ADINACO Research Group

The research group (Alzheimer’s DIsease and NAtural COmpounds) deals with a wider study of substances isolated from plants and fungi of the Eumycota division from the point of view of their effects on patophysiological processes influencing the development of the Alzheimer’s disease. The group carries out isolation and identification of substances, determination of biological activity on a wide group of enzymes in CNS. In cooperation with other workplaces the group prepares partial derivatives of substances, and deals also with the study of the possibility of using substances in Alzheimer disease prevention in the form of food additives.


Mgr. Marcela Šafratová defended her dissertation thesis "Study of  inhibition (toxicity) activity of alkaloids from selected plant species of Amaryllidaceae family on human enzyme systems (in vitro study) III" in the field "Pharmacognosy and Toxicology of Natural Compounds" on 21st December 2016 and she received the degree "Ph.D.". Congratulations!!! Yesterday her daughter Sara was born!!! Best wishes Sara on your life journey!!!

Mgr. Daniela Hulcová, our Ph.D. student, has traveled to her first research fellowship to Andrisano’s group in Rimini (Italy) for six months. Daniela is becoming an expert in in vitro biological tests (BACE1 and GSK3 inhibition).


Current members of the group (from the left side): crouching people - Dr. Anna Hošťálková, Dr. Tomáš Siatka, a laboratory technician Petra Němcová, Dr. Jakub Chlebek, Mgr. Jan Němeček (he left our group last year on September), Ing. Miroslav Ločárek; standing people - Prof. Dr. Lubomír Opletal. Mgr. Marcela Šafratová, Dr. Kateřina Macáková, Assoc. Prof. Lucie Cahlíková (the head of the group), and Mgr. Danieal Hulcová.

Selected publications (2009 – 2017)


SIATKA, T., ADAMCOVÁ, M., OPLETAL, L., CAHLÍKOVÁ, L.,  JUN, D., HRABINOVÁ, M., KUNEŠ, J., CHLEBEK, J.: Cholinesterase and prolyl oligopeptidase Inhibitory activities of alkaloids from Argemone platyceras (Papaveraceae). Molecules 22, 1181 (2017).

HULCOVÁ, D., BREITEROVÁ, K., ZEMANOVÁ, L., ŠAFRATOVÁ, M., VANĚČKOVÁ, N., HOŠŤÁLKOVÁ, A., WSÓL, V., CAHLÍKOVÁ, L.: Screening of AKR1C3 inhibitory potency of naturally occuring Amaryllidaceae alkaloids of different structural types. Nat. Prod. Commun. 12, 245-246 (2017).

OPLETAL, L., CHOCHOLOUŠOVÁ-HAVLÍKOVÁ, L., SIATKA, T., CAHLÍKOVÁ, L., LOČÁREK, M., BADRELDIN, H. A., MANOJ, P., RAMKUMAR, A., Al SULEIMANI, Y. M., AL ZA‘ABI, M., KARACA, T., NEMMAR, A.: Preparation and validated analysis of anthocyanin concentrate from the calyces of Hibiscus sabdariffa. Nat. Prod. Commun. 12(1), 43-45 (2017).

ALI, B. H., CAHLÍKOVÁ, L., OPLETAL, L., KARACA, T., MANOJ, P., RAMKUMAR, A., AL SULEIMANI Y. M., AL ZA'ABI, M., NEMMAR, A., CHOCHOLOUŠOVÁ-HAVLÍKOVÁ, L., LOČÁREK, M., SIATKA, T., BLUNDEN, G.: Effect of aqueous extract and anthocyanins of calces of Hibiscus sabdariffa (Malvaceae) in rats with adenine-induced chronic kidney disease. J. Pharm. Pharmacol., DOI 101111/jphp.12748 (2017).


HABARTOVÁ, K., CAHLÍKOVÁ, L., ŘEZÁČOVÁ M., HAVELEK, R.: The biological activity of alkaloids from the Amaryllidaceae: from cholinesterases inhibition to anticancer activity. Nat. Prod. Commun., accepted manuscript.

