Research focus
- isolation of secondary metabolites from plant families Fumariaceae, Papaveraceae and Amaryllidaceae (isolation, analysis, structural identification)
- in vitro biological activities of isolated compounds (AChE, BuChE, POP, GSK-3β and BACE1 inhibition activity and antioxidative activity)
Education
- 2021 Associate Professor (specialization Pharmacognosy), Charles University in Prague, Faculty of Pharmacy. Habilitation: Isoquinoline alkaloids as potential drugs
- 2007-2012 Ph.D., Charles University in Prague, Faculty of Pharmacy in Hradec Králové, Department of Pharmaceutical Botany and Ecology. Thesis: Study of biological activity of alkaloids isolated from Corydalis cava (Fumariaceae)
- 2002-2007 Master degree, Charles University in Prague, Faculty of Pharmacy. Thesis: Pharmacognostic study of Alnus spp.
Current position
- associate professor at Charles University, Faculty of Pharmacy in Hradec Králové, Department of Pharmaceutical Botany
Teaching activities
- Pharmaceutical botany (practicals)
- Toxicity of natural products (lectures)
- Phytochemical methods (practicals)
- Advamced methods of forensic analysis (lectures, ptacticals)
Research fellowship
- 2011 University of Bologna, Italy, Department of Pharmaceutical Sciences – supervisor prof. Vincenza Andrisano (5 months)
- 2014 Medicinal Chemistry Institute-CSIC in Madrid, Spain – supervisor prof. Ana Martinez (2 months)
- 2018 University of Antwerp, Department of Pharmaceutical Sciences – supervisor Dr. Emmy Tuenter (1 month)
Professional membership
- since 2009 – Czech Chemical Society
- since 2007-2013 – Czech Apothecary Association
Grant recieved
- Chlebek, J., Cahlíková, L., Macáková, K., Opletal, L.: Plant secondary metabolites from subclasses Magnoliidae, Ranunculidae, Cornidae and Lamiidae influencing in vitro activity of human cholinesterases as a contribution to study of Alzheimerʼs disease: bio-guided isolation strategy. Grant Agency of Charles University, No. 122309/2009/B-BIO (2009–2011).
Publications
Monographs (and chapters)
2014
MACÁKOVÁ, K., KOLEČKÁŘ, V., CAHLÍKOVÁ, L., CHLEBEK, J., HOŠŤÁLKOVÁ, A., KUČA, K., JUN, D., OPLETAL, L.: Tannins and their influence on health. Recent Advances in Medicinal Chemistry. Bentham Science Publishers 1, 159-208 (2014).
CAHLÍKOVÁ, L., MACÁKOVÁ, K., BENEŠOVÁ, N., CHLEBEK, J., HOŠŤÁLKOVÁ, A., OPLETAL, L.: Natural compounds (small molecules) as potential and real drugs of Alzehimer’s disease: A critical review, in: Studies in natural products chemistry (ATTA-ur-RAHMAN, ed.), Chapter 6, Elsevier: Amsterdam 2014, pp. 153-194, ISBN: 978-0-444-63281-4.
2010
OPLETAL, L., ROZKOT, M., CHLEBEK, J., ČEŘOVSKÝ, J.: Látky přírodního původu potenciálně ovlivňující reprodukci prasat, in: OPLETAL, L., SKŘIVANOVÁ, V. (eds): Přírodní látky a jejich biologická aktivita. Sv. 2: Využití látek pro ovlivnění fyziologických procesů hospodářských zvířat, s. 517–582, Karolinum, Praha 2010, ISBN 978-80-246-1801-2.
