Microscopy - confocal laser scanning microscope - Nikon Eclipse Ti, fluorescence microscope Olym-pus BX40.
Cultivation of microbes - CO2 incubator, microbiological incubators, autoclaves, biohazard safety boxes, anaerostat.
Mapping of the growth of microbes - culture growth monitoring instrument - Bioscreen C, spectro-photometer.
And other: vortexes, OD meters, microcentrifuge, cooling centrifuge, Stirred cell for sam-ple/protein concentration, microbiology shakers, sonification bath, …
Methods:
Microdilution broth method, disk diffusion test, E-test, aerobic/microaerobic/anaerobic microbes cultivation, microbial population counting, mapping of growth curves
In vitro biofilm formation, biofilm quantification – spectrophotometrically; detection, visualization – fluorescent, confocal microscopy.
Evaluation of metabolic activity of microbes – spectrophotometrically, microbial viability evaluation – plate count method, bright field/fluorescent microscopy.
Microbial protein isolation, concentration and purification: Stirred cell, Amicon, Centricon (Millipore) tubes, sucrose gradient; preparation of protein samples for MS analysis – sample desalting, protein separation and visualization: 1 D SDS-PAGE, Coomassie blue staining, sweet silver staining – 1 D gel analysis; in-gel trypsin digest, extraction/concentration of peptides.