VANĚČKOVÁ, N., HOŠŤÁLKOVÁ, A., ŠAFRATOVÁ, M., KUNEŠ, J., HULCOVÁ, D., HRABINOVÁ, H., DOSKOČIL, I., ŠTĚPÁNKOVÁ, Š., OPLETAL, L., NOVÁKOVÁ, L., JUN, D., CHLEBEK, J., CAHLÍKOVÁ, L.: Alkaloids from Nerine bowdenii W. Watson (Amaryllidaceae) and their biological activity. RSC Advances 6, 80114-80120 (2016).

DOSKOČIL, I., HAVLÍK, J., VERLOTTA, R., TAUCHEN, J., VESELA, L., MACAKOVA, K., OPLETAL, L., KOKOSKA, L., RADA, V.: In vitro immunomodulatory activity, cytotoxicity and Chemistry of some central European polypores.  Pharm. Biol. 54, 2369-2376 (2016).

HAVELEK, R., SEIFERTOVÁ, M., KRÁLOVEC, K., KROCOVÁ, E., TEJKALOVÁ, V., NOVOTNÝ, I., CAHLÍKOVÁ, L., ŠAFRATOVÁ, M., OPLETAL, L., BÍLKOVÁ, Z., VÁVROVÁ, J., ŘEZÁČOVÁ, M.: Homochelidonine, the dimethoxy analogue of chelidonine isolated from Chelidonium majus L. (Papaveraceae), decreases proliferation via G1 and G2/M cell cycle arrest and induces cell death through apoptosis. Phytomedicine  23, 253-266 (2016).

HROCH, M., HAVELEK, R., CAHLÍKOVÁ, L., HOŠŤÁLKOVÁ, A., HULCOVÁ, D., CERMANOVÁ, J., MIČUDA, S.: LC-MS/MS method for determination of haemanthamine in rat plasma, bile and urine: application to a pilot pharmacokinetic study. Biomed. Chrom. 30, 1083-1091 (2016).

CHLEBEK, J., DOSKOČIL, I., HULCOVÁ, D., BREITEROVÁ, K., ŠAFRATOVÁ, M., HAVELEK, R., HABARTOVÁ, K., HOŠŤÁLKOVÁ, A., VOLŠTÁTOVÁ, T., CAHLÍKOVÁ, L.: Cytotoxicity of naturally occurring isoquinoline alkaloids of different structural types. Nat. Prod. Commun11, 753-756 (2016).

CHLEBEK, J., NOVÁK, Z., KASSEMOVÁ, D., ŠAFRATOVÁ, M., KOSTELNÍK, J., MALÝ, L., LOČÁREK, M., OPLETAL, L., HOŠŤÁLKOVÁ, A., HRABINOVÁ, M., KUNEŠ, J., NOVOTNÁ, P., URBANOVÁ, M., NOVÁKOVÁ, L., MACÁKOVÁ, K., HULCOVÁ, D., SOLICH, P., MARTÍN, C.P., JUN, D., CAHLÍKOVÁ, L..: Isoquinoline alkaloids from Fumaria officinalis L. and their biological activities related to Alzheimer’s disease. Chem. Biodivers. 13, 91-99 (2016).

CHLEBEK, J., DE SIMONE, A., HOŠŤÁLKOVÁ, A., OPLETAL, L., PERÉZ, C., PERÉZ, D.I., HAVLÍKOVÁ, L., CAHLÍKOVÁ, L., ANDRISANO, V.: Application of BACE1 immobilized enzyme reactor for the characterization of multifunctional alkaloids from Corydalis cava (Fumariaceae) as Alzheimer’s disease targets. Fitoterapia 109, 241-247 (2016).


DOSKOČIL, I., HOŠŤÁLKOVÁ, A., ŠAFRATOVÁ, M., BENEŠOVÁ, N., HAVLÍK, J., HAVELEK, R., KUNEŠ, J., KRÁLOVEC, K., CHLEBEK, J., CAHLÍKOVÁ, L.: Cytotoxic activities of Amaryllidaceae alkaloids against gastrointestinal cancer cells. Phytochem. Lett. 13, 394-398 (2015).

