Present position
- Assistant at the Department of Pharmaceutical Chemistry and Pharmaceutical Analysis, Faculty of Pharmacy in Hradec Králové, Charles University
- Member of the Pharmaceutical Analysis Group
Research interests
- Liquid chromatography, gas chroamtography, supercritical fluid chromatography, capillary electrophoresis
- Development of analytical and bioanalytical methods (HPLC/UHPLC-MS/MS; GC, GC-MS)
- Possibility of employment of different materials for sample preparation
- Chiral separations of biologically active compounds
- Analysis of boron cluster compounds
Education and professional carrier
2016-2018: MSc in Healthcare Bioanalytics (Specialist in Laboratory Methods) - Faculty of Pharmacy in Hradec Kralove, Charles University; (Thesis: Achiral and chiral high performance liquid chromatography of drugs and their metabolites III.)
Teaching experience
Teaching of Pharmaceutical Analysis I. and Pharmaceutical Analysis II.
Co-supervisor of 5 M.Sc. theses
Grants received
Principal investigator:
2020-2023 Grant Agency of Charles University: Analytical study of the influence of experimental conditions on the chiral separation of boron cluster compounds. (GAUK No. 168120)
Member of the research team:
2020-2024 - Specialized Team for Advanced Research on Separation Science (STARSS), Registration number of project: CZ.02.1.01/0.0/0.0/15_003/0000465
Stays
2022 - The University of Texas at Arlington, Department of Analytical Chemistry (seven months)
Professional memberships
Czech Society of Mass Spectrometry
Czech Pharmaceutical Society
Cooperation
Faculty of Pharmacy in Hradec Kralove, Charles University
Prof. Lucie Nováková, Ph.D. - Department of Analytical Chemistry – Supercritical fluid chromatography
The Czech Academy of Science
Dr. Bohumír Grüner, Ph.D. (Institute of Inorganic Chemistry, Řež) - chiral separations of born cluster compounds.
The University of Texas at Arlington
Prof. Daniel W. Armstrong, Ph. D. – Department of Chemistry and Biochemistry – Chiral separations of homoserine lactones, Chiral separations of boron cluster compounds
Publications
Author and co-author of 11 articles in international journals with IF; 21 citations (WoS); H‑index: 3 (WoS). The links to full texts are available at my ResearchGate profile.
2023
Horáček O., Portillo A.E., Dhaubhadel U., Sung Y., Readel E.R., Kučera R., Armstrong D.W. Comprehensive chiral GC-MS/MS and LC-MS/MS methods for identification and determination of N-acyl homoserine lactones. Talanta. 2023; 253: 123957. (IF2022: 6.1, Q1; AIS2022: 0.793, Q1)
Author’s contribution: investigation, data curation, writing original draft and editing, visualization.
Horáček O., Dhaubhadel U., Holub J., Grüner B., Arstrong D.W., Kučera R. Employment of chiral columns with superficially porous particles in chiral separations of cobalt bis(dicarbollide) and nido-7,8-C2B9H12(1-) derivatives. Chirality. https://doi.org/10.1002/chir.23606 [published online]. (IF2022: 2.0, Q3; AIS2022: 0.354, Q3)
Author’s contribution: investigation, data curation, conceptualization, methodology, writing original draft and editing, visualization.
Portillo A.E., Dhaubhadel U., Horáček O., Sung Y., Armstrong D.W. Investigating chirality in quorum sensing by analysis of Burkholderia cepacia and Vibrio fischeri with comprehensive chiral LC-MS/MS and GC-MS/MS methods. FEMS Microbiology Letters. 2023; 370: fnad011. (IF2022: 2.1, Q4; AIS2022: 0.582, Q3)
Author’s contribution: investigation, data curation, original draft editing.
Dhaubhadel U., Portillo A.E., Horáček O., Sung Y., Armstrong D.W. Unusual enantiomeric D,L-N-acyl homoserine lactones in Pectobacterium atrosepticum and Pseudomonas aeruginosa PLoS ONE. 2023; 18(3): 1. (IF2022: 3.7, Q2; AIS2022: 0.944, Q2)
Author’s contribution: investigation, data curation, original draft editing.
Khalikova M., Jireš J., Horáček O., Douša M., Kučera R., Nováková L. What is the role of current mass spectrometryin pharmaceutical analysis? Mass Spectrometry Reviews. 2023; : 1-50. (IF2022: 6.6, D1; AIS2022: 1.862, Q1)
Author’s contribution: writing (sections: 2.2 MS in Pharmacopoeia 2.5 The role of MS in determination of pharmaceuticals in biological fluids) and editing original draft, proofreading
Plachká K., Pilařová V., Horáček O., Gazárková T., Kočová Vlčková H., Kučera R., Nováková L. Columns in analytical-scale supercritical fluid chromatography: From traditional to unconventional chemistries. Journal of Separation Science (Accepted for Publication). (IF2022: 3.1, Q2; AIS2022: 0.371, Q3)
Author’s contribution: comprehensive literature search, writing (section: 3. Chiral stationary phases in SFC) and editing original draft, proofreading
2022
Horáček O., Nováková L., Tüzün E., Grüner B., Švec F., Kučera R. Advanced Tool for Chiral Separations of Anionic and Zwitterionic (Metalla)carboranes: Supercritical Fluid Chromatography. Analytical Chemistry. 2022; 94: 17551-17558. (IF2022: 7.4, D1; AIS2022: 1.303, Q1)
Author’s contribution: investigation, data curation, conceptualization, methodology, writing original draft and editing, visualization.
Horáček O., Marvalová J., Štilcová K., Holub J., Grüner B., Kučera R. Reversed-phase chromatography as an effective tool for the chiral separation of anionic and zwitterionic carboranes using polysaccharide-based chiral selectors. Journal of Chromatography A. 2022; 1672: 463051. (IF2022: 4.1, Q2; AIS2022: 0.553, Q2)
Author’s contribution: investigation, data curation, conceptualization, methodology, funding acquisition, writing original draft and editing, visualization.
El Anwar S., Pazderová L., Bavol D., Bakardjiev M., Ruzickova Z., Horáček O., Fojt L., Kucera R., Gruner B. Structurally rigidified cobalt bis(dicarbollide) derivatives, a chiral platform for labelling of biomolecules and new materials. Chemical Communications. 2022; 58: 2572–2575. (IF2022: 4.9, Q2; AIS2022: 1.031, Q2)
Author’s contribution: investigation – HPLC chiral separations of oxygen-bridged cobalt bis(dicarbollide) derivatives, data curation, writing the analytical part, visualization.
2021
Horáček O., Papajová-Janetková M., Grüner B., Lochman L., Štěrbová-Kovaříková P., Vespalec R., Kučera R. The first chiral HPLC separation of dicarba-nido-undecarborate anions and their chromatographic behavior. Talanta. 2021; 222: 121652. (IF2021: 6.556, Q1; AIS2021: 0.765, Q1)
Author’s contribution: investigation, data curation, methodology, writing original draft and editing, visualization.
2020
Juhás M., Kučerová M., Horáček O., Janďourek O., Kubíček V., Konečná K., Kučera R., Bárta P., Janoušek J., Paterová P., Kuneš J., Doležal M., Zitko J. N-Pyrazinoyl Substituted Amino Acids as Potential Antimycobacterial Agents-The Synthesis and Biological Evaluation of Enantiomers. Molecules. 2020; 25(7): 1-29. (IF2020: 4.412, Q2; AIS2020: 0.694, Q2)
Author’s contribution: investigation – HPLC chiral separations of N-pyrazinoyl substituted amino acids, data curation, writing the analytical part, visualization.