Assoc. Prof. Petra Kovaříková, Ph.D.

Research interests

• Development of LC-MS methods for targeted assay of novel drugs and the metabolites in biological materials, stability of drugs/drug candidates in biological environments
• Bioanalytical study on pharmacokinetics and biotransformation of novel thiosemicarbazone anti-cancer agents
• Bioanalysis and bioactivation of novel cardioprotective drugs
• Sample preparation prior LC-MS analysis of drugs

Education and professional carrier

• Since 2012: associate professor of Pharmaceutical Chemistry, Faculty of Pharmacy in Hradec Kralove, Charles University in Prague, thesis: High-performance liquid chromatography in the analysis of clinically used drugs and novel drug candidates
• 2004-2012: researcher at the Department of Pharmaceutical Chemistry and Drug Analysis, Faculty of Pharmacy in Hradec Kralove, Charles University in Prague
• 2005-2011: researcher of the center “Research of new drugs“- Faculty of Pharmacy in Hradec Kralove, Charles University in Prague
• 2001-2006: PhD in Pharmaceutical Analysis - Faculty of Pharmacy in Hradec Kralove, Charles University in Prague, thesis: Analytical evaluation of drugs and drug candidates using HPLC
• 1996-2001: MSc in Pharmacy - Faculty of Pharmacy in Hradec Kralove, Charles University in Prague, thesis: The chromatographic methods in the analysis of drugs

Teaching experience

• Mentor of 1 post-doctoral research associate and 4 PhD students
• Supervisor of 1 Bc. and 30 MS.c theses
• Teaching of Pharmaceutical Analysis, Advanced Pharmaceutical Analysis, Bioanalysis of Drugs, Mass Spectrometry

Grants received (principal investigator)

• 2011-2013: Ministry of Health (NT12403-3/2011): “Pharmaceutical analysis, pharmacokinetics and biotransformation study of novel thiosemicarbazone anticancer agents”
• 2012: Ministry of Education (366/2012): “Innovation and development of instrumental laboratories for drug analysis”
• 2010-2012: Czech Science Foundation, post-doc. project (P206/10P439): “Modern bioanalytical approach to interdisciplinary investigation of bisdioxopiperazine cardioprotective agents”
• 2006: Ministry of Education (1319/2006): “Innovation of the subjects Drug Analysis and Therapeutic Drug
• 2005: Charles University (284/2006): “Pharmaceutical-analytical approach to evaluation of novel drug candidates from the group of iron chelators” of iron chelators”
• 2004: Charles University (395/2004): “Development of HPLC evaluation of novel drug candidates from the group of pyridoxal isonicotinoyl hydrazone analogues and the application to the analysis of biological materials”
• 2004: Ministry of Education (952/2004): “Stability study of pyridoxal isonicotinoyl hydrazone”

Other: Co-investigator-Czech Science Foundation (2x), supervision of 4 student grants of Charles University, University Centre for Research of Toxic and Protective Effects of Drugs on Cardiovascular System

Professional membership

• Czech Society of Mass Spectrometry

Cooperation

• Prof. PharmDr. Tomáš Šimůnek, Ph.D. -  In vitro investigation of bioactivation of cardioprotective agents
• Assoc.PharmDr. Jaroslav Roh, Ph.D., Assoc. Prof. PharmDr. Kateřina Vávrová, Ph.D.   – Chemical synthesis of metabolites

• Assoc. Prof. PharmDr. Martin Štěrba, Ph.D. -  Pharmacokinetics and bioactivation of cardioprotective agents
• Prof. MuDr. Stanislav Mičuda, Ph.D.- Pharmacokinetics of thiosemicarbazone anticancer drugs

• Prof. Des R. Richardson -  Development of novel thiosemicarbazone anticancer drugs  - pharmacokinetics and biotransformation study

• Assoc. Prof. Katherine J. Franz - Development of boronate prochelators

Publications

Author and co-author of 47 articles in international journals with IF; 584 citations (WoS; excluding self-citations); H-index: 15 (WoS).

