Assoc. Prof. PharmDr. Jakub Chlebek, Ph.D.

Research focus

  • isolation of secondary metabolites from plant families Fumariaceae, Papaveraceae and Amaryllidaceae (isolation, analysis, structural identification)
  • in vitro biological activities of isolated compounds (AChE, BuChE, POP, GSK-3β and BACE1 inhibition activity and antioxidative activity)

Education

  • 2002-2007 Charles University in Prague, Faculty of Pharmacy, (Master degree), Thesis: Pharmacognostic study of Alnus spp.
  • 2007-2012 Charles University in Prague, Faculty of Pharmacy in Hradec Králové, Department of Pharmaceutical Botany and Ecology, (Doctoral degree), Thesis: Study of biological activity of alkaloids isolated from Corydalis cava (Fumariaceae)

Current position

  • associate professor at Charles University, Faculty of Pharmacy in Hradec Králové, Department of Pharmaceutical Botany

Teaching activities

  • Pharmaceutical botany (practicals)
  • Toxicity of natural products (lectures)
  • Phytochemical methods (practicals)
  • Forensic and clinical analysis of toxic compounds (lectures)

Research fellowship

  • 2011 University of Bologna, Italy, Department of Pharmaceutical Sciences – supervisor prof. Vincenza Andrisano (5 months)
  • 2014 Medicinal Chemistry Institute-CSIC in Madrid, Spain – supervisor prof. Ana Martinez (2 months)
  • 2018 University of Antwerp, Department of Pharmaceutical Sciences – supervisor Dr. Emmy Tuenter (1 month)

Professional membership

  • since 2009 – Czech Chemical Society
  • since 2007-2013 – Czech Apothecary Association

Grant recieved

  • Chlebek, J., Cahlíková, L., Macáková, K., Opletal, L.: Plant secondary metabolites from subclasses Magnoliidae, Ranunculidae, Cornidae and Lamiidae influencing in vitro activity of human cholinesterases as a contribution to study of Alzheimerʼs disease: bio-guided isolation strategy. Grant Agency of Charles University, No. 122309/2009/B-BIO (2009–2011).

Publications

Monographs (and chapters)

2014

MACÁKOVÁ, K., KOLEČKÁŘ, V., CAHLÍKOVÁ, L., CHLEBEK, J., HOŠŤÁLKOVÁ, A., KUČA, K., JUN, D., OPLETAL, L.: Tannins and their influence on health. Recent Advances in Medicinal Chemistry. Bentham Science Publishers 1, 159-208 (2014).

CAHLÍKOVÁ, L., MACÁKOVÁ, K., BENEŠOVÁ, N., CHLEBEK, J., HOŠŤÁLKOVÁ, A., OPLETAL, L.: Natural compounds (small molecules) as potential and real drugs of Alzehimer’s disease: A critical review, in: Studies in natural products chemistry (ATTA-ur-RAHMAN, ed.), Chapter 6, Elsevier: Amsterdam 2014, pp. 153-194, ISBN: 978-0-444-63281-4.

2010

OPLETAL, L., ROZKOT, M., CHLEBEK, J., ČEŘOVSKÝ, J.: Látky přírodního původu potenciálně ovlivňující reprodukci prasat, in: OPLETAL, L., SKŘIVANOVÁ, V. (eds): Přírodní látky a jejich biologická aktivita. Sv. 2: Využití látek pro ovlivnění fyziologických procesů hospodářských zvířat, s. 517–582, Karolinum, Praha 2010, ISBN 978-80-246-1801-2.

Original papers

2021

KOHELOVÁ, E., MAŘÍKOVÁ, J., KORÁBEČNÝ, J., HULCOVÁ, D., KUČERA, T., JUN, D., CHLEBEK, J., JENČO, J., ŠAFRATOVÁ, M., HRABINOVÁ, M., RITOMSKÁ, A., MALANÍK, M., PEŘINOVÁ, R., BREITEROVÁ, K., KUNEŠ, J., NOVÁKOVÁ, L., OPLETAL, L., CAHLÍKOVÁ, L.: Alkaloids of Zephyrantes citrina (Amaryllidaceae) and their implication to Alzheimerʼs disease: isolation, structural elucidation and biological activity. Bioorg. Chem. 107, 104567 (2021). https://doi.org/10.1016/j.bioorg.2020.104567

