Burkhard Horstkotte, Ph.D., M.Sc.


Date of Birth: 6 August 1974, Hamburg, Germany
Contact Information:
Faculty of Pharmacy, Charles University
Heyrovského 1203
CZ 50005 Hradec Králové

E-mail: horstkob@faf.cuni.cz
Tel.: +420 495 067 482
Fax: +420 495 067 164


Present Position

  • Assistant professor and research associate at the Department of Analytical Chemistry,
    Faculty of Pharmacy in Hradec Králové, Charles University in Prague
  • Member of Analytical Chemistry Group (ACG)
  • Junior member of Charles University Research Centre (UNCE 204026/2012)

Education and Practice

  • 09/1995 – 08/2000 Diplom Ingenieur Umwelttechnik (Environmental Engineer), Hamburg University of Applied Sciences, Hamburg, Germany, Title of Diplom-Thesis Construction and Application of three treatment plants for the reduction of PAH and aliphatic hydrocarbons in soils by cavitation
  • 09/2004 – 09/2005 Master of Science in Environmental Engineering (M.Sc.), Hamburg University of Applied Sciences, Hamburg, Germany, Title of Master-Thesis At-line bioreactor process monitoring of formaldehyde in yeast culture medium using a multisyringe flow injection analysis device
  • 11/2008 Ph.D. in Experimental Science and Technology (Chemistry), University of the Balearic Islands (UIB), Palma de Mallorca, Spain, Title of Dissertation: Development of automated methods using syringe based flow analysis techniques and capillary electrophoresis for biotechnological process monitoring and environmental analysis

Work Experience

  • 06/1997 GKSS Research Center, Geesthacht, Germany
  • 08 – 09/1996 Laboratory Dr. Fintelmann & Dr. Meyer, Hamburg, Germany
  • 09/1998 GKSS Research Center (Helmholz Gesellschaft), Geesthacht, Germany
  • 11/1999 Labor Dr. Fintelmann & Dr. Meyer, Hamburg, Germany
  • 11/2000 – 02/2001 TÜV Nord Gesellschaft für Umweltschutz, Hamburg, Germany
  • 03/2001 – 11/2003 Federal Research Center for Fisheries, Hamburg, Germany
  • 12/2007 – 04/2009 Universidad de las Islas Baleares (UIB), Palma de Mallorca, Spain
    Group of Analytical Chemistry, Automatization, and Environment
  • 05/2009 - 04/2012 Post-Doc at the Mediterranean Institute for Advanced Studies (IMEDEA CSIC-UIB), Esporles, Spain
  • Since 05/2012 Post-Doc at the Charles University of Prague, Faculty of Pharmacy, Heyrovského 1203, Hradec Kralóvé, Czech Republic
  • 05/2012 – 12/2014 Post-Doc at the Charles University of Prague, Faculty of Pharmacy, Heyrovského 1203, Hradec Králové, Czech Republic
  • Since 01/2015 Project-contracted researcher and assistant teacher at the Charles University of Prague, Faculty of Pharmacy, Hradec Králové, Czech Republic

Research focus

  • Automation of analytical and preparative procedures using flow techniques
  • Development of automation technique In-Syringe Analysis / Lab-In-Syringe
  • Monitoring applications for environmental and oceanographic research
  • Hyphenation of flow techniques and separation techniques LC & CE

Grants received

  • 03/1999 – 07/1999 Foundation Karl-Heinz-Ditze, Hamburg University of Applied Sciences, Hamburg, Germany
  • 12/2003 – 08/2007 Conselleria d'Economia, Hisenda i Innovació, Government of the Balearic Islands, Palma de Mallorca, Spain
  • 12/2007 – 06/2009 Collaboration scholarship for project CAIB Progecic-5C, Government of the Balearic Islands, Spain
  • 05/2009 - 04/2012 JAE2009 Contract from the Spanish National Research Council (CSIC)
  • 05/2012 – 12/2014 Postdoc grant from European Project “PostDoc 1”, Charles University in Prague, Faculty of Pharmacy
  • 07/2013 – 08/2013 Grant of the University of the Balearic Islands for visiting researcher
  • 01/2014 – 06/2015 Researcher grant from European Project “TEAB”, Charles University in Prague, Faculty of Pharmacy
  • 04/2015 - 08/2015 Endeavour PostDoc, University of Melbourne


  • 2014 – Young Researcher Award from the Japanese Association of Flow Injection Analysis


Co-investigator in 13 research projects - 10 National, 3 International

Professional and research activities

  • 23 oral presentation in international conferences (7 invited)
  • 60 Poster contributions to international and national conferences


  • 43 publications in peer-reviewed journals, 41 listed in Web of Knowledge, h-index: 14, Sum of Citations: 490 (3/2017)
  • 7 publications in not peer-reviewed journals
  • Co-author of 4 book chapters
  • 1 patent (Spain) V. Cerdà & B. Horstkotte. Registration No. P201230192

