Group of Experimental Pharmacology and Drug Interactions

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Karbanova S et al: Transport of ribavirin across the rat and human placental barrier: roles of nucleoside and ATP-binding cassette drug efflux transporters. 

Biochem Pharmacol. 2019 Feb 1. pii: S0006-2952(19)30030-9. doi: 10.1016/j.bcp.2019.01.024 

Group of experimental pharmacology and drug interactions prof PharmDr Frantisek Staud Ph.D., dr. Martina Ceckova, dr. Lukas Cerveny, dr. Jakub HofmanThe research of the Group of Experimental Pharmacology and Drug Interactions is mainly focused on pharmacotherapy during pregnancy, especially on drug transport across the placenta
 
Recently, we have started a new project, focusing on tryptophan and serotonin metabolism in the placenta and its importance for fetal programming. In this line of our research we investigate the effect of maternal pathologies (such as inflammation) or maternal medication (such as SSRIs/SNRIs antidepressants) on placental handling of serotonin and kynurenine.
 
Using the method of dually perfused rat term placenta we have constructed a pharmacokinetic model that allows for quantification of the role of placental transporters in transplacental pharmacokinetics. We tend to support our in situ and in vivo data by a battery of in vitro models using placenta-derived cell lines (BeWo, Jeg, Jar) or genetically manipulated MDCK cells. Using these approaches, we have described the functional expression of several ABC drug efflux transporters and SLC solute carriers in the trophoblast such as P-glycoprotein (MDR1/ABCB1), breast cancer resistance protein (BCRP/ABCG2), organic cation transporter 3 (OCT3/SLC22A3) and multidrug and toxin extrusion protein 1 (MATE1/SLC47A1).
 

Third line of our research follows the path of drug interactions on drug efflux transporters. Since several drug efflux transporters are involved in the multidrug resistance in cancer, we investigate interactions of cytotoxic drugs and their combinations on these transporters from the point of view of anticancer pharmacotherapy.

In most of our projects, in vivo as well as in vivo approaches and the common methods of molecular biology are employed - for detailed information see Equipment end methods.

We are open to collaborations in the area of transplacental pharmacokinetics and drug interactions; feel free to contact us.

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