Assoc. Prof. Radim Kučera, Ph.D.

Present position

  • Associate professor at  the Department of Pharmaceutical Chemistry and Pharmaceutical Analysis, Faculty of Pharmacy in Hradec Králové, Charles University
  • Member of the Pharmaceutical Analysis Group (Group Leader)

Contact information:
Faculty of Pharmacy, Heyrovského 1203, 500 05 Hradec Králové, Czech Republic
Tel: +420 495067446

ORCID ID 0000-0003-3052-7965; ResearcherID: S-6366-2017; Scopus Author ID: 56250821200

Research interests

  • Separation techniques – HPLC, GC, CE
  • Use of alternative stationary phases in pharmaceutical analysis
  • Possibility of employment of different materials for sample preparation
  • Method development of analytical and bioanalytical methods (HPLC/UHPLC-MS/MS; GC, GC-MS)               

Education and professional carrier

  • Since 2015: associate professor of Pharmaceutical Chemistry, Faculty of Pharmacy in Hradec Kralove, Charles University; (Thesis: Utilization of alternative stationary phases in the analysis of biologically active compounds)
  • 2002-2012: researcher at the Department of Pharmaceutical Chemistry and Pharmaceutical Analysis, Faculty of Pharmacy in Hradec Kralove, Charles University
  • 2005-2011: researcher of the centere “Research of new drugs“- Faculty of Pharmacy in Hradec Kralove, Charles University
  • 2001-2006: PhD in Pharmaceutical Analysis - Faculty of Pharmacy in Hradec Kralove, Charles University; (Thesis: Zirconia-based and chiral stationary phases in pharmaceutical analysis )
  • 1996-2001: MSc in Pharmacy - Faculty of Pharmacy in Hradec Kralove, Charles University; (Thesis: HPLC analysis of antirheumatic drugs V.)

Teaching experience

  • Teaching of Pharmaceutical Analysis I. and Pharmaceutical Analysis II., Advanced Pharmaceutical Analysis, Analysis of Exogenous Compounds in Biological Material, Bioanalysis of Drugs, Mass Spectrometry
  • Supervisor of 3 PhD students, Co-supervisor of 3 PhD students
  • Supervisor of 32 M.Sc. theses

Grants received

Principal investigator:

  • 2006 FRVŠ: “The use of head-space gas chromatography in the drug control” (388/2006)
  • 2004 FRVŠ: “The chiral separation in drug analysis using electromigration methods“ (938/2004)

Tutor of the principal investigator:

  • 2021-2024  Grant Agency of Charles University: Complex approach to the development of a separation method for the research of catecholamines in a placenta (GAUK No. 349521)
  • 2020-2023  Grant Agency of Charles University: Analytical study of the influence of experimental conditions on the chiral separation of boron cluster compounds. (GAUK No. 168120)
  • 2012-2014 Grant Agency of Charles University: “Possibilities of metal oxide-based materials in sample preparation and separation of complex analytes”
  • 2009 FRVŠ: “The employment of zirconia-based stationary phases in drug analysis” (1081/2009)
  • 2006-2007 Grant Agency of Charles University: “The possibilities of employment of modern HPLC stationary phases in the pharmaceutical analysis”

Member of the research team:

  • 2020-2024 AZV - Placental tryptophan metabolism linking maternal inflammation and foetal neurodevelopmental disorders.

  • 2017-2019 Czech Science Foundation: Azaphthalocyanines – dark quenchers in DNA hybridization probes. (GAČR: 17-19094S)

  • 2012-2014 Charles University: UNCE project “Centre for investigation of toxic and protective effects of drugs on cardiovascular system” (UNCE 33/2012).
  • 2013 FRVŠ: "Innovation and development of laboratory for practical training in pharmaceutical analysis and bioanalysis"
  • 2012 FRVŠ: “Innovation and development of instrumental laboratories for drug analysis”
  • 2012-2013 Internal Grant Agency of Ministry of Health: “Pharmaceutical analysis, pharmacokinetic and biotransformation study of novel thiosemicarbazone anticancer drugs.“
  • 2006: FRVŠ: “Innovation of the laboratory for practical training in pharmaceutical-analytical instrumental methods” (668/2006)
  • 2005-2011 Ministry of Education, Youth and Sports: Institutional research plan “Research of novel drug structures” (MSM0021620822).


