PharmDr. Lukáš Lochman, Ph.D.

Research focus

• Separation techniques - HPLC, CE
• Development of new stationary phases based on graphene

• HPLC purification of the oligonucleotide probes based on azaphthalocyanines (AzaPcs)

• Development of new sensoric devices based on AzaPc indicators
• Synthesis of asymmetrical AzaPcs usable as fluorescence sensors
• Study of photophysical and photochemical properties of AzaPcs

Education and professional carrier

• Since 2017  Assistant profesor at Department of Pharmaceutical Chemistry and Pharmaceutical Analysis
• Since 2017  member of Pharmaceutical Analysis Group
• 2016 – 2017 Assistant at Department of Biophysics and Physical Chemistry

• 2013 – 2018 member of Azaphthalocyanine group

• 2013 – 2018 Ph.D. in Pharmaceutical Chemistry - Faculty of Pharmacy in Hradec Kralove, Charles University                                             (Thesis: Study of recognition moieties of sensoric azaphthalocyanines; supervisor: Assoc. Prof. PharmDr. Veronika Novakova, Ph.D.)
• 2008 – 2013 Mgr. (equiv. MS.c.) in Pharmacy - Faculty of Pharmacy in Hradec Kralove, Charles University (Diploma Thesis: Synthesis of azaphthalocyanines with photophysical properties sensitive to metal cations; supervisor: Assoc. Prof. PharmDr. Petr Zimcik, Ph.D.)

Grant projects

• 2014 – 2016 Grant Agency of Charles University: Study of recognition moieties of sensoric azaphthalocyanines. GAUK: 494214 (principal investigator)

• 2014 – 2016 Czech Science Foundation: Development of new photosensitizers for photodynamic therapy and investigation of their mechanism of action on cellular level. GAČR: 13-27761S (co-worker)

• Since 2014:  Co-worker of several other grants: Grant Agency of Charles University (GAUK: 1182313; GAUK: 1916214).

• 2013 – 2017 Charles University: UNCE project Centre for investigation of toxic and protective effects of drugs on cardiovascular system.‘‘ UNCE 33/2012 (co-worker)

Professional membership

• since 2013 - Society of Porphyrins and Phthalocyanines

Educational activities

• Since 2017          Tutor of practical training from Pharmaceutical Analysis

• Since 2016          Consultant of Diploma Theses at Faculty of Pharmacy in Hradec Kralove (2×)

• 2016 – 2017       Tutor of practical training from Biophysics and Physical Chemistry
• 2013 – 2015       Tutor of practical training from Pharmaceutical Chemistry

• 2020                    Centre for Lifelong Learning (Charles University): Educational program of pedagogical skills.
• 2021                    Centre for Lifelong Learning (Charles University): Management skills development program.

Awards/Prizes

• 2019 - Faculty medal - The medal of the Faculty of Pharmacy for excellent scientific results and faculty development

• 2019 - Bolzano Award (University Award) in biomedical disciplines category

• 2018 - Josef Hlavka Award for the Best Students and Graduates  

• 2017 - 2^nd place in the prestigious competition Sanofi Prize for Pharmacy 2017 arranged by French Embassy in the Czech Republic  and Jean-Marie Lehn (laureate of Nobel Prize) 

• 2017 - Medal of the City of Hradec Kralove for scientific work

Research fellowship

• 2019 - Institut Charles Sadron CNRS, University of Strasbourg (Strasbourg, France) – Layer-by-layer assembly technique for capillary wall modification usable in separation aplications. Prof. Gero Decher (3 months)
• 2016 - Graz University of Technology (Graz, Austria) – The study of possibility to use azaphthalocyanine indicators in CO₂ sensors.           Ass. Prof. Sergey Borisov (1 month)
• 2015 - Graz University of Technology (Graz, Austria) – The application of azaphthalocyanine indicators in fluorescent sensors for metal cations. Ass. Prof. Sergey Borisov (3 months)

Publications

Journals with impact factor (∑IF = 40.545; ØIF = 5.792):

1. Pavek P, Dusek J, Smutny T, Lochman L, Kucera R, Skoda J, Smutna L, Kamaraj R, Soucek P, Vrzal R, Dvorak Z. Gene Expression Profiling of 1α,25(OH)₂D₃ Treatment in 2D/3D Human Hepatocyte Models Reveals CYP3A4 Induction but Minor Changes in Other Xenobiotic-Metabolizing Genes. Molecular Nutrition and Food Research. 2022; 2200070: 1-17. (IF₂₀₂₁ = 6.575; AIS: 1,009)
2. Horáček O, Papajová-Janetková M, Grüner B, Lochman L, Štěrbová-Kovaříková P, Vespalec R, Kučera R. The first chiral HPLC separation of dicarba-nido-undecarborate anions and their chromatographic behavior. Talanta. 2021; 222: 1-9. (IF₂₀₂₀ = 6.057; AIS: 0.828)
3. Lochman L, Machacek M, Miletin M, Uhlířová Š, Lang K, Kirakci K, Zimcik P, Novakova V. Red-Emitting Fluorescence Sensors for Metal Cations: The Role of Counteranions and Sensing of SCN⁻ in Biological Materials. ACS Sensors. 2019; 4 (6): 1552-1559.                        (IF₂₀₁₈ = 6.944; AIS: 1.462)
4. Lochman L, Zimcik P, Klimant I, Novakova V, Borisov SM. Red-emitting CO₂ sensors with tunable dynamic range based on pH-sensitive azaphthalocyanine indicators. Sensors and Actuators B: Chemical. 2017; 246: 1100-1107. (IF₂₀₁₆ = 5.401; AIS: 0.786)
5. Lochman L, Svec J, Roh J, Kirakci K, Lang K., Zimcik P, Novakova V. Metal cation recognition in water by a tetrapyrazinoporphyrazine-based tweezer receptor. Chemistry - A European Journal. 2016; 22 (7): 2417-2426. (IF₂₀₁₅ = 5.771; AIS: 1.407)
6. Lochman L, Svec J, Roh J, Novakova V. The role of the size of aza-crown recognition moiety in azaphthalocyanine fluorescence sensors for alkali and alkaline earth metal cations. Dyes and Pigments. 2015; 121: 178-187. (IF₂₀₁₄ = 3.966; AIS: 0.629)
7. Novakova V, Lochman L, Zajícová I, Kopecky K, Miletin M, Lang K, Kirakci K, Zimcik P. Azaphthalocyanines: Red Fluorescent probes for Cations. Chemistry - A European Journal. 2013; 19 (16): 5025-5028. (IF₂₀₁₂ = 5.831; AIS: 1.478)