Burkhard Horstkotte, Ph.D., M.Sc.


Date of Birth: 6 August 1974, Hamburg, Germany
Contact Information:
Faculty of Pharmacy, Charles University
Heyrovského 1203
CZ 50005 Hradec Králové

E-mail: horstkob@faf.cuni.cz
Tel.: +420 495 067 183
Fax: +420 495 067 164

ORCID ID: 0000-0003-2337-9634
Researcher ID: N-2739-2014
Scopus Author ID: 12241381700


Present Position

  • Assistant professor and research associate at the Department of Analytical Chemistry, Faculty of Pharmacy in Hradec Králové, Charles University, Czech Republic
  • Member of Analytical Chemistry Group (ACG)

Education and Practice

  • 09/1995 – 08/2000 Diplom Ingenieur Umwelttechnik (Environmental Engineer), Hamburg University of Applied Sciences, Hamburg, Germany, Thesis "Construction and Application of three treatment plants for the reduction of PAH and aliphatic hydrocarbons in soils by cavitation"
  • 09/2004 – 09/2005 Master of Science (M.Sc.) in Environmental Engineering, Hamburg University of Applied Sciences, Hamburg, Germany, Master-Thesis "At-line bioreactor process monitoring of formaldehyde in yeast culture medium using a multisyringe flow injection analysis device"
  • 11/2008 Ph.D. in Experimental Science and Technology (Chemistry), University of the Balearic Islands (UIB), Palma de Mallorca, Spain, Title of Dissertation: "Development of automated methods using syringe based flow analysis techniques and capillary electrophoresis for biotechnological process monitoring and environmental analysis"

Work Experience

  • 06/1997 GKSS Research Center, Geesthacht, Germany
  • 08 – 09/1996 Laboratory Dr. Fintelmann & Dr. Meyer, Hamburg, Germany
  • 09/1998 GKSS Research Center (Helmholz Gesellschaft), Geesthacht, Germany
  • 11/1999 Labor Dr. Fintelmann & Dr. Meyer, Hamburg, Germany
  • 11/2000 – 02/2001 TÜV Nord Gesellschaft für Umweltschutz, Hamburg, Germany
  • 03/2001 – 11/2003 Federal Research Center for Fisheries, Hamburg, Germany
  • 12/2007 – 04/2009 University of the Balearic Islands, Palma de Mallorca, Spain
    Group of Analytical Chemistry, Automatization, and Environment
  • 05/2009 - 04/2012 Post-Doc at the Mediterranean Institute for Advanced Studies (IMEDEA - CSIC & UIB), Esporles, Spain
  • 05/2012 – 12/2014 Post-Doc at the Charles University of Prague, Faculty of Pharmacy, Heyrovského 1203, Hradec Králové, Czech Republic
  • 2014 - 2017 Junior member of Charles University Research Centre (UNCE 204026/2012)
  • 01/2015 - 8/2017 Project-contracted researcher and assistant teacher at the Charles University of Prague, Faculty of Pharmacy, Hradec Králové, Czech Republic

Research focus

  • Automation of analytical and preparative procedures using flow techniques (Head space extraction, single drop microextraction, DLLME, and SPE)
  • Development of the automation technique Lab-In-Syringe / In-Syringe Analysis
  • Monitoring applications for environmental and oceanographic research
  • Hyphenation of flow techniques and separation techniques LC & CE and advanced detection techniques (ICP-MS, ICP-OES)
  • 3D printing and prototyping of flow system components


  • 2014 – Young Researcher Award from the Japanese Association of Flow Injection Analysis

Grants received

  • 03/1999 – 07/1999 Foundation Karl-Heinz-Ditze, Hamburg University of Applied Sciences, Hamburg, Germany
  • 12/2003 – 08/2007 Conselleria d'Economia, Hisenda i Innovació, Government of the Balearic Islands, Palma de Mallorca, Spain
  • 12/2007 – 06/2009 Collaboration scholarship for project CAIB Progecic-5C, Government of the Balearic Islands, Spain
  • 05/2009 - 04/2012 JAE2009 Contract from the Spanish National Research Council (CSIC)
  • 05/2012 – 12/2014 Postdoc grant from European Project “PostDoc 1”, Charles University in Prague, Faculty of Pharmacy
  • 3/2013 - 12/2015 Bilateral cooperation Czech Republic - USA - Kontakt II, "Advanced Analytical Techniques for Oceanography and Environmental Monitoring"
  • 07/2013 – 08/2013 Grant of the University of the Balearic Islands for visiting researcher
  • 01/2014 – 06/2015 Researcher grant from European Project “TEAB”, Charles University in Prague, Faculty of Pharmacy
  • 04/2015 - 08/2015 Endeavour PostDoc, University of Melbourne


Co-investigator in 14 research projects - 11 National, 3 International

Teaching activity

  • Master study Faculty of Pharmacy - Analytical chemistry, Instrumental methods, Special methods of instrumental analysis
  • (Co-)Supervisor of 4 PhD students, 6 Undergraduate students, 4 Graduate students and 3 international Erasmus students

