Research group of the experimental cardiovascular pharmacology

We are a research group studying the drugs acting on cardiovascular system, in particular in vivo but as well in vitro conditions. Are priorities are:

Current research topics

  • Effects of flavonoids and other polyphenols and their metabolites on the organism in physiological and pathological conditions
  • Screening of novel antiplatelet drugs and assessment of the mechanism of action
  • Effects of drugs on vascular smooth muscles both ex vivo and in vivo
  • In vitro screening of novel iron/copper/zinc/cobalt chelators (comparison of chelating activity at different pHs, assessment of the abilities of these compounds to inhibit Fenton chemistry leading to highly reactive oxygen species and tissue derangement)

Current staff 

Faculty employees

Postgradual students

  • Václav Tvrdý, MSc.
  • Shamima Parvin, MSc. 
  • Jaka Fadraersada, MSc.
  • Monika Moravcová, MSc.
  • Raul Alva, MSc. 
  • Lukáš Konečný, MSc.
  • Saina Saadat, MSc. 
  • Patrícia Harčárová, MSc. 
  • Catherine Immaculate Gunaseelan, MSc. 
  • Bogdan Adler, MSc. 

Publications

Collaboration

At the faculty

Internal (in the Czech republic)

International

Grants

Current grants

  • 2021-2024 Czech Health Research Council, principal investigator: Prof. Mladěnka P - CARDIOVASCULAR EFFECTS OF FLAVONOID METABOLITES AND THE IMPACT OF METABOLIC RISK FACTORS
  • 2021-2024 Czech Health Research Council - junior project, principal investigator: Dr. Carazo, A - DIFFERENCES IN PARAMETERS OF PLATELETS AGGREGATION AND BLOOD COAGULATION BETWEEN HEALTHY INDIVIDUALS AND PATIENTS WITH METABOLIC DISEASES
  • 2023-2025 Charles University, principal investigator: Saadat S, MSc. - VASODILATORY EFFECTS OF PRINCIPAL HAWTHORN CONSTITUENTS: EX VIVO AND IN VIVO STUDY

  • 2024-2026 Czech Health Research Council & Slovenian Research and Innovation Agency, principal investigator: Dr. Jirkovský, E - Bisphenol A substitutes: health risks to the endocrine and cardiovascular systems

Finished grants

  • 2015-2017 Charles University in Prague, principal investigator: Najmanová I, MSc. / Applová L, MSc.  - EFFECTS OF FLAVONOIDS AND THEIR METABOLITES ON VASCULAR SMOOTH MUSCLE EX VIVO AND IN VIVO
  • 2014-2015 Charles University in Prague, principal investigator: Horňasová V
    SCREENING OF COPPER CHELATORS OF NATURAL AND SYNTHETIC ORIGIN

  • 2012-2014 Charles University in Prague, principal investigator: Filipský T, MSc. / Říha M, MSc.
    CATECHOLAMINE CARDIOVASCULAR IMPAIRMENT AND ITS MODULATION BY IRON CHELATORS

  • 2010 Ministry of Education, Youth and Sports Principal investigator: Zatloukalová Z, MSc.
    IMPROVEMENT OF THEORETICAL EDUCATION OF PHARMACOLOGY BY A PRACTICAL EXAMPLE OF HEART FUNCTION PARAMETERS MEASUREMENT

  • 2008 Ministry of Education, Youth and Sports Principal investigator: Prof. Hrdina R, Ph.D.
    INNOVATION OF THE LAB FOR PRACTICAL EDUCATION OF PHARMACOLOGY

  • 2007-2009 Charles University in Prague principal investigator: Bobrovová Z , MSc.
    THE EXTENDED STUDY OF INFLUENCE OF IRON CHELATORS IN A MODEL OF ACUTE MYOCARDIAL INFARCTION

  • 2007-2009 GAUK principal investigator: Dr. Řeháková Z, MSc. / Macáková K, MSc.
    THE STUDY OF THE INFLUENCE OF PHENOLIC COMPOUNDS ON A MODEL OF ISCHAEMIC-REPERFUSION MYOCARDIAL IMPAIRMENT IN RELATION TO THEIR IRON CHELATING AND ANTIPLATELET ACTIVITY