Selected articles (2009-2019):

• Reimerová P., Stariat J., Bavlovič Piskáčková H., Jansová H., Roh J., Kalinowski D.S., Macháček M., Šimůnek T., Richardson D.R., Štěrbová-Kovaříková P. Novel SPME fibers based on a plastic support for determination of plasma protein binding of thiosemicarbazone metal chelators: a case example of DpC, an anti-cancer drug that entered clinical trials. Analytical and Bioanalytical Chemistry. 2019;. doi: 10.1007/s00216-019-01681-w. (IF2017=3.307)

• Reimerová P., Jirkovská A., Bavlovic Piskackova H., Karabanovich G., Roh J., Šimůnek T., Štěrbová-Kovaříková P. UHPLC-MS/MS method for analysis of sobuzoxane, its active form ICRF-154 and metabolite EDTA-diamide and its application to bioactivation study. Scientific Reports. 2019; 9(1):4524 (IF₂₀₁₇=4.122)

• Jansová H., Kubeš J., Reimerová P., Štěrbová-Kovaříková P., Roh J., Šimůnek T. 2,6-Dihydroxybenzaldehyde Analogues of the Iron Chelator Salicylaldehyde Isonicotinoyl Hydrazone: Increased Hydrolytic Stability and Cytoprotective Activity against Oxidative Stress. Chemical Research in Toxicology. 2018; 31(11):1151-1163. (IF₂₀₁₇=3.432)

• Hrušková K,. Potůčková E., Opálka L., Hergeselová T., Hašková P., Kovaříková P., Šimůnek T., Vávrová K. Structure-Activity Relationships of Nitro-Substituted Aroylhydrazone Iron Chelators with Antioxidant and Antiproliferative Activities. Chemical Research in Toxicology. 2018;31(6):435-446 (IF₂₀₁₇=3.432)

• Jirkovský E., Jirkovská A., Bureš J., Chládek J., Lenčová O., Stariat J., Pokorná Z., Karabanovich G., Roh J., Brázdová P., Šimůnek T., Kovaříková P., Štěrba M. Pharmacokinetics of the Cardioprotective Drug Dexrazoxane and Its Active Metabolite ADR-925 with Focus on Cardiomyocytes and the Heart. Journal of Pharmacology and Experimental Therapeutics. 2018;364:433-446. (IF₂₀₁₇= 3.706)

• Bures J., Jirkovska A., Sestak V., Jansova H., Karabanovich G., Roh J., Sterba M., Simunek T., Kovarikova P. Investigation of novel dexrazoxane analogue JR-311 shows significant cardioprotective effects through topoisomerase IIbeta but not its iron chelating metabolite. Toxicology. 2017;392:1-10. (IF₂₀₁₇=3.265)

• Sestak V., Roh J., Klepalova L., Kovarikova P. A UHPLC-UV-QTOF study on the stability of carfilzomib, a novel proteasome inhibitor, Journal of Pharmaceutical and Biomedical Analysis. 2016; 124: 365-73 (IF₂₀₁₆=3.255)

• Jansová H., Bureš J., Macháček M., Hašková P., Jirkovská A., Roh J., Wang Q., Franz K.J., Kovaříková P., Šimůnek T. Characterization of cytoprotective and toxic properties of iron chelator SIH, prochelator BSIH and their degradation products, Toxicology. 2016; 350-352:15-24 (IF₂₀₁₆=3.582)

• Hrušková K., Potůčková E., Hergeselová T., Liptáková L., Hašková P., Mingas P., Kovaříková P., Šimůnek T., Vávrová K. Aroylhydrazone iron chelators: Tuning antioxidant and antiproliferative properties by hydrazide modifications, European Journal of Medicinal Chemistry. 2016; 120:97-110 (IF₂₀₁₆=4.519)

• Tycova A., Vido M., Kovarikova P., Foret F. Interface-free capillary electrophoresis-mass spectrometry system with nanospray ionization-Analysis of dexrazoxane in blood plasma, Journal of Chromatography A. 2016; 1466:173-9. (IF₂₀₁₆=3.891)

• Lenčová-Popelová O., Jansová H., Jirkovský E., Bureš J., Jirkovská-Vávrová A., Mazurová Y., Reimerová P., Vostatková L., Adamcová M., Hroch M., Pokorná Z., Kovaříková P., Šimůnek T, Štěrba M. Are cardioprotective effects of NO-releasing drug molsidomine translatable to chronic anthracycline cardiotoxicity settings? Toxicology. 2016; 372:52-63. (IF₂₀₁₆=3.582)

• Hašková P., Jansová H., Bureš J., Macháček M., Jirkovská A., Franz K.J., Kovaříková P., Šimůnek T.Cardioprotective effects of iron chelator HAPI and ROS-activated boronate prochelator BHAPI against catecholamine-induced oxidative cellular injury. Toxicology. 2016; 371:17-28. (IF₂₀₁₆=3.582)

• Šesták V., Stariat J., Cermanová J., Potůčková E., Chládek J., Roh J., Bureš J., Jansová H., Průša P., Stěrba M., Mičuda S., Šimůnek T., Kalinowski D. S., Richardson D.R., Kovaříková P. Novel and Potent Anti-Tumor and Anti-Metastatic Di-2-Pyridylketone Thiosemicarbazones Demonstrate Marked Differences in Pharmacology Between the First and Second Generation Lead Agents, Oncotarget. 2015; 6(40):42411-28 (IF₂₀₁₅=5.008)