  • 2002 - 2004 - R&D department, Zentiva, Prague, Czech Republic (two months)
  • 2003 - University of Greifswald, Faculty of Mathematics and Natural Sciences, Institute of Pharmacy, Germany (one month)
  • 2012 - University of Greifswald, Faculty of Mathematics and Natural Sciences, Institute of Pharmacy, Germany (one month)

Professional membership

  • Czech Society of Mass Spectrometry
  • Czech Pharmaceutical Society


Faculty of Pharmacy in Hradec Kralove, Charles University

  • Prof. František Štaud, Ph.D. - Department of Pharmacology and Toxicology - Tryptophan and serotonin in the placenta
  • Assoc. Prof. PharmDr. Martina Čečková, Ph.D. - Department of Pharmacology and Toxicology - Interactions of cyclindependent kinases with ABC drug transporters
  • Prof. Petr Zimčík, Ph.D. - Department of Pharmaceutical Chemistry and Pharmaceutical Analysis – Analysis of oligonucleotide probes

The Czech Academy of Science

  • Dr. Bohumír Grüner, Ph.D. (Institute of Inorganic Chemistry, Rez) and Assoc. Prof. Radim Vespalec, Dr.Sc. (Institute of Biofysics, Brno)-  chiral separability of born cluster compounds.



Author and co-author of 45 articles in international journals with IF; 464 citations (WoS; excluding self-citations); H-index: 13 (WoS). The links to full texts are available at the Pharmaceutical Analysis Group website.


  • Halaskova M., Rahali A., Almeida-Marrero V., Machacek M., Kučera R., Jamoussi B., Torres, T., Novakova V., de la Escosura A., Zimcik P. Peripherally Crowded Cationic Phthalocyanines as Efficient Photosensitizers for Photodynamic Therapy. ACS Medical Chemistry Letters. 2021; 12: 502-507. (IF2019=3.975)
  • Kučera R., Scensna A., Miletin M., Zimcik P. The chromatographic behaviour of new double-labelled oligodeoxynucleotide probes containing azaphthalocyanine dye as a quencher with respect to evaluation of their purity. Biomedical Chromatography. 2021; 35: e5033.  (IF2019=1.728)
  • Horáček O., Papajová-Janetková M., Grüner B., Lochman L., Štěrbová-Kovaříková P., Vespalec R., Kučera R. The first chiral HPLC separation of dicarba-nido-undecarborate anions and their chromatographic behavior. Talanta. 2021; 222: 121652. (IF2019=5.339)


  • Abad C., Karahoda R., Kastner P., Portillo R., Horackova H., Kučera R., Nachtigal P., Staud F. Profiling of Tryptophan Metabolic Pathways in the Rat Fetoplacental Unit during Gestation. International Journal of Molecular Sciences. 2020; 21: 7578. (IF2019=4.556)
  • Karahoda R., Abad C., Horackova H., Kastner P., Zaugg J., Cerveny L., Kučera R., Albrecht Ch., Staud F. Dynamics of Tryptophan Metabolic Pathways in Human Placenta and Placental-Derived Cells: Effect of Gestation Age and Trophoblast Differentiation. Frontiers in Cell and Developmental Biology. 2020; 8: 1-15. (IF2019=5.186)
  • Kollar J., Machacek M., Halaskova M., Lenco J., Kučera R., Demuth J., Rohlickova M., Hasonova K., Miletin M., Novakova V., Zimcik P. Cationic Versus Anionic Phthalocyanines for Photodynamic Therapy: What a Difference the Charge Makes. Journal of Medicinal Chemistry. 2020; 63: 7616-7632. (IF2019=6.205)
  • Karahoda R., Horackova H., Kastner P., Matthios A., Cerveny L., Kučera R., Kacerovsky M., Tebbens J. D., Bonnin A., Abad C., Staud F. Serotonin homeostasis in the materno-foetal interface at term: Role of transporters (SERT/SLC6A4 and OCT3/SLC22A3) and monoamine oxidase A (MAO-A) in uptake and degradation of serotonin by human and rat term placenta. Acta Physiologica. 2020; 229(4): 1-16. (IF2019=5.542)
  • Juhás M., Kučerová M., Horáček O., Janďourek O., Kubíček V., Konečná K., Kučera R., Bárta P., Janoušek J., Paterová P., Kuneš J., Doležal M., Zitko J. N-Pyrazinoyl Substituted Amino Acids as Potential Antimycobacterial Agents-The Synthesis and Biological Evaluation of Enantiomers. Molecules. 2020; 25(7): 1-29. (IF2019=3.267)
  • Demuth J., Miletin M., Kucera R., Ruzicka A., Havlinova Z., Libra A., Novakova V., Zimcik P. Self-assembly of azaphthalocyanine-oligodeoxynucleotide conjugates into J-dimers: towards biomolecular logic gates. Organic Chemistry Frontiers. 2020; 7: 445-456. (IF2019=5.155)