NOVÁK, Z., HOŠŤÁLKOVÁ, A., OPLETAL, L., NOVÁKOVÁ, L., HRABINOVÁ, M., KUNEŠ, J., CAHLÍKOVÁ, L.: (+)-Chenabinol (revised NMR data) and two new alkaloids from Berberis vulgaris L. and their biological activity. Nat. Prod. Commun. 10, 1695-1697 (2015). 

CAHLÍKOVÁ, L., PÉREZ, D.I., ŠTĚPÁNKOVÁ, Š, CHLEBEK, J., ŠAFRATOVÁ, M., HOŠŤÁLKOVÁ, A., OPLETAL, L.: In vitro inhibitory effects of 8-O-Demethylmaritidine and undulatine on acetylcholinesterase, and their predicted penetration across the blood-brain barrier. J. Nat. Prod. 78, 1189-1192 (2015).

HOŠŤÁLKOVÁ, A., OPLETAL, L., KUNEŠ, J., NOVÁK, Z., HRABINOVÁ, M., CHLEBEK, J., CAHLÍKOVÁ, L.: Alkaloids from Peumus boldus Mol. and their acetylcholinesterase, butyrylcholinesterase and prolyl oligopeptidase inhibition activity. Nat.. Prod. Commun. 10, 577-580 (2015).

CAHLÍKOVÁ, L., HULOVÁ, L., HRABINOVÁ, M., CHLEBEK, J., HOŠŤÁLKOVÁ, A., ADAMCOVÁ, M., ŠAFRATOVÁ, M., JUN, D., OPLETAL, L., LOČÁREK, M., MACÁKOVÁ, K.: Isoquinoline alkaloids as potential prolyl oligopeptidase inhibitors. Fitoterapia 103, 192-196 (2015).

CAHLÍKOVÁ, L., ALI B.H., HAVLÍKOVÁ L., LOČÁREK, M., SIATKA, T., OPLETAL, L., BLUNDEN, G.: Anthocyanins of Hibiscus sabdiffera Calyces from Sudan. Nat. Prod. Commun.  10, 77-79 (2015).


MACÁKOVÁ, K., KOLEČKÁŘ, V., CAHLÍKOVÁ, L., CHLEBEK, J., HOŠŤÁLKOVÁ, A., KUČA, K., JUN, D., OPLETAL, L.: Tannins and their influence on health. Recent Advances in Medicinal Chemistry. Bentham Science Publishers 1, 159-208 (2014).

CAHLÍKOVÁ, L., MACÁKOVÁ, K., BENEŠOVÁ, N., CHLEBEK, J., HOŠŤÁLKOVÁ, A., OPLETAL, L.: Natural Componuds (Small Molecules) as Potential and Real Drugs of Alzheimer´s Disease: A Critical Review, in: Studies in Natural Products Chemistry (ATTA-ur-RAHMAN, ed.), Chapter 6, Elsevier: Amsterdam 2014, pp. 153-194, ISBN: 978-0-44

OPLETAL, L., LOČÁREK, M., FRAŇKOVÁ, A., CHLEBEK, J., ŠMÍD, J., HOŠŤÁLKOVÁ, A., ŠAFRATOVÁ, M., HULCOVÁ, D., KLOUČEK P., ROZKOT, M., CAHLÍKOVÁ, L.: Antimicrobial activity of extracts and isoquinoline alkaloids of selected Papaveraceae plants. Nat. Prod. Commun. 9, 1709-1712 (2014).

ŠKARYDOVÁ, L., HOFMAN, J., CHLEBEK, J., HAVRÁNKOVÁ, J., KOŠANOVÁ, K., SKARKA, A., HOŠŤÁLKOVÁ, A., PLUCHA, T., CAHLÍKOVÁ, L., WSÓL, V.: Isoquinoline alkaloids as a novel type of AKR1C3 inhibitors. J. Steroid Biochem. Mol. Biol. 143, 250-258 (2014).

SCHRAMM, A., JÄHNE, E., BABURIN, I., HERING, S., CAHLÍKOVÁ, L., CHLEBEK, J., HAMBURGER, M.: Natural products as potential hERG channel inhibitors –  screening of plant-derived alkaloids. Planta Med. 80, 740-746 (2014).