Collaborations within the last 3 years

  • Prof. M. Miró Lladò, FI-Trace Group, University of the Balearic Islands, Spain
  • Prof. J. Dědina, Akademie of Sciences, Insitute of Analytical Chemistry, Prague, Czech Republic
  • Dr. M. Alexovič, Faculty of Medicine, University of P. J. Šafárik in Košice, Slovakia
  • Prof. Dr. J.-L. Todolí, University of Alicante, Dep. Anal. Chem., Spain
  • Prof. Dr. E.L. de Moraes Flores, Federal University of Technology of Paraná, Brazil
  • Prof. S.D. Kolev, School of Chemistry, University of Melbourne, Australia
  • Prof. Dr. C.C. Acebal, INQUISUR, Dep. Chem. Universidad Nacional del Sur, Bahia Blanca, Argentina

Conference contributions

  • 24 oral presentation in international conferences (7 invited)
  • 63 Poster contributions to international and national conferences


  • 47 publications in peer-reviewed journals, 45 listed in Web of Knowledge, h-index: 16, Sum of Citations: >600 (3/2018), ORCID ID: 0000-0003-2337-9634
  • 7 publications in not peer-reviewed journals
  • Co-author of 4 book chapters
  • 1 patent (Spain) V. Cerdà & B. Horstkotte. Registration No. P201230192

Selected publications

  • Online Coupling of fully automatic in-syringe dispersive liquid-liquid microextraction with oxidative back-extraction to inductively coupled plasma spectrometry for sample clean-up in elemental analysis: A Proof of Concept, B. Horstkotte, K. Fikarová, D.J. Cocovi-Solberg, H. Sklenářová, P. Solich, M. Miró, Talanta 173 (2017) 79-87.
  • Automation of dispersive liquid–liquid microextraction and related techniques. Approaches based on flow, batch, flow-batch and in-syringe modes, M. Alexovič, B. Horstkotte, I. Šrámková, P. Solich, J. Sabo, Trends Anal. Chem. 86 (2017) 39-55.
  • A novel approach to Lab-In-Syringe Head-Space Single-Drop Microextraction and on-drop sensing of ammonia, I. Šrámková, B. Horstkotte, H. Sklenářová, P. Solich, S.D. Kolev, Anal. Chim. Acta 934 (2016) 132-144.
  • Fully Automatic In-Syringe Magnetic Stirring-Assisted Dispersive Liquid–Liquid Microextraction Hyphenated to High-Temperature Torch Integrated Sample Introduction System-Inductively Coupled Plasma Spectrometer with Direct Injection of the Organic Phase, R. Sánchez, B. Horstkotte, K. Fikarová, H. Sklenářová, S. Maestre, M. Miró, J.-L. Todolí, Anal. Chem. 89 (2017) 3787-3794.
  • Automation of static and dynamic non-dispersive liquid phase microextraction. Part 1: Approaches based on extractant drop-, plug-, film- and microflow-formation. Alexovic, Horstkotte, Solich, Sabo, Anal. Chim. Acta 906 (2016) 22-40.
  • Automation of static and dynamic non-dispersive liquid phase microextraction. Part 2: Approaches based on impregnated membranes and porous supports. Alexovic, Horstkotte, Solich, Sabo, Anal. Chim. Acta 907 (2016) 18-30.
  • Large volume preconcentration and determination of nanomolar concentrations of iron in seawater using a renewable cellulose 8-hydroquinoline sorbent microcolumn and universal approach of post-column eluate utilization in a Lab-on-Valve system. Horstkotte, Chocholous, Solich, Talanta 150 (2016) 213-223.
  • A Novel Approach to Lab-In-Syringe - Single Drop Microextraction and On-Drop Sensing of Ammonia. Šrámková, Horstkotte, Sklenářová, Solich, Kolev, Anal. Chim. Acta 934 (2016) 132–144.
  • Automated in-syringe single-drop head-space micro-extraction applied to the determination of ethanol in wine samples. Šrámková, Horstkotte, Solich, Sklenářová, Anal. Chim. Acta 828 (2014) 53-60.
  • In-syringe magnetic stirring-assisted dispersive liquid–liquid microextraction for automation and downscaling of methylene blue active substances assay. Suárez, Horstkotte, Cerdà. Talanta 130 (2014) 555-560.
  • In-Syringe-Stirring: A Novel Approach for Magnetic Stirring-Assisted Dispersive Liquid-Liquid Microextraction. Horstkotte, Suárez, Solich, Cerdà. Anal. Chim. Acta 788 (2013) 52-60.
  • Fully-Automated Fluorimetric Determination of Aluminium in Seawater by In-Syringe Dispersive Liquid-Liquid Microextraction using Lumogallion. Suárez, Horstkotte, Duarte, Cerdà. Anal. Chem. 84 (2012) 9462–9469.


  • Participation in 6 Research Expeditions
  • 13 Internships at 9 Research Centers

Recommended webpages

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