  • 2007 Ministry of Education, Youth and Sports Principal investigator: Bobrovová Z, MSc.
    EXTENSION OF SEMINAR EDUCATION OF THE SUBJECT PHARMACOLOGY BY AN EXPERIMENTAL TASK FROM PHARMACODYNAMICS

  • 2007 Ministry of Education, Youth and Sports Principal investigator: Hübl M, MSc.
    INNOVATION OF EDUCATION OF PHARMACOLOGY AND PATHOLOGY BY THE INCORPORATION OF ACUTE MYOCARDIAL INFARCTION MODEL

  • 2006-2008 GAUK principal investigator: Vykrutová E / Škrle J / Filipová V
    THE STUDY OF THE INFLUENCE OF IRON CHELATOR LACTOFERRIN ON A MODEL OF ACUTE MYOCARDIAL INFARCTION AND ON HISTOLOGY OF THE SELECTED ORGAN SYSTEMS

  • 2005-2006 GAUK principal investigator: Mladěnka P, MSc.
    THE INFLUENCE OF IRON CHELATORS AND FLAVONOIDS ON THE MODEL OF ACUTE MYOCARDIAL INFARCTION.

Experimental works

Doctoral dissertations

before defence

  • 2024: Tvrdý V, MSc. - Analysis of cardiovascular effects of flavolignans isolated from Silybum marianum and their metabolites

succesfully defended

Rigorous theses (for the title Dr.)

in realization

  • Jandorová E, MSc.: Tvorba agregátů destička-monocyt v kolagenem stimulované lidské krvi ех vivo а vliv preinkubace 4-metylkatecholem (Dr. Carazo)

successfully defended rigorous theses 

year candidate title supervisor
2022 Uramová D, MSc.

The copper - chelating activity of xanthen-3-ones derivates

Přemysl Mladěnka
2021 Gavurová L, MSc. Comparison of interaction of cyanidin and cyanidin-3-glucoside with copper and iron Přemysl Mladěnka
  Kantorová T, MSc.

Copper chelating activity of flavanons

Přemysl Mladěnka
  Kopková N, MSc. Copper reducing properties of a series of xanthen-3-ones Přemysl Mladěnka
  Lavičková B, MSc. Interaction of tamarixetin and isorhamnetin with iron Jana Karlíčková
2020 Hodinová V, MSc. The ex vivo study of mechanism of vasodilatation effect of 3,4,-dihydroxyphenylacetic acid and 4-methylcatechol Jana Pourová
  Katiová D, MSc. Mechanism of the vasorelaxant effect of biochanin A studied ex vivo on isolated rat aorta Jana Pourová
  Nováková J, MSc. Zinc - chelating activity of selected novel chelators from 4-acylpyrazol-5-one group Přemysl Mladěnka
  Šimánková L, MSc. Study of vasodilatory effects of selected silymarin flavonolignans ex vivo on isolated rats aorta Jana Pourová
2019 Kříž J, MSc. Copper chelating potential of iron chelators from the group of aroylhydrazones Přemysl Mladěnka
  Rohová M, MSc.

Administration of cytostatic drugs and extravasation

Marie Vopršalová
2018 Gorbunovová E, MSc. Comparison of copper ion chelation by benzoic acids Jana Karlíčková
  Horáčková M, MSc.

