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A vacuum evaporator is used for preparation of summary ethanolic plant extracts in a preparative measurement.
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Further, flash chromatography (Sepacore X10 system; Buchi) with UV and ELS detectors is used for purification of summary alkaloidal plant extracts and obtained fractions.
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Furthermore, smaller fractions are fractionated by flash chromatography using puriFlash® 535-XL.
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Preparative HPLC-PDA-MS (Waters) for separation of compounds from fractions. HPLC is also possible used for identification and determination of purity of isolated compounds or screening of plant extracts.
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Semi-preparative HPLC for alkaloid mixture separations in several mg.
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Optical rotation of pure and structurally determined alkaloids with chiral centres is measured with an automatic polarimeter.
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HPTLC is used for screening of plant extracts/fractions and allows also semi-quantitative determination of compounds and screening of biological activities of metabolites in extracts.
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GC-MS for the determination of a profile of some plant extract alkaloids.
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Reader Synergy serves for in vitro biological assays (enzymes).
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Synergy Neo2 Multi-Mode Reader in vitro biological assays (enzymes, cell cultures).
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| A laminar box for evaluation of cytotoxic activity of isolated alkaloids. |
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