Original papers
2022
FERNANDES, F., BARROSO, F.M., DE SIMONE, A., EMRIKOVÁ, E., DIAS-TEIXEIRA, M., PEREIRA, J.P., CHLEBEK, J., CRUZ-FERNANDES, V., RODRIGUES, F., ANDRISANO, V., DELERUE-MATOS, C., GROSSO, C.: J. Ethnopharmacol. 290, 115107 (2022). J. Ethnopharmacol. 290, 115107 (2022). https://doi.org/10.1016/j.jep.2022.115107
WIJAYA, V., JANDOUREK, O., KROUSTKOVA, J., HRADISKA-BREITEROVA, K., KORABECNY, O., SOBOLOVA, K., KOHELOVA, E., HOSTALKOVA, A., KONECNA, K., SAFRATOVA, M., VRABEC, R., KUNES, J., OPLETAL, O., CHLEBEK, J., CAHLIKOVA, L.: Alkaloids of Dicranostigma franchetianum (Papaveraceae) and berberine derivatives as a novel class of antimycobacterial agents. Biomolecules 12, 844 (2022). https://doi.org/10.3390/biom12060844
VRABEC, R., MAŘÍKOVÁ, J., LOČÁREK, M., KORÁBEČNÝ, J., HULCOVÁ, D., HOŠŤÁLKOVÁ, A., KUNEŠ, J., CHLEBEK, J., KUČERA, T., HRABINOVÁ, M., JUN, D., SOUKUP, O., ANDRISANO, V., JENČO, J., ŠAFRATOVÁ, M., NOVÁKOVÁ, L., OPLETAL, L., CAHLÍKOVÁ, L.: Monoterpene indole alkaloids from Vinca minor L. (Apocynaceae): identification of new structural scaffold for treatment of Alzheimer´s disease. Phytochemistry 194, 113017 (2022). https://doi.org/10.1016/j.phytochem.2021.113017
2021
KOHELOVÁ, E., MAŘÍKOVÁ, J., KORÁBEČNÝ, J., HULCOVÁ, D., KUČERA, T., JUN, D., CHLEBEK, J., JENČO, J., ŠAFRATOVÁ, M., HRABINOVÁ, M., RITOMSKÁ, A., MALANÍK, M., PEŘINOVÁ, R., BREITEROVÁ, K., KUNEŠ, J., NOVÁKOVÁ, L., OPLETAL, L., CAHLÍKOVÁ, L.: Alkaloids of Zephyrantes citrina (Amaryllidaceae) and their implication to Alzheimerʼs disease: isolation, structural elucidation and biological activity. Bioorg. Chem. 107, 104567 (2021). https://doi.org/10.1016/j.bioorg.2020.104567
2019
CHLEBEK, J., KORÁBEČNÝ, J., DOLEŽAL, R., ŠTĚPÁNKOVÁ, Š., PÉREZ, D.I., HOŠŤÁLKOVÁ, A., OPLETAL, L., CAHLÍKOVÁ, L., MACÁKOVÁ, K., KUČERA, T., HRABINOVÁ, M., JUN, D. In vitro and in silico acetylcholinesterase inhibitory activity of thalictricavine and canadine and their predicted penetration across the blood-brain barrier. Molecules 24, 1340 (2019). https://doi.org/10.3390/molecules24071340
2018
ŠAFRATOVÁ, M., HOŠŤÁLKOVÁ, A., HULCOVÁ, D., BREITEROVÁ, K., HRABCOVÁ, V., MACHADO, M., FONTINHA, D., PRUDENCIO, M., KUNEŠ, J., CHLEBEK, J., JUN, D., HRABINOVÁ, M., NOVÁKOVÁ, L., HAVELEK, R., SEIFERTOVÁ, M., OPLETAL, L., CAHLÍKOVÁ, L: Alkaloids from Narcissus poeticus cv. Pink Parasol of various structural types and their biological activity. Arch. Pharm. Res. 41, 208-218 (2018). https://doi.org/10.1007/s12272-017-1000-4