Teaching activity

  • Master study Faculty of Pharmacy - Analytical chemistry, Instrumental methods, Special methods of instrumental analysis
  • (Co-)Supervisor of 4 PhD students, 6 Undergraduate students, 4 Graduate students


  • Participation in 6 Research Expeditions
  • 13 Internships at 9 Research Centers

Recommended webpages

Selected publications

  • A novel approach to Lab-In-Syringe Head-Space Single-Drop Microextraction and on-drop sensing of ammonia, I. Šrámková, B. Horstkotte, H. Sklenářová, P. Solich, S.D. Kolev, Anal. Chim. Acta 934 (2016) 132-144.

  • Automation of dispersive liquid–liquid microextraction and related techniques. Approaches based on flow, batch, flow-batch and in-syringe modes, M. Alexovič, B. Horstkotte, I. Šrámková, P. Solich, J. Sabo, Trends Anal. Chem.-TrAC 86 (2017) 39-55.

  • Fully Automatic In-Syringe Magnetic Stirring-Assisted Dispersive Liquid–Liquid Microextraction Hyphenated to High-Temperature Torch Integrated Sample Introduction System-Inductively Coupled Plasma Spectrometer with Direct Injection of the Organic Phase, R. Sánchez, B. Horstkotte, K. Fikarová, H. Sklenářová, S. Maestre, M. Miró, J.-L. Todolí, Anal. Chem., 2017, in print, DOI: 10.1021/acs.analchem.7b00400

  • Automation of static and dynamic non-dispersive liquid phase microextraction. Part 1: Approaches based on extractant drop-, plug-, film- and microflow-formation.
    Alexovic, Horstkotte, Solich, Sabo, Anal. Chim. Acta 906 (2016) 22-40.

  • Automation of static and dynamic non-dispersive liquid phase microextraction. Part 2: Approaches based on impregnated membranes and porous supports.
    Alexovic, Horstkotte, Solich, Sabo, Anal. Chim. Acta 907 (2016) 18–30.

  • Large volume preconcentration and determination of nanomolar concentrations of iron in seawater using a renewable cellulose 8-hydroquinoline sorbent microcolumn and universal approach of post-column eluate utilization in a Lab-on-Valve system.
    Horstkotte, Chocholous, Solich, Talanta 150 (2016) 213-223.

  • A Novel Approach to Lab-In-Syringe - Single Drop Microextraction and On-Drop Sensing of Ammonia.
    Šrámková, Horstkotte, Sklenářová, Solich, Kolev, Anal. Chim. Acta (2016) Accepted, Anal. Chim. Acta 934 (2016) 132–144.

  • Sequential injection analysis for automation of the Winkler methodology with real-time SIMPLEX optimization and shipboard application.
    Horstkotte, Tovar Sánchez, Duarte, Cerdà. Anal. Chim. Acta, 658 (2010) 147-155.
  • Response functions for SIMPLEX optimization of Flow Injection Analysis and related Techniques.
    Horstkotte, Duarte, Cerdà. TRAC 29 (2010) 1224-1235.
  • Improving pressure robustness, reliability and versatility of solenoid-pump flow systems using a miniature economic control unit including two simple pressure pulse mathematical models.
    Horstkotte, Ledesma, Duarte, Cerdà. Anal. Chem. 82 (2010) 6983-6990.
  • A miniature and field-applicable multipumping flow analyzer for ammonium monitoring in seawater with fluorescence detection.
    Horstkotte, Duarte, Cerdà, Talanta 85 (2011) 380-385.
  • Fully-Automated Fluorimetric Determination of Aluminium in Seawater by In-Syringe Dispersive Liquid-Liquid Microextraction using Lumogallion.
    Suárez, Horstkotte, Duarte, Cerdà. Anal. Chem. 84 (2012) 9462–9469.
  • In-Syringe-Stirring: A Novel Approach for Magnetic Stirring-Assisted Dispersive Liquid-Liquid Microextraction.
    Horstkotte, Suárez, Solich, Cerdà. Anal. Chim. Acta 788 (2013) 52-60.
  • Automated in-syringe single-drop head-space micro-extraction applied to the determination of ethanol in wine samples.
    Šrámková, Horstkotte, Solich, Sklenářová, Anal. Chim. Acta 828 (2014) 53-60.
  • In-syringe magnetic stirring-assisted dispersive liquid–liquid microextraction for automation and downscaling of methylene blue active substances assay.
    Suárez, Horstkotte, Cerdà. Talanta 130 (2014) 555-560.
  • Sequential Injection Chromatography with post-column reaction/derivatization for the determination of transition metal cations in natural water samples.
    Horstkotte, Jarošová, Chocholouš, Sklenářová, Solich. Talanta 136 (2015) 75-83.


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