Education
- Since 2022 – postgraduate study at Charles University, Faculty of Pharmacy, Department of Pharmacognosy and Pharmaceutical Botany in the study program: Pharmacognosy and Nutraceuticals. The theme of Doctoral Thesis: Isolation of selected indole alkaloids and their biological activity
- 2014–2022 – Masters’s degree in Pharmacy at Charles University, Faculty of Pharmacy. The theme of Diploma Thesis: Alkaloids of the family Amaryllidaceae and their biological activity II.
Research interests
- Secondary plant metabolites: alkaloids, monoterpenes, sesquiterpenes and diterpenes
- Optimization of sample preparation and separation methods, including gas chromatography (GC), ultra-high performance liquid chromatography (UHPLC) and ultra-high performance supercritical fluid chromatography (UHPSFC) with MS, MS/MS and HRMS for various phytochemical and plant-related applications
Current position
Previous position
Research fellowship
- 07/2023 – 09/2023 – Graduate institute of Natural Products, College of Pharmacy, Kaohsiung Medical University, Taiwan; Research internship focused on rapid separation and analysis of alkaloids from various Barberry species
Professional membership
- since 2024 – American Society of Pharmacognosy
- since 2022 – Czech Apothecary Association
Publications
Original papers
2026
TIMBILLA, A.A., PIDANY, F., KOHELOVA, E., KROUSTKOVA, J., KRALOVEC, K., RATAJ, J., CECKOVA, M., MAAFI, N., LOPEZ, V., LANGA, C.M., KOSTURKO, S., JENCO, J., MUTHNA, D., KOUTOVA, D., REZACOVA, M., CAHLIKOVA, L., CHLEBEK, J., HAVELEK, R.: Harmine derivatives as anticancer agents endowed with potent and selective antileukemia activity: synthesis, biological evaluation, proapoptopic and genotoxic activity. Arch. Pharm. 359, e70197 (2026). https://doi.org/10.1002/ardp.70197
2025
SAFRATOVA, M., CHEN, Y.L., HOSTALKOVA, A., CHLEBEK, J., HSIEH, C.F., CHEN, B.H., CAHLIKOVA, L., KOSTURKO, S., BACKLUND, A., HORNG, J.Z., HWANG, T.L., KORINEK, M.: Anticoronavirus isoquinoline alkaloids: Unraveling the secrets of their structure-activity relationship. Influenza Other Respir. Viruses 19, e70166 (2025). https://doi.org/10.1111/irv.70166
KIMIZIBEKMEZ, H., ARU, B., KŘOUSTKOVÁ, J., ERDOGAN, M., TORRENCE, I., ANDO, K., TANTILLO, D.J., MALANÍK, M., KOSTURKO, Š., KUNEŠ, J., CAHLÍKOVÁ, L.: Cytotoxic stilbenoids, hetero- and homodimers of homoisoflavonoids from Prospero autumnale. J. Nat. Prod. 88, 458-468 (2025). https://doi.org/10.1021/acs.jnatprod.4c01263
PILAŘOVÁ, V., BURIANOVÁ, K., KOSTURKO, Š., KULKARNI, M., VAN STADEN, J., NOVÁKOVÁ, L.: Supercritical fluids in extraction, purification, and separation of selected Amaryllidaceae alkaloids; Sep. Pur. Technol. 377(2), 133968 (2025); https://doi.org/10.1016/j.seppur.2025.133968
KIMIZIBEKMEZ, H., ARU, B., KŘOUSTKOVÁ, J., ERDOGAN, M., TORRENCE, I., ANDO, K., TANTILLO, D.J., MALANÍK, M., KOSTURKO, Š., KUNEŠ, J., CAHLÍKOVÁ, L.: Cytotoxic stilbenoids, hetero- and homodimers of homoisoflavonoids from Prospero autumnale. J. Nat. Prod. 88, 458-468 (2025). https://doi.org/10.1021/acs.jnatprod.4c01263