The vasorelaxing effects of selected quercetin metabolites on rat isolated aorta

Marie Vopršalová
  Mocová K, MSc. Determination of the stoichiometry of the copper complexes with 7,8-dihydroxycoumarins Přemysl Mladěnka
2017 Herbolt D, MSc. Development of a novel spectrophotometric method for zinc ions chelatation screening Přemysl Mladěnka
  Šumpelová K, MSc. The effect of quercetin and its selected metabolites on isolated aortal rings of rat Marie Vopršalová
  Tamborová B, MSc. Assessment of chelation of cupric ions with flavones by use of hematoxylin method Jana Karlíčková
2016 Horňasová V, MSc. Copper-chelating properties of isoflavonoids - acknowledged diploma thesis on the base of a publication Přemysl Mladěnka
2015 Jeřábková M, MSc. Copper reducing effects of flavones Jana Karlíčková
  Koblásová A, MSc. Copper reducing properties of flavanones Jana Karlíčková
  Kolouch J, MSc. Assessment of chelating properties of 6,7-dihydroxycoumarin Tomáš Filipský
  Morkusová M, MSc. Antiplatelet effects of 5,7-dihydroxy-4-methylcoumarins Přemysl Mladěnka
  Sedlářová L., MSc. Analysis of oxidative stress in the model of isoprenaline cardiotoxicity after intravenous administration of rutin  Tomáš Filipský
  Stonawská M, MSc. Comparison of ferrozine and direct spectrophotometry by assessment of iron chelation Přemysl Mladěnka
2014 Bechná K, MSc. Copper chelating properties of 8-hydroxyquinolines  Přemysl Mladěnka
  Linhartová A, MSc. Oxidative stress in the model of catecholamine cardiotoxicity Tomáš Filipský
  Michalicová A, MSc. Assessment of copper chelation of flavones by bathocuproine method Jana Karlíčková
  Řihošková P, MSc. Interactions of catechins with copper ions Jana Karlíčková
2012 Staňová Z, MSc. The assessment of stoichiometric ratio of the complex chelator-iron by use of the method of Job Přemysl Mladěnka
  Šulcová M, MSc. Comparison of iron chelating activity of 8-hydroxyquinolines by direct spectrophotometry Přemysl Mladěnka
2011 Zárubová P, MSc. Comparison of iron-chelating effects of clinically used peroral iron chelators and 8-hydroxyquinolines Přemysl Mladěnka
  Matula A, MSc. Iron-chelating properties of selected novel chelators from 4-acyl-5-pyrazolone group Přemysl Mladěnka
  Jarkovská S, MSc. Assesment of iron chelation by 4-acyl-5-pyrazolones by direct spectrophotometry Přemysl Mladěnka

 

Experimental diploma theses

new theses

  • 2025: Heidari Lari Nezhad M - Study of the vasodilatory effects of natural substances in an ex vivo vessel model (Assoc. Prof. Pourová)
  • 2025: Hudáková B – Platelet aggregation in patients suffering from hypercholesterolemia (Prof. Mladěnka)
  • 2025: Kociánová K - Interaction of vitexin and isovitexin with copper (Dr. Lomozová)
  • 2025: Lichnerová L - Platelet aggregation in diabetes mellitus type II patients (Dr. Carazo)
  • 2025: Pavelková Z - Interaction of the major constituents of hawthorn with iron (Dr. Lomozová)
  • 2025: Podlesná K - The impact of metabolites of flavonoids (acetic acids) on the iron-catalysed Fenton reaction (Dr. Lomozová)
  • 2025: Salimiborojeni Y -  Platelet aggregation in geriatric polymorbid patients (Prof. Mladěnka, ERASMUS student)
  • 2025: Toman L - Effect of fatty acids on platelet reactivity  (Dr. Carazo)
  • 2025: Walková M - The impact of small phenolic metabolites of flavonoids (acetic acids) on the copper-catalysed Fenton reaction and hemolysis (Dr. Lomozová)

currently measured diploma theses 

  • 2024 (planned year of the defence):  Gaidosová B - Study of the vasodilatory effect of tamarixetin in an ex vivo porcine coronary vessel model (Assoc. Prof. Pourová)
  • 2024 Hubová N - Study of platelet-monocyte aggregates induction In vitro
  • 2024: Kolářová Z: Evaluation of marine alcaloid fascaplysin as a potential antiplatelet agent (Dr. Carazo)
  • 2024: Joklová J - Aggregation of platelets in patients suffering from diabetes mellitus type I (Prof. Mladěnka)
  • 2024: Mochorovská N - Study of the vasodilatory effect of isorhamnetin in an ex vivo porcine coronary vessel (Assoc. Prof. Pourová)
  • 2024: Spiller S - The impact of small phenolic metabolites of flavonoids (propionic acids) on the iron-catalysed Fenton reaction (Dr. Lomozová)
  • 2024: Streďanská Aneta – Interaction of 7,8-dihydroxyflavone with physiological metals (Prof. Mladěnka)
  • 2023: Wehde M - The effect of selected phenolic metabolites of flavonoids on the copper and iron catalyzed Fenton reaction and copper triggered lysis of erythrocytes (Prof. Mladěnka/ Lomozová MSc., ERASMUS student)