• Potůčková E., Roh J., Macháček M., Sahni S., Stariat J., Šesták V., Jansová H., Hašková P., Jirkovská A., Vávrová K., Kovaříková P., Kalinowski D. S., Richardson D. R., Šimůnek T. In vitro characterization of the pharmacological properties of the anti-cancer chelator, Bp4eT, and ts phase I metabolites, PLoS ONE. 2015; 10(10): e0139929 (IF₂₀₁₅=3.057)

• Bureš J., Jansová H., Stariat J., Filipský T., Mladěnka P., Šimůnek T., Kučera R., Klimeš J., Wang Q., Franz K.J., Kovaříková P. LC-UV/MS methods for the analysis of prochelator-boronyl salicylaldehyde isonicotinoyl hydrazone (BSIH) and its active chelator salicylaldehyde isonicotinoyl hydrazone (SIH). Journal of Pharmaceutical and Biomedical Analysis. 2015; 105(1): 55-63. (IF₂₀₁₅= 3.169)

• Potůčková E., Hrušková K., Bureš J., Kovaříková P., Špirková I.A., Pravdíková K., Kolbabová L., Hergeselová T., Hašková .P, Jansová H, Macháček M., Jirkovská A., Richardson V., Lane D.J., Kalinowski D.S., Richardson D.R., Vávrová K., Šimůnek T. Structure-activity relationships of novel salicylaldehyde isonicotinoyl hydrazone (SIH) analogs: iron chelation, anti-oxidant and cytotoxic properties. PLoS One. 2014, 9(11): e112059.  (IF₂₀₁₄= 3.232)

• Cermanova J., Kadova Z., Dolezelova E., Zagorova M., Safka V., Hroch M., Laho T., Holeckova M., Mokry J., Kovarikova P., Bures J., Sterba M., Micuda S. Deferoxamine but not dexrazoxane alleviates liver injury induced by endotoxemia in rats. Shock. 2014, 42(4):372-79. (IF₂₀₁₄= 3.045)

• Kučera R., Kovaříková P., Pasáková-Vrbatová .I, Slaninová J., Klimeš J. Zirconia--a stationary phase capable of the separation of polar markers of myocardial metabolism in hydrophilic interaction chromatography. Journal of Separation Science. 2014, 37(9-10):1089-93. (IF₂₀₁₃= 2.594)

• Stariat J., Suprunová V., Roh J., Šesták V., Eisner T., Filipský T., Mladěnka P., Nobilis M., Šimůnek T., Klimeš J., Kalinowski D.S., Richardson D.R., Kovaříková P. Simultaneous determination of the novel thiosemicarbazone anti-cancer agent, Bp4eT, and its main phase I metabolites in plasma: Application to a pilot pharmacokinetic study in rats. Biomedical Chromatography. 2014; 28(5):621-9 (IF₂₀₁₃= 1.662)

• Stariat J., Kovaříková P., Kučera R., Klimeš J., Kalinowski D.S, Richardson D.R, Ketola R.A. Identification of in vitro metabolites of the novel anti-tumor thiosemicarbazone, DpC, using ultra-high performance liquid chromatography-quadrupole-time-of-flight mass spectrometry, Analytical and Bioanalytical Chemistry. 2013; 405(5):1651-61. (IF₂₀₁₃= 3.578)

• Kovaříková P., Pasaková-Vrbatová I., Vávrová A., Stariat J., Klimeš J., Simunek T. Development of LC–MS/MS method for the simultaneous analysis of the cardioprotective drug dexrazoxane and its metabolite ADR-925 in isolated cardiomyocytes and cell culture medium, Journal of Pharmaceutical and Biomedical Analysis .2013; 76(1): 243-51. (IF₂₀₁₃= 2.829)

• Štěrba M., Popelová O., Vávrová A., Jirkovský E., Kovaříková P., Geršl V., Šimůnek T. Oxidative stress, redox signaling and metal chelation in anthracycline cardiotoxicity and pharmacological cardioprotection, Antioxidants & Redox Signaling. 2013; 18(8): 899-929. (IF₂₀₁₃: 7.667)

• Macková E., Hrušková K., Bendová P., Vávrová A., Jansová H., Hašková P., Kovaříková P., Vávrová K., Šimůnek T. Methyl and ethyl ketone analogs of salicylaldehyde isonicotinoyl hydrazone: Novel iron chelators with selective antiproliferative action. Chemico-Biological Interactions. 2012; 197: 69-79. (IF₂₀₁₁: 2.865)