2024
KŘOUSTKOVÁ, J., KOHELOVÁ, E., MUTHNÁ, D., KUNEŠ, J., HAVELEK, R., VRABEC, R., MALANÍK, M., SUCHÁNKOVÁ, D., CHLEBEK, J., JENČO, J., KOSTURKO, Š., CAHLÍKOVÁ, L. (2024). Undescribed Amaryllidaceae alkaloids from Zephyranthes citrina and their cytotoxicity. J. Nat. Prod. 87, 2317-2326 (2024). https://doi.org/10.1021/acs.jnatprod.4c00825
PILAŘOVÁ, V., PLACHKÁ, K., HERBSOVÁ, D., KOSTURKO, Š., ŠVEC, F., NOVÁKOVÁ, L.: Comprehensive two-step supercritical fluid extraction for green isolation of volatiles and phenolic compounds from plant material; Green Chem. 26, 6480-6489 (2024). https://doi.org/10.1039/D4GC00371C
Reviews
2025
VRABEC, R., DRASAR, P., OPLETAL, L., KOSTURKO, S., BLUNDEN, G., CAHLÍKOVÁ, L.: Alkaloids from the genus Vinca L. (Apocynaceae): a comprehensive biological and structural review. Phytochem. Rev. 24, 3665-3735 (2025). https://doi.org/10.1007/s11101-025-10102-z
Conferences
2026
- 03.02.2026 – 04.02.2026 – 16th Postgraduate and Postdoc Conference, Faculty of Pharmacy in Hradec Králové. Short lecture: Comprehensive UHPLC-HRMS and UHPLC-MS/MS Profiling of Isoquinoline Alkaloids in Barberry Species
2025
- 07.09.2025 – 12.09.2025 – 26th School of Mass Spectrometry, Špindlerův Mlýn. Poster: UHPLC-MS Exploration of Isoquinoline Alkaloids: Can HRMS Using Q-TOF Even Come Close to Triple Quadrupole Sensitivity?
- 02.06.2025 – 06.06.2025 – Phytochemical Society of Europe’s Young Scientist Meeting – Trends in Natural Product Research, Wrocław. Poster: Isoquinoline Alkaloids: Unlocking the Potential of Ultra-High Performance Supercritical Fluid Chromatography in the Analysis of Quaternary Compounds (P36)
- 18.05.2025 – 21.05.2025 – Czech Chromatographic School – HPLC.cz 2025, Zaječí. Short lecture: The Importance of GC in the Control and Characterization of Essential Oils and Herbal Drugs
2024
- 13.07.2024 – 17.07.2024 – International Congress on Natural Products Research, Kraków. Poster: Revealing the veil of Barberry Alkaloids: High-Throughput non-targeted UHPLC-DIA-HRMS Profiling Enhanced by Solid Phase Extraction based on Mixed-Mode Sorbent (S2.P88)
- 30.01.2024 – 31.01.2024 – 14th Postgraduate and Postdoc Conference, Faculty of Pharmacy in Hradec Králové. Short lecture: Unraveling Alkaloid Profiles in Diverse Barberry Species‘: UHPLC-HRMS Amplified by Weak Cation Mixed-Mode Solid Phase Extraction
Courses and Training
2025
- 08.09.2025 – 26th School of Mass Spectrometry, Špindlerův Mlýn. Course: Mass spectrometric imaging
- 07.09.2025 – 26th School of Mass Spectrometry, Špindlerův Mlýn. Course: Modern chromatographic methods in combination with mass spectrometry
2024
- 03.09.2024 – 05.09.2024 – Pragolab s.r.o., Prague, Czech Republic. Practical course School SW Chromeleon 7 used in LC/IC/GC/MS
2023
- 26.04.2023 – 27.04.2023 – Pragolab s.r.o., Prague, Czech Republic. Practical course HPLC school