 successfully defended diploma theses 

year student title of diploma thesis supervisor(s)
2023 Argento R Chelation of physiological metals by 3-hydroxyflavone and biological consequences (defence at the University of Bologna, diploma thesis in Italian) Přemysl Mladěnka, Laura Mercolini
  Černotová V The influence of LDL-apheresis on aggregation of platelets, blood coagulation, and the effect of standard drugs Přemysl Mladěnka
  Gardiánová A Characterization of different differentiation protocols of H9c2 cardiomyoblasts Eduard Jirkovský
  Harčárová P The influence of benzoic acids (metabolites of flavonoids) on the copper-triggered Fenton reaction and hemolysis Zuzana Lomozová
  Kašpárková K Search for new natural α-glukosidase inhibitors Jana Karlíčková
  Klenovičová A The impact of benzoic acids, metabolites of flavonoids, on the iron-catalysed Fenton reaction Přemysl Mladěnka, Zuzana Lomozová
  Kleplová D Study of the expression and toxicity of catechol derivates in MCF-7 cell line Alejandro Carazo
  Kuzdřalová K Vasodilatory effects of 4-methyl catechol: ex vivo mechanism of action Jana Pourová
  Macáková B Comparison of the effect of bisphenols on parental and differentiated H9c2 cells Eduard Jirkovský
  Minarovičová J Comparison of platelet aggregation in healthy population Přemysl Mladěnka
  Rotternbornová L The evaluation of senescence markers in the H9c2 cell line after cobalt exposure Eduard Jirkovský
  Skoupilová K Are currently used chelators of heavy metals also chelators of calcium and magnesium? Přemysl Mladěnka
  Štefancová M

Vasodilatory effects of 3-hydroxy phenylacetic acid: ex vivo mechanism of action

Jana Pourová
2022 Afshari M Differences in platelet and coagulation responses between healthy persons and patients with metabolic disorders Alejandro Carazo
  Conti L The effect of selected phenolic metabolites of flavonoids on the copper-catalyzed Fenton reaction and copper-triggered lysis of erythrocytes (in English, defended at the University of Bologna) Přemysl Mladěnka/ Zuzana Lomozová / Laura Mercolini
  Elbelová J Study of the influence of natural drugs on estrogen receptor and confirmation of their effect in cell cultures Alejandro Carazo
  Kadlecová K Searching for natural cobalt chelators Jana Karlíčková, Monika Moravcová
  Nadějová J Interactions of essential amino acids with iron ions Jana Karlíčková
  Nosková K Search for an efficient cobalt chelator Přemysl Mladěnka, Monika Moravcová
  Šmídová K Vasodilatory effects of 3-hydroxyphenylacetic acid ex vivo Jana Pourová
  Vyoralová T Study of the expression and toxicity of 4-methylcatechol derivates in breast cancer cell lines Alejandro Carazo
2021 Faberová J Interaction of chalcones with transition metals I. Kateřina Macáková
  Holotíková N Can essential amino acids be considered as copper chelators ? Jana Karlíčková
  Konečný L Preparation of a method for detection of the extent of calcium chelation Přemysl Mladěnka
  Kuchařová N Vasodilatory effects of bisphenol AF ex vivo Jana Pourová
  Pelnařová K Search of effective cobalt chelators – 8-hydroxyquinolines Přemysl Mladěnka
  Sobolová Z The impact of zinc chelators on alcohol dehydrogenase Přemysl Mladěnka
  Šulcová M Interaction of chalcones with transition metals II Kateřina Macáková
  Ždárová D Effects of cobalt-chelating compounds on H9c2 cell line Eduard Jirkovský
2020 Carbone G  Effects of selected bisphenols on H9c2 cell line, diploma thesis in English, defended the at the University of Palermo Eduard Jirkovský
  Conte F Effect of flavonoids on copper trigerred hemolysis, diploma thesis in English, defended at the University of  Rome 2 Přemysl Mladěnka
  Kladivová A Chelation of copper ions by thiol containing chelators Přemysl Mladěnka
  Kreibichová M Flavonols as zinc chelators and their interaction with alcohol dehydrogenase Eduard Jirkovský
  Moravcová M Preparation of a novel method for screeing of cobalt chelators Přemysl Mladěnka
  Salanciova I Determination of interaction of reduced and oxidized glutathione with copper, iron and zinc Jana Karlíčková
  Silva K Effect of chelators on iron and copper based Fenton Chemistry, diploma thesis  in English via ERASMUS program, defended at the University of Alcalá de Henares Přemysl Mladěnka / Maria Carmen Catapano
  Slougki T The effect of silymarin flavonolignans and their sulfated conjugates on blood vessels ex vivo Jana Pourová
2019 Deriu I Interactions of selected alkaloids with iron, diploma thesis in English, defended the at the University of  Sassari Jana Karlíčková & Přemysl Mladěnka
  Fišrová M