HABARTOVÁ, K., HAVELEK, R., SEIFERTOVÁ, M., KRÁLOVEC, K., CAHLÍKOVÁ, L., CHLEBEK, J., ČERMÁKOVÁ, E., MAZÁNKOVÁ, N., MAŘÍKOVÁ, J., KUNEŠ, J., NOVÁKOVÁ, L., ŘEZÁČOVÁ, M.: Scoulerine affects microtubule structure, inhibits proliferation, arrests cell cycle and thus culminates in the apoptotic death of cancer cells. Sci. Reports 8, 4829 (2018). https://doi.org/10.1038/s41598-018-22862-0
2017
SIATKA, T., CHLEBEK, J., HOŠŤÁLKOVÁ, A.: Copper(II) sulfate stimulates scopoletin production in cell suspension of Angelica archangelica. Nat. Prod. Commun. 12, 1779-1780 (2017). https://doi.org/10.1177/1934578X1701201133
SIATKA, T., ADAMCOVÁ, M., OPLETAL, L., CAHLÍKOVÁ, L., JUN, D., HRABINOVÁ, M., KUNEŠ, J., CHLEBEK, J.: Cholinesterase and prolyl oligopeptidase Inhibitory activities of alkaloids from Argemone platyceras (Papaveraceae). Molecules 22, 1181 (2017). https://doi.org/10.3390/molecules22071181
2016
CHLEBEK, J., DOSKOČIL, I., HULCOVÁ, D., BREITEROVÁ, K., ŠAFRATOVÁ, M., HAVELEK, R., HABARTOVÁ, K., HOŠŤÁLKOVÁ, A., VOLŠTÁTOVÁ, T., CAHLÍKOVÁ, L.: Cytotoxicity of naturally occurring isoquinoline alkaloids of different structural types. Nat. Prod. Commun. 11, 753-756 (2016). https://doi.org/10.1177/1934578X1601100614
CHLEBEK, J., NOVÁK, Z., KASSEMOVÁ, D., ŠAFRATOVÁ, M., KOSTELNÍK, J., MALÝ, L., LOČÁREK, M., OPLETAL, L., HOŠŤÁLKOVÁ, A., HRABINOVÁ, M., KUNEŠ, J., NOVOTNÁ, P., URBANOVÁ, M., NOVÁKOVÁ, L., MACÁKOVÁ, K., HULCOVÁ, D., SOLICH, P., MARTÍN, C.P., JUN, D., CAHLÍKOVÁ, L..: Isoquinoline alkaloids from Fumaria officinalis L. and their biological activities related to Alzheimer’s disease. Chem. Biodivers. 13, 91-99 (2016). https://doi.org/10.1002/cbdv.201500033
CHLEBEK, J., DE SIMONE, A., HOŠŤÁLKOVÁ, A., OPLETAL, L., PERÉZ, C., PERÉZ, D.I., HAVLÍKOVÁ, L., CAHLÍKOVÁ, L., ANDRISANO, V.: Application of BACE1 immobilized enzyme reactor for the characterization of multifunctional alkaloids from Corydalis cava (Fumariaceae) as Alzheimer’s disease targets. Fitoterapia 109, 241-247 (2016). https://doi.org/10.1016/j.fitote.2016.01.008
VANĚČKOVÁ, N., HOŠŤÁLKOVÁ, A., ŠAFRATOVÁ, M., KUNEŠ, J., HULCOVÁ, D., HRABINOVÁ, H., DOSKOČIL, I., ŠTĚPÁNKOVÁ, Š., OPLETAL, L., NOVÁKOVÁ, L., JUN, D., CHLEBEK, J., CAHLÍKOVÁ, L.: Isolation of Amaryllidaceae alkaloids from Nerine bowdenii W. Watson (Amaryllidaceae) and their biological activity. RSC Advances 6, 80114-80120 (2016). https://doi.org/10.1039/C6RA20205E
2015
