Faculty staff

Doc. PharmDr. Petr Zimčík, Ph.D. (group leader)
Petr coordinates all the work of the team. He is also trying to do at least something in the lab – synthesis, photophysical and photochemical measurements, liposomes preparation. The attempts are not always successful, in particular the synthetic ones (Am I old to work in the lab?)


Doc. PharmDr. Veronika Nováková, Ph.D.
Veronika is actually doing everything – starting from synthesis ending with liposomes preparation. She is our expert on photochemical and photophysical measurements. She explained phenomenon of ICT, developed the pH sensitive AzaPcs, sensors for metal cations and has found several synthetic approaches to AzaPcs with complicated synthesis.


Doc. PharmDr. Miroslav Miletín, Ph.D.
Mirek is our oligonucleotide expert. He works on the development of new quenchers of fluoerscence. He prepared also some new delivery systems for photosensitizers (micelles, nanoemulsions, ...). He is also interested in binding of fluorescent AzaPcs to oligonucleotides.


RNDr. Miloslav Macháček, Ph.D. - member of Molecular and Cellular Toxicity Group

Slávek focused mainly on in vitro testing on photodynamic activity of phthalocyanines and their aza-analogues. Beside essential characteristics (such as phototoxicity, dark toxicities, subcellular localization), he performs advanced in vitro studies describing the mode of cell death, oxidative stress and effect of photosenzitizers on particular cell organelles.


PhD students

Mgr. Jan Kollár

Honza focuses his synthetic work on the (aza)phthalocyanine derivatives substituted by anionic substituents as potential photosensitizers for photodynamic therapy.


Mgr. Jiří Demuth

Jura works on the synthesis of aza-analogues of phthalocyanines usable as dark quenchers in oligonucleotide probes. He participates also in preparation of unsymmetrical phthalocyanines for photodynamic therapy.


Mgr. Filip Kostelanský

Filip opened a new topic in our research group as he focused on the synthesis of new minor groow binders (MGB) and the study of their binding to oligonucleotide probes. His results may be useful in future e.g. for a quantitative PCR based assays. He closely cooperates on this topic with Miroslav Miletin.





Undergraduate students

Veronika Čermáková – synthesis of pH sensitive azaphthalocyanines
Alena Jančárová – synthesis and evaluation of photodynamic activity of sulfonated azaphthalocyanines
Martina Karlíková – synthesis of pH sensitive azaphthalocyanines
Lucia Kočiščáková – synthesis of non-peripherally substituted phthalocyanines
Filip Kostelanský - synthesis of amphiphilic low-symmetry phthalocyanines suitable for photodynamic therapy
Jana Kubačková - synthesis of azaphthalocyanines suitable for binding to oligonucleotides
Matěj Machan – synthesis of azaphthalocyanines as dark quenchers in oligonucleotide probes
Aneta Schimmerová - synthesis of low-symmetrical azaphthalocyanine for DNA probes labeling
Pavol Sirka - synthesis of azaphthalocyanines suitable for binding to oligonucleotides
Petra Šišmová - synthesis of hydrophilic azaphthalocyanine fluorescence sensors
Štěpánka Uhlířová - synthesis of azaphthalocyanine fluorescence sensors with selectivity increased towards different metal cations
Die Vu Hong (Mary) – synthesis of amphiphilic phthalocyanines
Vojtěch Valer - synthesis of pH sensitive azaphthalocyanines with phenol groups


Past members

Mgr. Lukáš Lochman, Ph.D.  (
Lukáš was working on azaphthalocyanine fluorescence sensors for metal cations during his Diploma thesis  and he chose the similar topic for his disertation. During PhD, he focused on sensor´s recognition moiety and developed fluorescence sensors highly specific to desired analytes. Now he accepted postion at the Farmaceutical analysis and works in analytical area.

Mgr. Antonín Cidlina, Ph.D.
Tonda’s Diploma thesis led to the synthesis of water soluble, non-aggregating cationic Pcs with photodynamic properties. During PhD, he prepared series of AzaPc suitable for studying the structure-activity relationships of ICT at AzaPcs.

Mgr. Lenka Váchová, Ph.D.
Lenka’s PhD was  focused on synthesis and properties of Pcs and AzaPcs with donor center for ICT and influence of the ICT by different macrocycle structure. She  also cooperated on the synthesis of new tetrapyridoporphyrazines.

Mgr. Jan Švec, Ph.D.
Honza defended his Ph.D. at Masaryk University in Brno under the supervision of assoc. prof. Ing. Vladimír Šindelář, Ph.D. (research topics: organic synthesis of cyclic compounds, their supramolecular arrangement and host-guest interactions). In our group, he focused on the photophysical study of sensoric AzaPcs sensitive to metal cations.

PharmDr. Kamil Kopecký, Ph.D.
„Camel" focused his research on development of new quenchers of fluorescence with AzaPc structure. He managed the conjugation of AzaPcs with oligonucleotides and the quenching experiments based on hybridization of complementary oligonucleotide strands labeled with AzaPc and fluorophores.

PharmDr. Miroslav Kostka, Ph.D.
„Bohuš“ worked in our group almost since beginning. He finished his PhD in 2007. Main focus of his work was in synthesis of phthalocyanines.

PharmDr. Zbyněk Musil, Ph.D.
Zbyněk helped us a lot with the syntheses of AzaPcs during his PhD studies (defended in 2008). For example, he found a synthetic way for substituted tetraquinoxalinoporphyrazines.

Rabia Zeynep Uslu Kobak, PhD
Rabia spent a short (7 months) postdoc in our lab together with her husband Nedat in 2010. She helped us with carbohydrates substituted AzaPcs and new strong red-emitting fluorophores.

© Charles University, Faculty of Pharmacy in Hradec Králové, Akademika Heyrovského 1203, 500 05 Hradec Králové, Czech Republic
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