The effects of some flavonoids on  isolated rat aortic rings

Marie Vopršalová
  Gutieres C  Effect of chelators on alcohol dehydrogenase, a zinc containing enzyme, diploma thesis in English, defended the at the University of  Lisbon Přemysl Mladěnka
  Hetman A Sulfates as Phase II metabolites of natural phenolic compounds (diploma thesis at the Academy of Sciences - consultant: Assoc. Prof. Valentová, in English, proposed also as rigorous thesis ) Přemysl Mladěnka
  Kalinová T Effect of cholinesterase inhibitors on monoaminergic system and energic metabolism (diploma thesis at 1. Faculty of medicine of CU - consultant: Dr. Hroudová, proposed also as rigorous thesis) Přemysl Mladěnka
  Klimková K Determination of the stoichiometry of the copper complexes with dehydrosilybine A (consultant: Assoc. Prof. Valentová, proposed also as rigorous thesis) Přemysl Mladěnka
  Lomozová Z Interactions of tamarixetin and isorhamnetin with copper (proposed also as rigorous thesis) Jana Karlíčková
  Pupalová D Molecular changes in plateles activated by different inducers Eduard Jirkovský
  Rajtmajerová I The in vitro effects of selected fenolic substances on isolated rat vessels (proposed also as rigorous thesis) Jana Pourová
  Zsáková E Interference of reducing agents with the hematoxylin assay for determination of cupric chelation (proposed also as rigorous thesis) Přemysl Mladěnka
2018 Benčová S Transcriptomic analysis of cutaneous inflammatory biomarkers in a mouse model of small fiber neuropathy (diploma thesis in English, ERASMUS , proposed also as rigorous thesis ) Přemysl Mladěnka
  Beránková A The in vitro effects of selected substances on isolated rat aorta Jana Pourová
  Hanuščinová L Ability of copper chelators to interact with iron and zinc, proposed also as rigorous thesis  Přemysl Mladěnka
  Němcová H Comparison of interactions of equol and desmethylangolensin with iron and copper, proposed also as rigorous thesis  Jana Karlíčková
  Morávková T Assessment of the complex stoichiometry of the active dexrazoxane metabolite - ADR-925 with iron and copper Přemysl Mladěnka / Tomáš Filipský
  Rudišarová S The effect of quercetin metabolite on the rat isolated arteries Marie Vopršalová
2017 Gorová B Iron-chelating properties of fruit extracts of various elderberries Přemysl Mladěnka / Michal Říha
  Novýsedláková A The in vitro effects of selected isoflavonoids on isolated rat aorta, acknowledged also as rigorous thesis  Jana Pourová
  Pešková H Effects of some flavonoids in isolated rat aortic rings Marie Vopršalová
  Pohanová L Chelation of ferrous ions by derivatives of xanthene-3-one Přemysl Mladěnka
  Svobodová M Microglia control adenosine A2A-receptor mediated astrogliosis - ERASMUS, diploma thesis in English, acknowledged also as rigorous thesis   
  Zemanová K Interaction of phenylpropionic acids with copper, acknowledged also as rigorous thesis  Jana Karlíčková
2016 Babič A The assesment of interaction of 3, 4- dihydroxyphenylacetic acid, a metabolite of flavonoids,  with iron and copper by use of direct spectrophotometrical approach, diploma thesis in English, defended the at the University of Ljubljana Přemysl Mladěnka
