferrous ions by derivatives of xanthene-3-one	Přemysl Mladěnka
	Svobodová M	Microglia control adenosine A2A-receptor mediated astrogliosis - ERASMUS, diploma thesis in English, acknowledged also as rigorous thesis
	Zemanová K	Interaction of phenylpropionic acids with copper, acknowledged also as rigorous thesis	Jana Karlíčková
2016	Babič A	The assesment of interaction of 3, 4- dihydroxyphenylacetic acid, a metabolite of flavonoids,  with iron and copper by use of direct spectrophotometrical approach, diploma thesis in English, defended the at the University of Ljubljana	Přemysl Mladěnka
	Fontana T	Determination of antioxidant capacity of bioactive compounds via Fenton reaction and LC-ED (Determinazione della capacita antiossidante di molecole bioattive mediante reazione di Fenton e LC-ED) - diploma thesis in Italian, performed and defended at the University of Bologna	Laura Mercolini / Přemysl Mladěnka
	Kříž J	Copper chelation by clinically used iron chelators	Přemysl Mladěnka
	Mísař J	Copper reducing effects of quercetin metabolites	Jana Karlíčková
	Mocová K	Determination of the stoichiometry of genistein complex with iron and copper	Tomáš Filipský / Michal Říha
2015	Ambrožová K	Copper reducing effects of isoflavonoids	Jana Karlíčková
	Antonsson M	In vitro analysis of iron and copper chelating activity of phenylacetic acids, metabolites of flavonoids - diploma thesis in English, defended at the University of Gothenburg	Přemysl Mladěnka
	Hloch K	Differences in expression of selected genes between “gambler”and “non-gambler” rats - ERASMUS, diploma thesis in English	Přemysl Mladěnka
	Horňasová V	Copper-chelating properties of isoflavonoids	Přemysl Mladěnka
	Todorovic L	Interaction of baicalein with iron - diploma thesis in English	Přemysl Mladěnka
2014	Jeřábková M	Copper chelating activity of flavonols	Jana Karlíčková
	Liddawieh E	Assessment of the stoichiometry of 3-hydroxyflavone-iron complexes by UV-Vis spectrophotometry - diploma thesis in English	Tomáš Filipský
	Malátová V	Copper reducing activity of flavonols	Přemysl Mladěnka
	Migkos T	The effects of flavonoids on vascular smooth muscles - diploma thesis in English	Přemysl Mladěnka
	Nerat M	The effect of 6,7-dihydroxygroup in flavones on iron chelation - diploma thesis in English, defended at the University of Ljubljana	Přemysl Mladěnka
	Ševčíková A	The assessment of copper chelation by use of direct spectrophotometry	Přemysl Mladěnka
2013	Sedlářová L	Standardization of a method for in vitro assessment of copper chelation	Přemysl Mladěnka
	Morkusová M	Chelation ability of experimentally used iron chelators	Přemysl Mladěnka
2012	Kyjovská V	Comparison of iron reducing properties of flavonols and flavanones	Přemysl Mladěnka
	Břečková A	Iron-chelating properties of selected novel chelators from 4-acyl-5-pyrazolone group II	Přemysl Mladěnka
2011	Šulcová M	Spectrophotometric assessment of iron chelation at different pH	Přemysl Mladěnka
	Zárubová P	Comparison of iron-chelating properties of synthetic iron chelators	Přemysl Mladěnka
2010	Hrabalíková J	The influence of pH on iron chelation	Přemysl Mladěnka
	Jarkovská S	Evaluation of antioxidant activity of hydroxytyrosyl lipophilic esters  – ERASMUS, diploma thesis in English	Přemysl Mladěnka
	Landíková K	Identification of the peptide conjugate with toxic acetaminophen metabolite N-acetyl-p-benzoquinone imine – ERASMUS, diploma thesis in English	Přemysl Mladěnka
2009	Havránková L	Pharmacological screening of substances with iron chelating activity	Přemysl Mladěnka
	Mazurová M	Interrelationship of basic biochemical parameters in patients of type II diabetes	Přemysl Mladěnka