PharmDr. Lukáš Lochman, Ph.D.

Research focus

• Separation techniques - LC-UV/DAD, LC-FD, LC-MS, CE-DAD
• Analytical and bioanalytical method development and validation 
• Development of new stationary phases based on graphene

• HPLC purification of the oligonucleotide probes based on azaphthalocyanines (AzaPcs)

Education and professional carrier

• Since 2017  Assistant profesor at Department of Pharmaceutical Chemistry and Pharmaceutical Analysis
• Since 2017  member of Pharmaceutical Analysis Group
• 2016 – 2017 Assistant at Department of Biophysics and Physical Chemistry

• 2013 – 2018 member of Azaphthalocyanine group

• 2013 – 2018 Ph.D. in Pharmaceutical Chemistry - Faculty of Pharmacy in Hradec Kralove, Charles University                                             (Thesis: Study of recognition moieties of sensoric azaphthalocyanines; supervisor: Assoc. Prof. PharmDr. Veronika Novakova, Ph.D.)
• 2008 – 2013 Mgr. (equiv. MS.c.) in Pharmacy - Faculty of Pharmacy in Hradec Kralove, Charles University (Diploma Thesis: Synthesis of azaphthalocyanines with photophysical properties sensitive to metal cations; supervisor: Assoc. Prof. PharmDr. Petr Zimcik, Ph.D.)

Grant projects

• 2024 – 2029 Charles University: UNCE project "ADVanced models, Experimental apporaches and bioinformatics in pharmacological ReSEsearch (ADVERSE).‘‘ UNCE24/MED/008 (co-worker)

• 2014 – 2016 Grant Agency of Charles University: Study of recognition moieties of sensoric azaphthalocyanines. GAUK: 494214 (principal investigator)

• 2014 – 2016 Czech Science Foundation: Development of new photosensitizers for photodynamic therapy and investigation of their mechanism of action on cellular level. GAČR: 13-27761S (co-worker)

• Since 2014:  Co-worker of several other grants: Grant Agency of Charles University (GAUK: 1182313; GAUK: 1916214).

• 2013 – 2017 Charles University: UNCE project "Centre for investigation of toxic and protective effects of drugs on cardiovascular system.‘‘ UNCE 33/2012 (co-worker)

Educational activities

• 2023 - 2024        Preparation of new study materials - "Laboratory protocols" (Project: NPO_UK_MSMT-16602/2022)
• Since 2023          Subject guarantor: Analysis of exogenous substances in biol. material (GB165)
• Since 2017          Tutor of practical training from Pharmaceutical Analysis

• Since 2016          Consultant of Diploma Theses at Faculty of Pharmacy in Hradec Kralove

• 2016 – 2017       Tutor of practical training from Biophysics and Physical Chemistry
• 2013 – 2015       Tutor of practical training from Pharmaceutical Chemistry

• 2024                    Certificate (University of Hradec Kralove): AI friendly and without secrets: How can it change our world?
• 2021                    Centre for Lifelong Learning (Charles University): Management skills development program.
• 2020                    Centre for Lifelong Learning (Charles University): Educational program of pedagogical skills.

Awards/Prizes

• 2019 - Faculty medal - The medal of the Faculty of Pharmacy for excellent scientific results and faculty development

• 2019 - Bolzano Award (University Award) in biomedical disciplines category

• 2018 - Josef Hlavka Award for the Best Students and Graduates  

• 2017 - 2^nd place in the prestigious competition Sanofi Prize for Pharmacy 2017 arranged by French Embassy in the Czech Republic  and Jean-Marie Lehn (laureate of Nobel Prize) 