ZEMANOVA, L., HOFMAN, J., NOVOTNA, E., MUSILEK, K., LUNDOVA, T., HAVRANKOVA, J., HOSTALKOVA, A., CHLEBEK, J., CAHLIKOVA, L., WSOL, V.: Flavones inhibit the activity of AKR1B10, a promising therapeutic target for cancer treatment. J. Nat. Prod. 78, 2666-2674 (2015). https://doi.org/10.1021/acs.jnatprod.5b00616
DOSKOČIL, I., HOŠŤÁLKOVÁ, A., ŠAFRATOVÁ, M., BENEŠOVÁ, N., HAVLÍK, J., HAVELEK, R., KUNEŠ, J., KRÁLOVEC, K., CHLEBEK, J., CAHLÍKOVÁ, L.: Cytotoxic activities of Amaryllidaceae alkaloids against gastrointestinal cancer cells. Phytochem. Lett. 13, 394-398 (2015). https://doi.org/10.1016/j.phytol.2015.08.004
CAHLÍKOVÁ, L., PÉREZ, D.I., ŠTĚPÁNKOVÁ, Š, CHLEBEK, J., ŠAFRATOVÁ, M., HOŠŤÁLKOVÁ, A., OPLETAL, L.: In vitro inhibitory effects of 8-O-demethylmaritidine and undulatine on acetylcholinesterase, and their predicted penetration across the blood-brain barrier. J. Nat. Prod. 78, 1189-1192 (2015). https://doi.org/10.1021/acs.jnatprod.5b00191
HOŠŤÁLKOVÁ, A., OPLETAL, L., KUNEŠ, J., NOVÁK, Z., HRABINOVÁ, M., CHLEBEK, J., CAHLÍKOVÁ, L.: Alkaloids from Peumus boldus Mol. and their acetylcholinesterase, butyrylcholinesterase and prolyl oligopeptidase inhibition activity. Nat.. Prod. Commun. 10, 577-580 (2015). https://doi.org/10.1177/1934578X1501000410
CAHLÍKOVÁ, L., HULOVÁ, L., HRABINOVÁ, M., CHLEBEK, J., HOŠŤÁLKOVÁ, A., ADAMCOVÁ, M., ŠAFRATOVÁ, M., JUN, D., OPLETAL, L., LOČÁREK, M., MACÁKOVÁ, K.: Isoquinoline alkaloids as potential prolyl oligopeptidase inhibitors. Fitoterapia 103, 192-196 (2015). https://doi.org/10.1016/j.fitote.2015.04.004
2014
OPLETAL, L., LOČÁREK, M., FRAŇKOVÁ, A., CHLEBEK, J., ŠMÍD, J., HOŠŤÁLKOVÁ, A., ŠAFRATOVÁ, M., HULCOVÁ, D., KLOUČEK P., ROZKOT, M., CAHLÍKOVÁ, L.: Antimicrobial activity of extracts and isoquinoline alkaloids of selected Papaveraceae plants. Nat. Prod. Commun. 9, 1709-1712 (2014). https://doi.org/10.1177/1934578X1400901211
ŠKARYDOVÁ, L., HOFMAN, J., CHLEBEK, J., HAVRÁNKOVÁ, J., KOŠANOVÁ, K., SKARKA, A., HOŠŤÁLKOVÁ, A., PLUCHA, T., CAHLÍKOVÁ, L., WSÓL, V.: Isoquinoline alkaloids as a novel type of AKR1C3 inhibitors. J. Steroid Biochem. Mol. Biol. 143, 250-258 (2014). https://doi.org/10.1016/j.jsbmb.2014.04.005
SCHRAMM, A., JÄHNE, E., BABURIN, I., HERING, S., CAHLÍKOVÁ, L., CHLEBEK, J., HAMBURGER, M.: Natural products as potential human ether-a-go-go-related gene channel inhibitors – screening of plant-derived alkaloids . Planta Med. 80, 740-746 (2014). https://doi.org/10.1055/s-0034-1368590
2013