  • Kollar J., Machacek M., Jancarova A., Kubat P., Kucera R., Miletin M., Novakova V., Zimcik P. Effect of bovine serum albumin on the photodynamic activity of sulfonated tetrapyrazinoporphyrazine. Dyes and Pigments. 2019; 162: 358-366. (IF2019= 4.613)


  • Sorf A., Hofman J., Kučera R., Staud F., Ceckova M.  Ribociclib shows potential for pharmacokinetic drug-drug interactions being a substrate of ABCB1 and potent inhibitor of ABCB1, ABCG2 and CYP450 isoforms in vitro. Biochemical Pharmacology. 2018; 154: 10-17. (IF2017=4.235)
  • Demuth J., Kučera R., Kopecky K., Havlinova Z., Libra A., Novakova V., Miletin M., Zimcik P. Efficient Synthesis of a Wide-Range Absorbing Azaphthalocyanine Dark Quencher and Its Application to Dual-Labeled Oligonucleotide Probes for Quantitative Real-Time Polymerase Chain Reactions. Chemistry-A European Journal. 2018; 24(38): 9658-9666. (IF2017=5.160)


  • Klivický M., Klimeš J., Kučera R. Solid-Phase Extraction of Ibuprofen from Pharmaceuticals via Ligand Exchange Using Zirconium Dioxide. Current Analytical Chemistry. 2016; 12(6): 523-528. (IF2015 = 1.132)
  • Hofman, J., Kučera, R., Neumanova, Z., Klimeš, J., Ceckova, M., Staud, F. Placental passage of olomoucine II, but not purvalanol A, is affected by p-glycoprotein (ABCB1), breast cancer resistance protein (ABCG2) and multidrug resistance-associated proteins (ABCCs). Xenobiotica. 2016; 46(5): 416-423. (IF2015= 1.723)
  • Macháček, M., Kollár, J., Miletin M., Kučera R., Kubat, P., Šimůnek T., Nováková V., Zimčík P. Anionic hexadeca-carboxylate tetrapyrazinoporphyrazine: synthesis and in vitro photodynamic studies of a water-soluble, non-aggregating photosensitizer, RSC Advances. 2016; 6(12): 10064-10077. (IF2015= 3.289)


  • Cihalova, D., Ceckova, M., Kučera, R., Klimeš, J., Staud, F. Dinaciclib, a cyclin-dependent kinase inhibitor, is a substrate of human ABCB1 and ABCG2 and an inhibitor of human ABCC1 in vitro. Biochemical Pharmacology. 2015; 98(3): 465-472. (IF2015= 5.091)
  • Vachova, L., Machacek, M., Kučera, R., Demuth, J.; Cermak, P., Kopecky, K., Miletin, M., Jedlickova, A., Simunek, T., Novakova, V., Zimcik, P. Heteroatom-substituted tetra(3,4-pyrido)porphyrazines: a stride toward near- infrared-absorbing macrocycles. Organic & Biomolecular Chemistry. 2015; 13(20): 5608-5612.  (IF2015=3.559)
  • Macháček, M., Cidlina A., Nováková V., Švec J., Rudof E., Miletin M., Kučera R., Šimůnek T., Zimčík P. Far-Red-Absorbing Cationic Phthalocyanine Photosensitizers: Synthesis and Evaluation of the Photodynamic Anticancer Activity and the Mode of Cell Death Induction. Journal of Medicinal Chemistry. 2015; 58(4): 1736-1749. (IF2015= 5.589)
  • Bureš J., Jansová H., Stariat J., Filipský T., Mladěnka P., Šimůnek T., Kučera R., Klimeš J., Wang Q., Franz K.J., Kovaříková P. LC-UV/MS methods for the analysis of prochelator-boronyl salicylaldehyde isonicotinoyl hydrazone (BSIH) and its active chelator salicylaldehyde isonicotinoyl hydrazone (SIH). Journal of Pharmaceutical and Biomedical Analysis. 2015; 105(1): 55-63. (IF2015= 3.169)
  • Servusova-Vanaskova, B., Jandourek, O., Paterova, P., Kordulakova, J., Plevakova, M., Kubicek, V., Kučera, R., Garaj, V., Naesens, L., Kunes, J., Dolezal, M., Zitko, J. Alkylamino derivatives of N-benzylpyrazine-2-carboxamide: synthesis and antimycobacterial evaluation. Medchemcomm. 2015; 6(7): 1311-1317. (IF2015= 2.319)


