Assoc. Prof. PharmDr. Petr Chocholouš, Ph.D.


Born in 1980

Contact Information:
Faculty of Pharmacy, Charles University
Heyrovského 1203
CZ 50005 Hradec Králové

Tel.: +420 495 067 228
Fax: +420 495 067 164

ORCID: 0000-0002-6919-6449
ResearcherID: S-5566-2017
Scopus Author ID: 6507339778
Web of Science: ACM-4779-2022


Profile at a glance

Petr Chocholouš received his Ph.D. in Pharmaceutical Analysis from the Charles University, where he was Dalibor Šatínský and Petr Solich postgraduate student. He spent seven months at the University of Washington as a postdoctoral fellow, where he worked under the supervision of the father of Flow Analysis – prof. Jarda Růžička. His current position is Associate Professor at the Faculty of Pharmacy, Charles University.

His research interests have spanned a wide range of topics in Flow Analysis and related methods. His career started in 2002 with the born of Sequential Injection Chromatography (SIC), a chromatographic technique hyphenating commercial monolithic column with Sequential Injection Analysis. The technique gradually developed – eliminating weak points of the manifold and utilizing various monolithic or particle packed columns into the flow method for multi-component analysis getting closer to HPLC. He has utilized long-term experience with Flow Analysis to develop flow manifolds for both sample pretreatment and sample analysis, study mechanisms of chromatography, online SPE, and characterization of new SPE sorbents. The methods typically target pharmaceutical analysis, bioanalysis, and oceanography.

Present Position

Education and Practice

  • 1998-2003: Master Study at Charles University in Prague, Faculty of Pharmacy in Hradec Králové, Czech  Republic, diploma thesis on: Determination of Parabens and Sodium Diclofenac by SIA method with use of Monolithic columns 

  • 2003-2006: Ph.D. at Charles University in Prague, Faculty of Pharmacy in Hradec Králové, Department of Analytical Chemistry, Chemical Drug Control Field, Ph.D. thesis on: Sequential Injection Chromatography

  • 2003-2014: position of Pharmacist in Private Pharmacy in Vamberk, Czech Republic

  • 10/2019: Habilitated Associated Professor at Charles University in Prague, Faculty of Pharmacy in Hradec Králové, Czech  Republic, thesis: Trends in development of Sequential Injection Chromatography

Research Fellowships

  • 10/2004 - 2/2005 - ERASMUS trainee-ship – 5 months, University of Porto, Porto, Portugal (prof. Maria da Conceição B.S.M. Montenegro )

  • 10/2007 - 4/2008 - Post-Doc study stay - 7 months, University of Washington, Seattle, USA (prof. Jaromir Ruzicka)

  • 10 - 11/2013, 4 - 5/2014, 3 - 4/2015 - 3x Work stay (Bilateral cooperation Czech Republic - USA - Kontakt II) - 6 weeks, University of Hawaii at Manoa, Honolulu, USA (prof. Chris Measures, prof. Jaromir Ruzicka)

  • 9/2014 - Work stay - 1 month - Plymouth University, Plymouth, Great Britain (prof. Paul Worsfold)  

Research Interests

  • Since 2002 - Development of a new liquid chromatography area involving the application of monolithic columns in the flow methods. The proposed technique is currently being used by many researchers and practitioners around the world and commercial implemented by FIAlab® in 2007.
  • 2004 – 2005 - Development of membrane electrodes for specific potentiometric determination of B1 and B6 vitamins in the sequential injection analysis system(University of Porto)
  • Since 2007 – Development of automatic liquid phase micro-extraction method based on sequential injection analysis manifold and its hyphenation with liquid chromatography using the same manifold
  • Since 2007 – Development of Bead Injection Lab-On-Valve methods for determination of DNA by antigen interaction (University of Washington)
  • Since 2007 – Development of Bead Injection Lab-On-Valve methods for monitoring of live cell metabolism by specific fluorescence dyes and cell counting (University of Washington)
  • Since 2009 – Development of Bead Injection Lab-On-Valve methods for solid phase micro-extraction of drugs and drug metabolites from biologic samples and its hyphenation with chromatography on the sequential injection analysis manifold
  • Since 2011 -  Development of automated sequential injection analysis manifold for miniaturized solid phase extraction of substances of clinical significant in biological samples using MEPS cartridge (Micro Extraction by Packed Sorbent)
  • Since 2013 - Development of flow methods for trace metal analysis for Oceanographic and Environmental analysis


in 2021, the tutorial is further extended in 


focused mainly on advances in flow programming 


Teaching Activities

  • Analytical chemistry
  • Instrumental methods
  • Special methods of instrumental analysis
  • Supervisor of diploma, rigorous and Ph.D. projects

Grant Projects

  • 2005 - Principal investigator of project FRVŠ 191/2005/G6 „Analysis of multi-components pharmaceutical preparations using sequential injection chromatography method"
  • 2006 - Principal investigator of project FRVŠ 117/2006/G6 „Sequential injection chromatography – a new practice exercise“
  • 2005 – 2006 – Research co-worker of project GA UK 315/2005/B-CH/FaF „Use of monolithic columns in system of sequential injection chromatography for drug analysis”
  • 2005 – 2006 – Research co-worker of project GA UK 296/2005/B-CH/FaF „Use of modern separation methods for analysis of substances with potential anti-oxidation activity”
  • 2007 – 2011 – Research co-worker of project MSM 0021620822 “Research of new drug structures”
  • 2008 – 2010 – Principal investigator of Post-doc project GAČR 203/08/P338 “Using of monolithic column and LPME in sequential injection chromatography for drug analysis and biological samples pretreatment”
  • 2013 - Guarantor of project FRVŠ 1422/2013/G6 "Determination of propranolol in biological meterial with use of microexctraction by packed sorbent in systém of sequential injection analysis" 
  • 2013 - 2015 - Research co-worker of project Bilateral cooperation Czech Republic - USA - Kontakt II, "Advanced Analytical Techniques for Oceanography and Environmental Monitoring"
  • 2015 - 2017 - Research co-worker Czech Science foundation project 15-10781S – "On-line hyphenation of automated extraction processes with liquid chromatography for complete sample analysis"
  • 1/2017 – 12/2019 - Research co-worker Czech Science Foundation project 17-08738S – “Nanofiber polymers as perspective sorbents for on-line extraction in chromatography systems”

  • 4/2017 - 10/2022 - Research co-worker - Establishment of specialized team on advanced research in separation science (Reg. No. CZ.02.1.01/0.0/0.0/15_003/0000465) co-funded by ERDF

  • 5/2018 - 4/2020 - Research co-worker - Efficiency and Safety Improvement of Current Drugs and Nutraceuticals: Advanced Methods – New Challenges (EFSA–CDN) (Reg. number: CZ.02.1.01/0.0/0.0/16_019/0000841) co-funded by ERDF

Scientific Articles

42 publications (Σ IF > 145), over 700 citations, H-index 16 (Web of Science) - 1. 12. 2020

Scopus record


2 Czech Patents (co-author) - PV 2009-801 and PV 2009-726

Hobbies / Personal Interests

  • family
  • sport - tenis, mountain biking, jogging
  • traveling and hiking,
  • good music and good movies
  • photography 

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