ŠAFRATOVÁ, M., NOVÁK, Z., KULHÁNKOVÁ, A., KUNEŠ, J., HRABINOVÁ, M., JUN, D., MACÁKOVÁ, K., OPLETAL, L., CAHLÍKOVÁ, L.: Revised NMR data for 9-O-demethylgalanthine: an alkaloid from Zephyranthes robusta (Amaryllidaceae) and its biological activity. Nat. Prod. Commun. 9, 787-788 (2014).

HAVLASOVÁ, J., ŠAFRATOVÁ, M., SIATKA T., ŠTĚPÁNOVÁ, Š., LOČÁREK, M., OPLETAL, L., HRABINOVÁ, M., JUN, D., BENEŠOVÁ, N., NOVÁK, Z., KUNEŠ, J., CAHLÍKOVÁ, L.: Chemical composition of bioactive alkaloid extracts from some Narcissus species and varieties and their biological activity. Nat. Prod. Commun. 9, 1151-1155 (2014).

HAVELEK, R., SEIFERTOVÁ, M., KRÁLOVEC, K., BRŮČKOVÁ, L., CAHLÍKOVÁ, L., DALECKÁ, M., VÁVROVÁ, J., ŘEZÁČOVÁ, M., OPLETAL, L., BÍLKOVÁ, Z.: The effect of Amaryllidaceae alkaloids haemanthamine and haemanthidine on cell cycle progression and apoptosis in p53-negative human leukemic Jurkat cell. Phytomedicine 21, 479-490 (2014).

ŘEHÁKOVÁ, Z., KOLEČKÁŘ, V., JAHODÁŘ, L., OPLETAL, L., MACÁKOVÁ, K., CAHLÍKOVÁ, L., JUN, D., KUČA, K.: Evaluation of the antioxidants activity of several naturally occuring coumarins and their synthesized analouges by „ferric reducing antioxidant power“ assay. J. Enzyme Inhib. Med. Chem. 29, 49-54 (2014).


CAHLÍKOVÁ, L., HRABINOVÁ, M., KULHÁNKOVÁ, A., BENEŠOVÁ, N., CHLEBEK, J., JUN, D., NOVÁK, Z., KUČA, K., MACÁKOVÁ, K., OPLETAL, L. Alkaloids from Chlidanthus fragrans Herb. and their acetylcholinesterase, butyrylcholinesterase and prolyl oligopeptidase inhibition activity. Nat. Prod. Commun. 8, 1541-1544 (2013).

CAHLÍKOVÁ, L., LOČÁREK, M., BENEŠOVÁ, N., KUČERA, R., CHLEBEK, J., NOVÁK, Z., OPLETAL, L.: Isolation and cholinesterase inhibitory activity of Narcissus extracts and Amaryllidaceae alkaloid. Nat. Prod. Commun. 8, 781-785 (2013).

HOŠŤÁLKOVÁ, A., NOVÁK, Z., POUR, M., JIROŠOVÁ, A., OPLETAL, L., KUNEŠ, J., CAHLÍKOVÁ, L.: Berbanine: a new isoquinoline alkaloid from Berberis vulgaris (Berberidaceae). Nat. Prod. Commun. 8, 441-442 (2013).

KULHÁNKOVÁ, A., CAHLÍKOVÁ, L., NOVÁK, Z., MACÁKOVÁ, K., KUNEŠ, J., OPLETAL, L.: Alkaloids from Zephyranthes robusta Baker and their acetylcholinesterase and butyrylcholinesterase inhibition activity. Chem. Biodivers. 10, 1120-1127 (2013).

DALECKÁ, M., HAVELEK, R., KRALOVEC, K., BRUČKOVÁ, L., CAHLÍKOVÁ, L.: Amaryllidaceae family alkaloids as potential drugs for cancer treatment. Chem. listy 107, 701-708 (2013).


NOVÁK, Z., CHLEBEK, J., OPLETAL, L., JIROŠ, P., MACÁKOVÁ, K., KUNEŠ, J., CAHLÍKOVÁ, L.: Corylucinine, a new Alkaloid from Corydalis cava (Fumariaceae), and its Cholinesterase Activity. Nat. Prod. Commun. 7, 859-860 (2012).