  • Kučera R., Kovaříková P., Pasáková-Vrbatová .I, Slaninová J., Klimeš J. Zirconia-a stationary phase capable of the separation of polar markers of myocardial metabolism in hydrophilic interaction chromatography. Journal of Separation Science. 2014, 37(9-10):1089-93. (IF2014= 2.737)
  • Servusová-Vanasková, B., Paterová, P., Mandikova, J., Plevakova, M., Kubicek, V., Kučera, R., Kunes, J., Dolezal, M., Zitko, J. Alkylamino derivatives of pyrazinamide: Synthesis and antimycobacterial evaluation. Bioorganic & Medicinal Chemistry Letters. 2014; 24(2): 450-453. (IF2014= 2.420)


  • Zitko, J., Servusová, B., Paterová, P., Mandikova, J., Kubicek, V., Kučera, R., Hrabcova, V., Kunes, J., Soukup, O., Dolezal, M., Synthesis, Antimycobacterial Activity and In Vitro Cytotoxicity of 5-Chloro-N-phenylpyrazine-2-carboxamides, Molecules. 2013; 18(12): 14807-14825. (IF2013=2.095)
  • Hofman J., Kucera R., Cihalova D., Klimes J., Ceckova M., Staud F. Olomoucine II, but Not Purvalanol A, Is Transported by Breast Cancer Resistance Protein (ABCG2) and P-Glycoprotein (ABCB1), Plos one. 2013; 8(10). (IF2013=3.534)
  • Nobilis M., Mikušek J., Szotáková B., Jirásko R., Holčapek M., Chamseddin C., Jira Th., Kučera R., Kuneš J., Pour M. Analytical power of LLE-HPLC-PDA-MS/MS in drug metabolism studies: Identification of new nabumetobe metabolites, Journal of Pharmaceutical and Biomedical Analysis. 2013; 80:164-172. (IF2013=2.829)
  • Cahlikova L., Locarek M., Benesova N., Kucera R., Chlebek J., Novak Z., Opletal L. Isolation and Cholinesterase Inhibitory Activity of Narcissus Extracts and Amaryllidaceae Alkaloid, Natural Product Communications. 2013; 8(6):781-785. (IF2013= 0.924)
  • Stariat J., Kovaříková P., Kučera R., Klimeš J., Kalinowski D.S, Richardson D.R, Ketola R.A. Identification of in vitro metabolites of the novel anti-tumor thiosemicarbazone, DpC, using ultra-high performance liquid chromatography-quadrupole-time-of-flight mass spectrometry, Analytical and Bioanalytical Chemistry. 2013; 405(5):1651-61. (IF2013= 3.578)


  • Kalafut P., Kučera R., Klimeš J. The Retention Behavior of Acidic, Basic and Neutral Pharmaceuticals on the Deactivated Polybutadiene Zirconia Phase, Current Analytical Chemistry. 2012; 8(4): 574-582. (IF2012: 1.558)
  • Kalafut P., Kučera R., Klimeš J. The influence of a carbon layer deposited on a zirconia surface on the retention of polar analytes in an organic rich mobile phase , Journal of Chromatography A. 2012; 1232 : 242-247. (IF2012: 4.612)
  • Cahlíková L., Benešová N., Macáková K., Kučera R., Hrstka V., Klimeš J., Jahodář L., Opletal L. Alkaloids from some amaryllidaceae species and their cholinesterase activity, Natural Product Communications. 2012; 7(5): 571-574. (IF2012: 0.956)
  • Cahlíková L., Kučera R., Hošťálková, A.,Klimeš J., Opletal L. Identification of Pavinane Alkaloids in the Genera Argemone and Eschscholzia by GC-MS Natural Product Communications. 2012; 7(10): 1279-1281. (IF2012: 0.956)
  • Novakova V., Kobak R.Z.U., Kučera R., Kopecky K., Miletin M., Krepsová V., Ivincová J., Zimcik P. The effect of the number of carbohydrate moieties on the azaphthalocyanine properties, Dalton Transactions. 2012;  41: 10596-10604. (IF2012: 3.806)