2019

CHLEBEK, J., KORÁBEČNÝ, J., DOLEŽAL, R., ŠTĚPÁNKOVÁ, Š., PÉREZ, D.I., HOŠŤÁLKOVÁ, A., OPLETAL, L., CAHLÍKOVÁ, L., MACÁKOVÁ, K., KUČERA, T., HRABINOVÁ, M., JUN, D. In vitro and in silico acetylcholinesterase inhibitory activity of thalictricavine and canadine and their predicted penetration across the blood-brain barrier. Molecules 24, 1340 (2019). https://doi.org/10.3390/molecules24071340

2018

ŠAFRATOVÁ, M., HOŠŤÁLKOVÁ, A., HULCOVÁ, D., BREITEROVÁ, K., HRABCOVÁ, V., MACHADO, M., FONTINHA, D., PRUDENCIO, M., KUNEŠ, J., CHLEBEK, J., JUN, D., HRABINOVÁ, M., NOVÁKOVÁ, L., HAVELEK, R., SEIFERTOVÁ, M., OPLETAL, L., CAHLÍKOVÁ, L: Alkaloids from Narcissus poeticus cv. Pink Parasol of various structural types and their biological activity. Arch. Pharm. Res. 41, 208-218 (2018).  https://doi.org/10.1007/s12272-017-1000-4

HABARTOVÁ, K., HAVELEK, R., SEIFERTOVÁ, M., KRÁLOVEC, K., CAHLÍKOVÁ, L., CHLEBEK, J., ČERMÁKOVÁ, E., MAZÁNKOVÁ, N., MAŘÍKOVÁ, J., KUNEŠ, J., NOVÁKOVÁ, L., ŘEZÁČOVÁ, M.: Scoulerine affects microtubule structure, inhibits proliferation, arrests cell cycle and thus culminates in the apoptotic death of cancer cells. Sci. Reports 8,  4829 (2018). https://doi.org/10.1038/s41598-018-22862-0

2017

SIATKA, T., CHLEBEK, J., HOŠŤÁLKOVÁ, A.: Copper(II) sulfate stimulates scopoletin production in cell suspension of Angelica archangelica. Nat. Prod. Commun. 12, 1779-1780 (2017). https://doi.org/10.1177/1934578X1701201133

SIATKA, T., ADAMCOVÁ, M., OPLETAL, L., CAHLÍKOVÁ, L.,  JUN, D., HRABINOVÁ, M., KUNEŠ, J., CHLEBEK, J.: Cholinesterase and prolyl oligopeptidase Inhibitory activities of alkaloids from Argemone platyceras (Papaveraceae). Molecules 22, 1181 (2017). https://doi.org/10.3390/molecules22071181

2016

CHLEBEK, J., DOSKOČIL, I., HULCOVÁ, D., BREITEROVÁ, K., ŠAFRATOVÁ, M., HAVELEK, R., HABARTOVÁ, K., HOŠŤÁLKOVÁ, A., VOLŠTÁTOVÁ, T., CAHLÍKOVÁ, L.: Cytotoxicity of naturally occurring isoquinoline alkaloids of  different structural types. Nat. Prod. Commun11, 753-756 (2016). https://doi.org/10.1177/1934578X1601100614

CHLEBEK, J., NOVÁK, Z., KASSEMOVÁ, D., ŠAFRATOVÁ, M., KOSTELNÍK, J., MALÝ, L., LOČÁREK, M., OPLETAL, L., HOŠŤÁLKOVÁ, A., HRABINOVÁ, M., KUNEŠ, J., NOVOTNÁ, P., URBANOVÁ, M., NOVÁKOVÁ, L., MACÁKOVÁ, K., HULCOVÁ, D., SOLICH, P., MARTÍN, C.P., JUN, D., CAHLÍKOVÁ, L..: Isoquinoline alkaloids from Fumaria officinalis L. and their biological activities related to Alzheimer’s disease. Chem. Biodivers. 13, 91-99 (2016). https://doi.org/10.1002/cbdv.201500033

CHLEBEK, J., DE SIMONE, A., HOŠŤÁLKOVÁ, A., OPLETAL, L., PERÉZ, C., PERÉZ, D.I., HAVLÍKOVÁ, L., CAHLÍKOVÁ, L., ANDRISANO, V.: Application of BACE1 immobilized enzyme reactor for the characterization of multifunctional alkaloids from Corydalis cava (Fumariaceae) as Alzheimer’s disease targets. Fitoterapia 109, 241-247 (2016). https://doi.org/10.1016/j.fitote.2016.01.008