CAHLÍKOVÁ, L., HRABINOVÁ, M., KULHÁNKOVÁ, A., BENEŠOVÁ, N., CHLEBEK, J., JUN, D., NOVÁK, Z., KUČA, K., MACÁKOVÁ, K., OPLETAL, L. Alkaloids from Chlidanthus fragrans Herb. and their acetylcholinesterase, butyrylcholinesterase and prolyl oligopeptidase inhibition activity. Nat. Prod. Commun. 8, 1541-1544 (2013). https://doi.org/10.1177/1934578X1300801110
CAHLÍKOVÁ, L., LOČÁREK, M., BENEŠOVÁ, N., KUČERA, R., CHLEBEK, J., NOVÁK, Z., OPLETAL, L.: Isolation and cholinesterase inhibitory activity of Narcissus extracts and Amaryllidaceae alkaloid. Nat. Prod. Commun. 8, 781-785 (2013). https://doi.org/10.1177/1934578X1300800625
2012
NOVÁK, Z., CHLEBEK, J., OPLETAL, L., JIROŠ, P., MACÁKOVÁ, K., KUNEŠ, J., CAHLÍKOVÁ, L.: Corylucinine, a new Alkaloid from Corydalis cava (Fumariaceae), and its cholinesterase activity. Nat. Prod. Commun. 7, 859-860 (2012). https://doi.org/10.1177/1934578X1200700712
2011
CHLEBEK, J., MACÁKOVÁ, K., CAHLÍKOVÁ, L., KURFÜRST, M., KUNEŠ, J., OPLETAL, L.: Acetylcholinesterase and butyrylcholinesterase inhibitory compounds from Corydalis cava Schweigger. & Korte (Fumariaceae). Nat. Prod. Commun. 6, 607-610 (2011). https://doi.org/10.1177/1934578X1100600507
2010
CAHLÍKOVÁ, L., MACÁKOVÁ, K., KUNEŠ, J., KURFÜRST, M., OPLETAL, L., CVAČKA, J., CHLEBEK, J., BLUNDEN, G.: Acetylcholinesterase and butyrylcholinesterase inhibitory compounds from Eschscholzia californica (Papaveraceae). Nat. Prod. Commun. 5, 1035-1038 (2010). https://doi.org/10.1177/1934578X1000500710).
Reviews
2015
HOŠŤÁLKOVÁ, A., SIATKA, T., CHLEBEK, J., OPLETAL, L., DRAŠAR, P., CAHLÍKOVÁ, L.: Boldinové alkaloidy a perspektivity jejich využití. Chem. listy 109, 846-855 (2015). http://chemicke-listy.cz/docs/full/2015_11_846-855.pdf
2013
OPLETAL, L., CHLEBEK, J., MACÁKOVÁ, K., BENEŠOVÁ, N., HOŠŤÁLKOVÁ, A., CAHLÍKOVÁ, L.: Antioxidanty a degenerativní onemocnění. Prakt. Lékáren. 9, 135-138 (2013). https://www.praktickelekarenstvi.cz/pdfs/lek/2013/03/11.pdf
2012
KOLEČKÁŘ, V., ŘEHÁKOVÁ, Z, BROJEROVÁ, E., KUČA, K., JUN, D., MACÁKOVÁ, K., OPLETAL, L., DRAŠAR, P., JAHODÁŘ, L., CHLEBEK, J., CAHLÍKOVÁ, L.: Proanthocyanidiny a jejich antioxidační aktivita. Chem. listy 106, 113-121 (2012). http://www.chemicke-listy.cz/ojs3/index.php/chemicke-listy/article/view/997/997
2011
CAHLÍKOVÁ, L., MACÁKOVÁ, K., CHLEBEK, J., HOŠŤÁLKOVÁ, A., KULHÁNKOVÁ, A., OPLETAL, L.: Ecdysterone and its activity on some degenerative diseases. Nat. Prod. Commun. 6, 707-718 (2011). https://doi.org/10.1177/1934578X1100600527