  • Kučera R., Kovaříková P., Klivický M., Klimeš J. The retention behaviour of polar compounds on zirconia based stationary phases under hydrophilic interaction liquid chromatography conditions, Journal of Chromatography A. 2011; 1218(39): 6981-6986. (IF2011: 4.531)
  • Kopecky K., Novakova V., Miletin M., Kučera R., Zimcik P. Synthesis of new azaphthalocyanine dark quencher and evaluation of its quenching efficiency with different fluorophores, Tetrahedron. 2011; 67(33): 5956-5963. (IF2011: 3.025)
  • Zítko J., Doležal M., Svobodová M., Vejsová M., Kuneš J., Kučera R., Jílek P. Synthesis and antimycobacterial properties of N-substituted 6-amino-5-cyanopyrazine-2-carboxamides, Bioorganic & Medicinal Chemisty. 2011; 19(4): 1471-1476. (IF2011: 2.921)


  • Kopecky K., Novakova V., Miletin M., Kučera R., Zimcik P. Solid-Phase Synthesis of Azaphthalocyanine-Oligonucleotide Conjugates and Their Evaluation As New Dark Quenchers of Fluorescence, Bioconjugate Chemistry. 2010; 21(10): 1872-1879. (IF2010: 5.002)


  • Kalafut P., Kučera R., Klimeš J., Sochor J. An innovative approach to the analysis of 3-[4-(2-methylpropyl)phenyl]propanoic acid as an impurity of ibuprofen on a carbon-coated zirconia stationary phase, Journal of Pharmaceutical and Biomedical Analysis. 2009; 49(5): 1150- 1156. (IF2009: 2.453)
  • Žižkovský V., Kučera R., Klimeš J., Holásková P. Dohnal J. RP-ZrO2 stationary phase as an alternative to separate of doxazosin impurities, Chromatographia. 2009; 70(1-2): 185-189. (IF2009: 1.098)
  • Pasáková I., Kovaříková P., Kučera R., Klimeš J., Sochor J., Hrábalek A. Development and validation of an LC-ESI-MS ion-trap method for analysis of impurities in Transkarbam 12, a novel transdermal accelerant, Chromatographia. 2009; 96(9-10): 977 – 983. (IF2009: 1.098)


  • Žižkovský V., Kučera R., Klimeš J., Dohnal J. Titania-based stationary phase in separation of ondansetron and its related compounds, Journal of Chromatography A. 2008; 1189(1-2): 83-91. (IF2008: 3.756)


  • Zerzaňová A., Žižkovský V., Kučera, R., Klimeš J., Jesenský I., Dohnal J., Barrón D. Using of HPLC coupled with coulometric detector for the determination of biotin in pharmaceuticals,  Journal of Pharmaceutical and Biomedical Analysis. 2007; 45(5): 730-735. (IF2007: 2.761)
  • Žižkovský V., Kučera R., Klimeš J., Dohnal J. Potential employment of non-silica-based stationary phases in pharmaceutical analysis, Journal of Pharmaceutical and Biomedical Analysis. 2007; 44(5): 1048-1055. (IF2007: 2.761)


  • Garcés A., Zerzaňová A., Kučera, R., Barrón, D., Barbosa, J. Determination of a series of quinolones in pig plasma using solid-phase extraction and liquid chromatography coupled with mass spectrometric detection. Application to pharmacokinetic studies,  Journal of Chromatography A. 2006; 1137(1): 22-29. (IF2006: 3.554)


  • Kučera, R., Žižkovský, V.,  Sochor, J., Klimeš, J., Dohnal, J. Utilization of zirconia stationary phase as a tool in drug control, Journal of Separation Science. 2005; 28: 1307-1314. (IF2005: 1.829)
  • Kučera, R., Sochor, J., Klimeš, J., Dohnal, J. Use of the zirconia-based stationary phase for separation of ibuprofen and its impurities, Journal of Pharmaceutical and Biomedical Analysis. 2005; 38: 609-618. (IF2005: 1.889)
  • Kučera R., Nobilis M., Skálová L., Szotáková B., Císař P., Jira Th., Klimeš J., Wsól V. Use of chiral reversed-phase liquid chromatography for the evaluation of stereoselectivity in the karbonyl reduction of potential benzo(c)fluorene antineoplastics benfluron in various species, Journal of Pharmaceutical and Biomedical Analysis. 2005; 37: 1049-1057. (IF2005: 1.889)

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