VANĚČKOVÁ, N., HOŠŤÁLKOVÁ, A., ŠAFRATOVÁ, M., KUNEŠ, J., HULCOVÁ, D., HRABINOVÁ, H., DOSKOČIL, I., ŠTĚPÁNKOVÁ, Š., OPLETAL, L., NOVÁKOVÁ, L., JUN, D., CHLEBEK, J., CAHLÍKOVÁ, L.: Isolation of Amaryllidaceae alkaloids from Nerine bowdenii W. Watson (Amaryllidaceae) and their biological activity. RSC Advances 6, 80114-80120 (2016). https://doi.org/10.1039/C6RA20205E

2015

ZEMANOVA, L., HOFMAN, J., NOVOTNA, E., MUSILEK, K., LUNDOVA, T., HAVRANKOVA, J., HOSTALKOVA, A., CHLEBEK, J., CAHLIKOVA, L., WSOL, V.: Flavones inhibit the activity of AKR1B10, a promising therapeutic target for cancer treatment. J. Nat. Prod. 78, 2666-2674 (2015). https://doi.org/10.1021/acs.jnatprod.5b00616

DOSKOČIL, I., HOŠŤÁLKOVÁ, A., ŠAFRATOVÁ, M., BENEŠOVÁ, N., HAVLÍK, J., HAVELEK, R., KUNEŠ, J., KRÁLOVEC, K., CHLEBEK, J., CAHLÍKOVÁ, L.: Cytotoxic activities of Amaryllidaceae alkaloids against gastrointestinal cancer cells. Phytochem. Lett. 13, 394-398 (2015). https://doi.org/10.1016/j.phytol.2015.08.004

CAHLÍKOVÁ, L., PÉREZ, D.I., ŠTĚPÁNKOVÁ, Š, CHLEBEK, J., ŠAFRATOVÁ, M., HOŠŤÁLKOVÁ, A., OPLETAL, L.: In vitro inhibitory effects of 8-O-demethylmaritidine and undulatine on acetylcholinesterase, and their predicted penetration across the blood-brain barrier. J. Nat. Prod. 78, 1189-1192 (2015). https://doi.org/10.1021/acs.jnatprod.5b00191

HOŠŤÁLKOVÁ, A., OPLETAL, L., KUNEŠ, J., NOVÁK, Z., HRABINOVÁ, M., CHLEBEK, J., CAHLÍKOVÁ, L.: Alkaloids from Peumus boldus Mol. and their acetylcholinesterase, butyrylcholinesterase and prolyl oligopeptidase inhibition activity. Nat.. Prod. Commun. 10, 577-580 (2015). https://doi.org/10.1177/1934578X1501000410

CAHLÍKOVÁ, L., HULOVÁ, L., HRABINOVÁ, M., CHLEBEK, J., HOŠŤÁLKOVÁ, A., ADAMCOVÁ, M., ŠAFRATOVÁ, M., JUN, D., OPLETAL, L., LOČÁREK, M., MACÁKOVÁ, K.: Isoquinoline alkaloids as potential prolyl oligopeptidase inhibitors. Fitoterapia 103, 192-196 (2015). https://doi.org/10.1016/j.fitote.2015.04.004

2014

OPLETAL, L., LOČÁREK, M., FRAŇKOVÁ, A., CHLEBEK, J., ŠMÍD, J., HOŠŤÁLKOVÁ, A., ŠAFRATOVÁ, M., HULCOVÁ, D., KLOUČEK P., ROZKOT, M., CAHLÍKOVÁ, L.: Antimicrobial activity of extracts and isoquinoline alkaloids of selected Papaveraceae plants. Nat. Prod. Commun. 9, 1709-1712 (2014). https://doi.org/10.1177/1934578X1400901211

ŠKARYDOVÁ, L., HOFMAN, J., CHLEBEK, J., HAVRÁNKOVÁ, J., KOŠANOVÁ, K., SKARKA, A., HOŠŤÁLKOVÁ, A., PLUCHA, T., CAHLÍKOVÁ, L., WSÓL, V.: Isoquinoline alkaloids as a novel type of AKR1C3 inhibitors. J. Steroid Biochem. Mol. Biol. 143, 250-258 (2014). https://doi.org/10.1016/j.jsbmb.2014.04.005 

