Placenta in Health and Disease

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Our latest paper on "Serotonin homeostasis in the materno‐fetal interface at term: role of transporters (SERT/SLC6A4 and OCT3/SLC22A3) and monoamine oxidase A (MAO‐A) in uptake and degradation of serotonin by human and rat term placenta" is accepted for publication at Acta Physiologica, a highly prestigious journal in the field of Physiology!!

The Placenta in Health and Disease is mainly focused on pharmacotherapy during pregnancy, especially on drug transport across the placenta
Recently, we have started a new project, focusing on tryptophan and serotonin metabolism in the placenta and its importance for fetal programming. In this line of our research we investigate the effect of maternal pathologies (such as inflammation) or maternal medication (such as SSRIs/SNRIs antidepressants) on placental handling of serotonin and kynurenine.
Using the method of dually perfused rat term placenta we have constructed a pharmacokinetic model that allows for quantification of the role of placental transporters in transplacental pharmacokinetics. We tend to support our in situ and in vivo data by a battery of in vitro models using placenta-derived cell lines (BeWo, Jeg, Jar) or genetically manipulated MDCK cells. Using these approaches, we have described the functional expression of several ABC drug efflux transporters and SLC solute carriers in the trophoblast such as P-glycoprotein (MDR1/ABCB1), breast cancer resistance protein (BCRP/ABCG2), organic cation transporter 3 (OCT3/SLC22A3) and multidrug and toxin extrusion protein 1 (MATE1/SLC47A1).
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In most of our projects, in vivo as well as in vivo approaches and the common methods of molecular biology are employed - for detailed information see Equipment end methods.

We are open to collaborations in the area of transplacental pharmacokinetics and drug interactions; feel free to contact us.

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© Charles University, Faculty of Pharmacy in Hradec Králové, Akademika Heyrovského 1203, 500 05 Hradec Králové, Czech Republic
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