Metabolism of drugs, activity and regulation of drug-metabolizing enzymes (DME) and their role in drug interactions and resistance
- Identification of metabolites of drugs and other xenobiotics in animals, parasitic helminths and plants
- Drug biotransformation and transport studies
- Interactions of natural substances/ food supplements with drugs – effect of drugs, dietary supplements, natural compounds and other xenobiotics on expression and activity of DME or antioxidant enzymes
- Mechanisms of drug resistance in parasitic helminths
- Interactions of anthelmintics in plants with polyphenols synthesis and with antioxidant defence system
Drug metabolism studies in vitro and/or in vivo
- Antiproliferative and toxicity tests using various cultures of immortalized cells
- Tests of hepatotoxicity of drugs and other xenobiotics
- Preparation and incubation of precision-cut liver and intestinal slices
- Isolation and cultivation of primary cultures of hepatocytes
- Preparation of subcellular fractions (animal and human organs, parasites, plants, cell cultures)
- Assays of enzyme expression
- Assays of enzyme activities
- Identification of metabolites of drugs and other xenobiotics
Drug resistance studies in helminths
- In vivo animal studies
- Isolation of helminths from infected hosts
- Ex vivo cultivations of helminths
- Molecular parasitology
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