SCHRAMM, A., JÄHNE, E., BABURIN, I., HERING, S., CAHLÍKOVÁ, L., CHLEBEK, J., HAMBURGER, M.: Natural products as potential human ether-a-go-go-related gene channel inhibitors – screening of plant-derived alkaloids . Planta Med. 80, 740-746 (2014). https://doi.org/10.1055/s-0034-1368590

2013

CAHLÍKOVÁ, L., HRABINOVÁ, M., KULHÁNKOVÁ, A., BENEŠOVÁ, N., CHLEBEK, J., JUN, D., NOVÁK, Z., KUČA, K., MACÁKOVÁ, K., OPLETAL, L. Alkaloids from Chlidanthus fragrans Herb. and their acetylcholinesterase, butyrylcholinesterase and prolyl oligopeptidase inhibition activity. Nat. Prod. Commun. 8, 1541-1544 (2013). https://doi.org/10.1177/1934578X1300801110

CAHLÍKOVÁ, L., LOČÁREK, M., BENEŠOVÁ, N., KUČERA, R., CHLEBEK, J., NOVÁK, Z., OPLETAL, L.: Isolation and cholinesterase inhibitory activity of Narcissus extracts and Amaryllidaceae alkaloid. Nat. Prod. Commun. 8, 781-785 (2013). https://doi.org/10.1177/1934578X1300800625

2012

NOVÁK, Z., CHLEBEK, J., OPLETAL, L., JIROŠ, P., MACÁKOVÁ, K., KUNEŠ, J., CAHLÍKOVÁ, L.: Corylucinine, a new Alkaloid from Corydalis cava (Fumariaceae), and its cholinesterase activity. Nat. Prod. Commun. 7, 859-860 (2012). https://doi.org/10.1177/1934578X1200700712

2011

CHLEBEK, J., MACÁKOVÁ, K., CAHLÍKOVÁ, L., KURFÜRST, M., KUNEŠ, J., OPLETAL, L.: Acetylcholinesterase and butyrylcholinesterase inhibitory compounds from Corydalis cava Schweigger. & Korte (Fumariaceae). Nat. Prod. Commun. 6, 607-610 (2011).  https://doi.org/10.1177/1934578X1100600507

2010

CAHLÍKOVÁ, L., MACÁKOVÁ, K., KUNEŠ, J., KURFÜRST, M., OPLETAL, L., CVAČKA, J., CHLEBEK, J., BLUNDEN, G.: Acetylcholinesterase and butyrylcholinesterase inhibitory compounds from Eschscholzia californica (Papaveraceae). Nat. Prod. Commun. 5, 1035-1038 (2010). https://doi.org/10.1177/1934578X1000500710).

Reviews

2015

HOŠŤÁLKOVÁ, A., SIATKA, T., CHLEBEK, J., OPLETAL, L., DRAŠAR, P., CAHLÍKOVÁ, L.: Boldinové alkaloidy a perspektivity jejich využití. Chem. listy 109, 846-855 (2015). http://chemicke-listy.cz/docs/full/2015_11_846-855.pdf

2013

OPLETAL, L., CHLEBEK, J., MACÁKOVÁ, K., BENEŠOVÁ, N., HOŠŤÁLKOVÁ, A., CAHLÍKOVÁ, L.: Antioxidanty a degenerativní onemocnění. Prakt. Lékáren. 9, 135-138 (2013). https://www.praktickelekarenstvi.cz/pdfs/lek/2013/03/11.pdf

2012

KOLEČKÁŘ, V., ŘEHÁKOVÁ, Z, BROJEROVÁ, E., KUČA, K., JUN, D., MACÁKOVÁ, K., OPLETAL, L., DRAŠAR, P., JAHODÁŘ, L., CHLEBEK, J., CAHLÍKOVÁ, L.: Proanthocyanidiny a jejich antioxidační aktivita. Chem. listy 106, 113-121 (2012). http://www.chemicke-listy.cz/ojs3/index.php/chemicke-listy/article/view/997/997

2011

CAHLÍKOVÁ, L., MACÁKOVÁ, K., CHLEBEK, J., HOŠŤÁLKOVÁ, A., KULHÁNKOVÁ, A., OPLETAL, L.: Ecdysterone and its activity on some degenerative diseases. Nat. Prod. Commun. 6, 707-718 (2011). https://doi.org/10.1177/1934578X1100600527

© Charles University, Faculty of Pharmacy in Hradec Králové, Akademika Heyrovského 1203, 500 05 Hradec Králové, Czech Republic
Website information | Powered by Kentico