  Fontana T Determination of antioxidant capacity of bioactive compounds via Fenton reaction and LC-ED (Determinazione della capacita antiossidante di molecole bioattive mediante reazione di Fenton e LC-ED) - diploma thesis in Italian, performed and defended at the University of Bologna Laura Mercolini / Přemysl Mladěnka
  Kříž J Copper chelation by clinically used iron chelators Přemysl Mladěnka
  Mísař J Copper reducing effects of quercetin metabolites Jana Karlíčková
  Mocová K Determination of the stoichiometry of genistein complex with iron and copper Tomáš Filipský / Michal Říha
2015 Ambrožová K Copper reducing effects of isoflavonoids Jana Karlíčková
  Antonsson M In vitro analysis of iron and copper chelating activity of phenylacetic acids, metabolites of flavonoids - diploma thesis in English, defended at the University of Gothenburg   Přemysl Mladěnka
  Hloch K Differences in expression of selected genes between “gambler”and “non-gambler” rats - ERASMUS, diploma thesis in English  Přemysl Mladěnka
  Horňasová V Copper-chelating properties of isoflavonoids Přemysl Mladěnka
  Todorovic L Interaction of baicalein with iron - diploma thesis in English Přemysl Mladěnka
2014 Jeřábková M Copper chelating activity of flavonols Jana Karlíčková
  Liddawieh E Assessment of the stoichiometry of 3-hydroxyflavone-iron complexes by UV-Vis spectrophotometry - diploma thesis in English  Tomáš Filipský
  Malátová V Copper reducing activity of flavonols Přemysl Mladěnka
  Migkos T The effects of flavonoids on vascular smooth muscles - diploma thesis in English  Přemysl Mladěnka
  Nerat M The effect of 6,7-dihydroxygroup in flavones on iron chelation - diploma thesis in English, defended at the University of Ljubljana Přemysl Mladěnka
  Ševčíková A The assessment of copper chelation by use of direct spectrophotometry Přemysl Mladěnka
2013 Sedlářová L Standardization of a method for in vitro assessment of copper chelation  Přemysl Mladěnka
  Morkusová M Chelation ability of experimentally used iron chelators Přemysl Mladěnka
2012 Kyjovská V Comparison of iron reducing properties of flavonols and flavanones Přemysl Mladěnka
  Břečková A Iron-chelating properties of selected novel chelators from 4-acyl-5-pyrazolone group II Přemysl Mladěnka
2011 Šulcová M Spectrophotometric assessment of iron chelation at different pH Přemysl Mladěnka
  Zárubová P Comparison of iron-chelating properties of synthetic iron chelators Přemysl Mladěnka
2010 Hrabalíková J The influence of pH on iron chelation Přemysl Mladěnka
  Jarkovská S Evaluation of antioxidant activity of hydroxytyrosyl lipophilic esters  – ERASMUS, diploma thesis in English Přemysl Mladěnka
  Landíková K Identification of the peptide conjugate with toxic acetaminophen metabolite N-acetyl-p-benzoquinone imine ERASMUS, diploma thesis in English Přemysl Mladěnka
2009 Havránková L Pharmacological screening of substances with iron chelating activity Přemysl Mladěnka
  Mazurová M Interrelationship of basic biochemical parameters in patients of type II diabetes Přemysl Mladěnka

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