• 2017 - Medal of the City of Hradec Kralove for scientific work

Research fellowship

• 2019 - Institut Charles Sadron CNRS, University of Strasbourg (Strasbourg, France) – Layer-by-layer assembly technique for capillary wall modification usable in separation aplications. Prof. Gero Decher (3 months)
• 2016 - Graz University of Technology (Graz, Austria) – The study of possibility to use azaphthalocyanine indicators in CO₂ sensors.           Ass. Prof. Sergey Borisov (1 month)
• 2015 - Graz University of Technology (Graz, Austria) – The application of azaphthalocyanine indicators in fluorescent sensors for metal cations. Ass. Prof. Sergey Borisov (3 months)

Publications

Journals with impact factor (∑IF = 49.945; ØIF = 5.549):

• two other manuscripts in finalization

1. Smutny T, Smutna L, Lochman L, Kamaraj R, Kucera R, Pavek P. Rifampicin and its derivatives: stability, disposition, and affinity towards pregnane X receptor employing 2D and 3D primary human hepatocytes. Biochemical Pharmacology. 2024; 229 (116500): 1-14. (IF₂₀₂₃ = 5.300; AIS: 1.109)
2. Váňová N, Múčková L, Kalíšková T, Lochman L, Bzonek P, Švec F. In Vitro Evaluation of Oxidative Stress Induced by Oxime Reactivators of Acetylcholinesterase in HepG2 Cells. Chemical Research in Toxicology. 2023; 36 (12): 1912-1920. (IF₂₀₂₂ = 4.100; AIS: 0.771)
3. Pavek P, Dusek J, Smutny T, Lochman L, Kucera R, Skoda J, Smutna L, Kamaraj R, Soucek P, Vrzal R, Dvorak Z. Gene Expression Profiling of 1α,25(OH)₂D₃ Treatment in 2D/3D Human Hepatocyte Models Reveals CYP3A4 Induction but Minor Changes in Other Xenobiotic-Metabolizing Genes. Molecular Nutrition and Food Research. 2022; 2200070: 1-17. (IF₂₀₂₁ = 6.575; AIS: 1,009)
4. Horáček O, Papajová-Janetková M, Grüner B, Lochman L, Štěrbová-Kovaříková P, Vespalec R, Kučera R. The first chiral HPLC separation of dicarba-nido-undecarborate anions and their chromatographic behavior. Talanta. 2021; 222: 1-9. (IF₂₀₂₀ = 6.057; AIS: 0.828)
5. Lochman L, Machacek M, Miletin M, Uhlířová Š, Lang K, Kirakci K, Zimcik P, Novakova V. Red-Emitting Fluorescence Sensors for Metal Cations: The Role of Counteranions and Sensing of SCN⁻ in Biological Materials. ACS Sensors. 2019; 4 (6): 1552-1559.                        (IF₂₀₁₈ = 6.944; AIS: 1.462)
6. Lochman L, Zimcik P, Klimant I, Novakova V, Borisov SM. Red-emitting CO₂ sensors with tunable dynamic range based on pH-sensitive azaphthalocyanine indicators. Sensors and Actuators B: Chemical. 2017; 246: 1100-1107. (IF₂₀₁₆ = 5.401; AIS: 0.786)
7. Lochman L, Svec J, Roh J, Kirakci K, Lang K., Zimcik P, Novakova V. Metal cation recognition in water by a tetrapyrazinoporphyrazine-based tweezer receptor. Chemistry - A European Journal. 2016; 22 (7): 2417-2426. (IF₂₀₁₅ = 5.771; AIS: 1.407)
8. Lochman L, Svec J, Roh J, Novakova V. The role of the size of aza-crown recognition moiety in azaphthalocyanine fluorescence sensors for alkali and alkaline earth metal cations. Dyes and Pigments. 2015; 121: 178-187. (IF₂₀₁₄ = 3.966; AIS: 0.629)
9. Novakova V, Lochman L, Zajícová I, Kopecky K, Miletin M, Lang K, Kirakci K, Zimcik P. Azaphthalocyanines: Red Fluorescent probes for Cations. Chemistry - A European Journal. 2013; 19 (16): 5025-5028. (IF₂₀₁₂ = 5.831; AIS: 1.478)