• Stariat J., Šesták V., Vávrová K., Nobilis M., Kollárová Z., Klimeš J., Kalinowski D.S., Richardson D.R., Kovaříková P. LC-MS/MS identification of the principal in vitro and in vivo phase I metabolites of the novel thiosemicarbazone anti-cancer drug, Bp4eT, Analytical and Bioanalytical Chemistry. 2012; 403(1): 309-321. (IF₂₀₁₁: 3.778)

• Vávrová K., Kovaříková P., Školová B., Líbalová M., Roh J., Čáp R., Holý A., Hrabálek A. Enhanced Topical and Transdermal Delivery of Antineoplastic and Antiviral Acyclic Nucleoside Phosphonate cPr-PMEDAP. Pharmaceutical Research. 2011; 28(12): 3105-3115.  (IF₂₀₁₀: 4.456)

• Hašková P., Koubková L., Vávrová A., Macková E., Hrušková K., Kovaříková P., Vávrová K., Šimůnek T. Comparison of various iron chelators used in clinical practice as protecting agents against catecholamine-induced oxidative injury and cardiotoxicity. Toxicology. 2011; 28(2-3): 122-131. (IF₂₀₁₁: 3.681)

• Kučera R., Kovaříková P., Klivický M., Klimeš J. The retention behaviour of polar compounds on zirconia based stationary phases under hydrophilic interaction liquid chromatography conditions, Journal of Chromatography A. 2011; 1218(39): 6981-6986. (IF₂₀₁₁: 4.531)

• Pasáková I., Gladziszová M., Charvátová J., Stariat J., Klimeš J., Kovaříková P. Use of different stationary phases for separation of isoniazid, its metabolites and vitamin B6 forms, Journal of Separation Science. 2011; 34(12): 1357-1365. (IF₂₀₁₁: 2.733)

• Hrušková K., Kovaříková P., Bendová P., Hašková P., Macková E., Stariat J., Vávrová A., Vávrová K., Šimůnek T. Synthesis and initial in vitro evaluations of novel antioxidant aroylhydrazone iron chelators with increased stability against plasma hydrolysis, Chemical Research in Toxicology. 2011; 24(3): 290-302. (IF₂₀₁₁: 3.779)

• Hašková P., Kovaříková P., Koubková L., Vávrová A., Macková E., Šimůnek T. Iron chelation with salicylaldehyde isonicotinoyl hydrazone (SIH) protects against catecholamine autooxidation and cardiotoxicity, Free Radical Biology and Medicine. 2011; 50(4): 537-549. (IF₂₀₁₁: 5.423)

• Kovaříková P., Stariat J., Klimeš J., Hrušková K., Vávrová K. Hydrophilic interaction liquid chromatography in the separation of a moderately lipophilic drug from its highly polar metabolites - The cardioprotectant dexrazoxane as a model case, Journal of Chromatography A. 2011; 1218(3): 416-426. (IF₂₀₁₁: 4.531)

• Bendová P., Macková E., Hašková P., Vávrová A., Jirkovský E., Šterba M., Popelová O., Kalinowski D.S, Kovaříková P., Vávrová K., Richardson D.R, Šimůnek T. Comparison of clinically used and experimental iron chelators for protection against oxidative stress-induced cellular injury, Chemical Research in Toxicology. 2010; 23(6): 1105-1114. (IF₂₀₁₀: 4.140)

• Stariat J., Kovaříková P., Klimeš J., Kalinowski D.S., Richardson D.R. Development of an LC-MS/MS method for analysis of interconvertible Z/E Isomers of novel anti-cancer agent, Bp4eT, Analytical and Bioanalytical Chemistry. 2010; 397(1): 161-171. (IF₂₀₁₀: 3.841)

• Pasáková I., Kovaříková P., Kučera R., Klimeš J., Sochor J., Hrabálek A. Development and validation of an LC-ESI-MS ion-trap method for analysis of impurities in Transkarbam 12, a novel transdermal accelerant Chromatographia. 2009; 96(9-10): 977-983. (IF₂₀₀₉: 1.098)

• Novotný J., Kovaříková P., Novotný M., Janušová B., Hrabálek A., Vávrová K. Dimethylamino acid esters as biodegradable and reversible transdermal permeation enhancers: Effects of linking chain length, chirality and polyfluorination, Pharmaceutical Research. 2009; 26(4): 811 – 821. (IF₂₀₀₉: 3.933)

• Stariat J., Kovaříková P., Klimeš J., Lovejoy D.B., Kalinowski D.S., Richardson D.R. HPLC methods for determination of two novel thiosemicarbazone anti-cancer drugs (N4mT and Dp44mT) in plasma and their application to in vitro plasma stability of these agents, Journal of Chromatography B. 2009; 877(3): 316 – 322. (IF₂₀₀₉: 2.777)