Our research project is based on the synthesis of novel potentially active antituberculous drugs to obtain the structures with the higher biological activity against M. tuberculosis and/or M. avium, M. kansasii, on the searching of optimal structural modifications of pyrazinamide, the model compound. In vitro evaluation of the antituberculous activity is carried out within the Institute of Clinical Microbiology University Hospital, Hradec Kralove. Project component is molecular modelling of active compounds for better understanding of their mechanism of action on molecular level and calculations and/or experimental determination of their lipophilicity data. All prepared compounds are tested on the antifungal/antibacterial susceptibility against selected strains, too. A part of the project is the cytotoxicity evaluation of the most active compounds.
Other research activities are focused on the study of inhibition of photosynthetic electron transport in spinach chloroplasts in substituted amides of pyrazinecarboxylic acid, searching of new elecitors derived from pyrazine, and design and synthesis of reactivators and inhibitors of cholinesterases.
The research topics of the group of microbiology and immunology are focused on testing of antimicrobial activity of newly synthetized substances at the faculty of Pharmacy in Hradec Králové. Another science topic includes the study of the pathogenesis of Candida albicans infections and the role of extracellular vesicles of C. albicans in pathogenesis of vulvovaginal candidiasis.