CAHLÍKOVÁ, L., KUČERA, R., HOŠŤÁLKOVÁ, A., KLIMEŠ, J., OPLETAL, L.: Identification of pavinane alkaloids in the genera Argemone and Eschscholzia by GC-MS. Nat. Prod. Commun. 7, 1279-1281 (2012).

CAHLÍKOVÁ, L., BENEŠOVÁ, N., MACÁKOVÁ, K., KUČERA, R., HRSTKA, V., KLIMEŠ, J., JAHODÁŘ, L., OPLETAL, L.: Alkaloids of some Amaryllidaceae species and their cholinesterase activity. Nat. Prod. Commun. 7, 571-574 (2012).


CAHLÍKOVÁ, L., MACÁKOVÁ, K., ZAVADIL, S., JIROŠ, P., OPLETAL, L., URBANOVÁ, K., JAHODÁŘ, L.: Analysis of Amaryllidaceae alkaloids from Chlidanthus fragrans and their cholinesterase activity. Nat. Prod. Commun. 6, 603-606 (2011).

CAHLÍKOVÁ, L., VALTEROVÁ, I., MACÁKOVÁ, K., OPLETAL, L.: Analysis of Amaryllidaceae alkaloids from Zephyranthes grandiflora Lindl. by GC/MS and their cholinesterase activity. Rev. Brasil. Farmacogn. 21, 575-580 (2011).

CAHLÍKOVÁ, L., ZAVADIL, S., MACÁKOVÁ, K., VALTEROVÁ, I., KULHÁNKOVÁ, A., HOŠŤÁLKOVÁ, A., KUNEŠ, J., OPLETAL, L.: Isolation and cholinesterase activity of Amaryllidaceae alkaloids from Nerine bowdenii. Nat. Prod. Commun. 6, 1827-1830 (2011).

CHLEBEK, J., MACÁKOVÁ, K., CAHLÍKOVÁ, L., KURFÜRST, M., KUNEŠ, J, OPLETAL, L.: Acetylcholinesterase and butyrylcholinesterase inhibitory compounds from Corydalis cava Schweigger. & Korte (Fumariaceae). Nat. Prod. Commun. 6, 607-610 (2011).


CAHLÍKOVÁ, L., KULHÁNKOVÁ, A., URBANOVÁ, K., VALTEROVÁ, I., MACÁKOVÁ, K., KUNEŠ, J.: Analysis of Amaryllidaceae Alkaloids from Zephyranthes robusta by GC-MS and Their Cholinesterase Activity. Nat. Prod. Commun. 5, 1201-1204 (2010).

CAHLÍKOVÁ, L., MACÁKOVÁ, K., KUNEŠ, J., KURFÜRST, M., OPLETAL, L., CVAČKA, J., CHLEBEK, J., BLUNDEN, G.: Acetylcholinesterase and butyrylcholinesterase inhibitory compounds from Eschscholzia californica (Papaveraceae). Nat. Prod. Commun. 5, 1035-1038 (2010).

CAHLÍKOVÁ, L., OPLETAL, L., KURFÜRST, M., MACÁKOVÁ, K., KULHÁNKOVÁ, A., HOŠŤÁLKOVÁ, A.: Acetylcholinesterase and butyrylcholinesterase inhibitory compounds from Chelidonium majus (Papaveraceae). Nat. Prod. Commun. 5, 1751-1754 (2010).

MACÁKOVÁ, K., OPLETAL, L., POLÁŠEK, M., SAMKOVÁ, V.: Free-radical scavenging activity of some european Polyporales. Nat. Prod. Commun. 5, 923-926 (2010).

OPLETAL, L.: Přírodní látky a jejich biologická aktivita. Sv. 1. Nutraceutika. Primární metabolity a látky obsažené ve strukturovaných biologických systémech (Natural Compounds and Their Biological Activity, Vol. 1. Nutraceutics. Primary Metabolites and Compounds Contained in Structured Biological Systems). Karolinum, Praha 2010, ISBN 978-80-246-1884-5, 378 pp.


MACÁKOVÁ, K., OPLETAL, L., POLÁŠEK, M., SAMKOVÁ, V., JAHODÁŘ, L.: Free-radical scavenging activity of some European Boletales. Nat. Prod. Commun. 4, 261-264 (2009).

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